What are the main uses of Medical Guanidine Hydrochloride?
Medical Guanidine Hydrochloride, or medical guanidine hydrochloride, has the following main uses:
Medical guanidine hydrochloride is widely used in the field of biochemical research. In the study of protein denaturation, it can damage the spatial structure of protein molecules and help researchers clarify the connection between protein structure and function. Because it can interact with the hydrophobic groups of protein molecules, it weakens the force that maintains the natural structure of proteins, so that proteins can unfold. This property is crucial in protein structure analysis.
In the field of nucleic acid research, medical guanidine hydrochloride also has important uses. It can be used for the extraction and purification of nucleic acids, and can effectively lyse cells and release nucleic acids from cells. At the same time, it can remove impurities such as proteins and polysaccharides, ensure the purity and quality of the extracted nucleic acid, and lay the foundation for subsequent nucleic acid analysis and detection.
Furthermore, in some virus research experiments, medical guanidine hydrochloride can be used to inactivate viruses. It can destroy the protein shell or envelope structure of the virus, causing the virus to lose its infection activity, which helps to safely carry out virus-related research and avoid biosafety risks caused by virus leakage.
In addition, in some drug development processes, medical guanidine hydrochloride can be used as a reaction reagent or additive. It can participate in specific chemical reactions, assist in the synthesis of drug molecules with specific structures, or assist in the regulation of drug stability and solubility during the pharmaceutical preparation process, providing assistance for the development of new drugs.
Medical Guanidine Hydrochloride
Medical Guanidine Hydrochloride, that is, medical guanidine hydrochloride, is used as follows:
Medical guanidine hydrochloride is used in the medical tract, and its use is quite critical. First, it is often used in experiments related to protein denaturation. In biochemical research, in order to change the spatial structure of proteins to explore the relationship between their structure and function, protein samples are often treated with an appropriate amount of medical guanidine hydrochloride solution. Usually formulated into a solution of a specific concentration, the concentration depends on the characteristics of the protein being studied, usually 2-8mol/L. Add the protein sample to this solution slowly, stir gently to ensure full contact, and after a certain period of reaction, the protein denaturation can be observed.
Second, it also plays an important role in the extraction of viral nucleic acid. When extracting viral nucleic acid, medical guanidine hydrochloride can destroy the protein shell of the virus and release the nucleic acid. Generally, the virus-containing sample is mixed with an appropriate amount of guanidine hydrochloride solution first. This process needs to be rapid and uniform to make the virus particles quickly lyse. Then, with other reagents, such as chloroform, the separation of nucleic acid and protein and other impurities is achieved by centrifugation, so as to obtain pure viral nucleic acid for subsequent detection and analysis, such as nucleic acid amplification detection.
Furthermore, in some cell experiments, medical guanidine hydrochloride can be used to treat cells and change the permeability of cell membranes. According to the experimental requirements, prepare a suitable concentration of guanidine hydrochloride solution and incubate with cells. It is very important to grasp the concentration and time. If the concentration is too high or the time is too long, it is easy to cause cell death; if the concentration is too low or the time is too short, the ideal permeability effect cannot be achieved. It is necessary to explore the best conditions through pre-experiments to make the cells achieve the expected permeability state, which is conducive to the entry and exit of related substances into and out of the cells, so as to carry out subsequent research on intracellular material transport and metabolism.
Medical Guanidine Hydrochloride Safety
The safety of guanidine hydrochloride for medical use is related to human life and health, and it is a matter of utmost importance. Guanidine hydrochloride is an organic compound, which has a wide range of uses in the medical field. It is often used as a protein denaturant to assist in the structure and function of protein.
However, its safety cannot be ignored. First, it is corrosive. If it is accidentally touched, it will damage the skin and mucous membranes. If it touches the skin directly, it can cause skin redness, swelling, pain, and even ulceration; if it enters the eyes, it will hurt the eyes and damage the vision, which is very harmful.
Second, if it inhales dust or smoke, the respiratory tract will bear the brunt. It can cause coughing, asthma, breathing difficulties, long inhalation or lung diseases, affecting respiratory function.
Furthermore, oral administration is also harmful. It can disturb the digestive system, cause nausea, vomiting, abdominal pain, diarrhea and other diseases, and even damage the gastrointestinal mucosa, endangering life.
However, in medical experiments and treatment, if used properly and strictly abide by the procedures, the risk can also be controlled. Those who use this product must wear protective equipment, such as gloves, masks, goggles, and do it in a well-ventilated place. Dispose of it properly to prevent pollution. In this way, it can be safe, so that guanidine hydrochloride can be used in medicine and avoid its harm.
What are the storage conditions of Medical Guanidine Hydrochloride?
For guanidine hydrochloride used in medicine, its storage conditions are of paramount importance. Guanidine hydrochloride has hygroscopicity and is easy to absorb water from the air, so it should be placed in a dry place. It is advisable to choose a place with dry air and constant humidity to avoid moisture and agglomeration, which will affect its quality and utility.
Furthermore, guanidine hydrochloride is under high temperature, or it will decompose. When storing, avoid hot topics. It is advisable to store in a cool place, and the temperature should not exceed 30 degrees Celsius. In this way, its chemical properties can be kept stable and it will not deteriorate due to heat.
And guanidine hydrochloride is also sensitive to light. Exposure to light, or cause its chemical reaction, damage its quality. Therefore, it must be stored in an opaque container and placed in a dark place to prevent it from coming into contact with light.
And because of its certain chemical activity, it should not be mixed with other chemicals. It should be stored separately to prevent interaction and accidental changes. Only by observing the storage regulations in this way can the quality and purity of guanidine hydrochloride be maintained, and it can be used for medical purposes to do its best.
Medical Guanidine Hydrochloride Reacts with Other Substances
Yizhi guanidine hydrochloride is active in nature and often responds to other substances.
Fuguanidine hydrochloride has strong chemical properties and is widely used in the field of biochemistry. It can meet proteins, break their structures, and mutate. This is because it can break the hydrogen bond in the protein, destroy the stability of its maintenance, and make it inactive.
It also reacts with nucleic acids. The power of guanidine hydrochloride can dissolve the double chain of nucleic acid, exposing the base and facilitating analysis.
In common reagents, when bases meet guanidine hydrochloride, they will react. The acid-base phase is combined, and the raw salt and water. If it is a strong base, the reaction will be strong.
And some metal ions also attract guanidine hydrochloride. The properties of metal ions, or complexing with groups in guanidine hydrochloride, form new structures and change properties.
In organic reagents, those with active hydrogen are also easy to react with guanidine hydrochloride. Active hydrogen partially interacts with guanidine hydrochloride and forms a different product.
In short, guanidine hydrochloride is often sympathetic to other substances in the chemical environment. Because of its structure and properties, it is necessary to be careful in experiments and production to prevent accidents.