1,4-Piperazinebis(Ethanesulfonic Acid),Disodium Salt

1% 2C4 - Piperazinebis%28Ethanesulfonic + Acid% 29% 2CDisodium + Salt is the disodium salt of 1,4-piperazine diethylsulfonate, which is a commonly used reagent in biochemical research. Its chemical properties are unique, let me tell you in detail.
This substance is white crystalline and is quite stable at room temperature. However, it may react when exposed to high temperature or strong oxidants. It has good solubility and is easily soluble in water to form a clear solution. This property makes it widely used in the preparation of solutions for biochemical experiments.
Its aqueous solution has buffering properties and can maintain a specific pH range. Generally, it can be effectively buffered between pH 6.1 and 7.5. This buffering ability is derived from the interaction between the sulfonic acid group and the piperazine ring in its molecular structure, which can resist the influence of a small amount of acid and alkali on the pH of the solution.
In addition, this substance has mild chemical properties and has no obvious interference or damage to many biomolecules. In the study of biological macromolecules such as proteins and enzymes, it can provide a stable environment to ensure the accuracy and reliability of experimental results.
And because of its chemical stability and low toxicity, it is also favored in biomedical fields such as cell culture, which can create an environment suitable for cell growth.
In conclusion, 1,4-piperazine disodium disulfonate plays an important role in the field of biochemical and biomedical research due to its unique chemical properties, and is an indispensable tool for researchers.

1% 2C4 - Piperazinebis (Ethanesulfonic Acid), Disodium Salt is the disodium salt of 1,4 - piperazine diethylsulfonate, which is mainly used in the following fields:
In the field of biochemical research, it is often used as a buffer. In many biochemical reactions, the pH of the reaction environment has a great impact on the reaction process and results. This salt can effectively maintain the stability of the pH value of the system and avoid interference with the structure and function of biomolecules due to pH fluctuations. For example, in enzymatic reaction experiments, specific enzymes often need to have the best activity under suitable pH conditions. The disodium salt of 1,4-piperazine diethylsulfonate can create and maintain this stable acid-base environment, ensure the smooth progress of enzymatic reactions, and help researchers accurately explore the characteristics and reaction mechanisms of enzymes.
The field of cell culture is also an important place for it. Cells are extremely sensitive to the pH of their environment, and a slight deviation may affect cell growth, proliferation and even survival. As a key component of cell culture medium, this salt can precisely regulate the pH of the culture medium, create a suitable living environment for cells, and ensure the success of cell culture experiments. Whether it is conventional cell line culture or special cell culture such as stem cells, it is indispensable. In terms of protein research, in the process of protein separation, purification and crystallization, 1,4-piperazine disodium disulfonate stabilizes the pH of the solution by virtue of its buffering properties, prevents the protein from denaturing due to pH changes, and helps to obtain high-purity and high-activity proteins. It is of great significance for the in-depth study of protein structure and function.

1% 2C4 - Piperazinebis%28Ethanesulfonic + Acid% 29% 2CDisodium + Salt is the disodium salt of 1,4-piperazine diethylsulfonate, which should be stored in a cool, dry and well-ventilated place.
Looking at the characteristics of this compound, it is quite sensitive to temperature and humidity. High temperature or humid environment can easily cause its properties to change, or cause chemical reactions that damage its quality. Therefore, the temperature should be controlled between 2-8 ° C, this temperature range can ensure its chemical stability. Humidity should also be noted, and it should be maintained at a relative humidity of not more than 60% to prevent moisture decomposition.
and should be placed in a dark place away from light, because it may be sensitive to light, too long light exposure, or cause adverse reactions such as decomposition. The storage place should be separated from oxidizing agents, acids, alkalis and other chemicals to avoid chemical reactions due to improper contact and endanger safety.
And its packaging must be well sealed to ensure that there is no risk of leakage. After taking it, the packaging should be sealed immediately to avoid excessive contact with air to prevent deterioration. In this way, it should be properly stored and its quality should be guaranteed for subsequent use.

1% 2C4 - Piperazinebis%28Ethanesulfonic + Acid% 29% 2CDisodium + Salt is the disodium salt of 1,4-piperazine diethylsulfonate. There are many precautions in the use of this substance.
First, pay attention to its solubility. Although this salt has a certain solubility, it should follow the appropriate method when dissolving. If an appropriate amount of solvent is placed in a clean container first, slowly add the salt, and stir gently at the same time, do not be too hasty to prevent the local concentration from being too high and causing poor dissolution.
Second, pay attention to its pH value. It is often used as a buffer, and the pH value needs to be precisely adjusted and controlled according to specific needs. Due to the strict requirements of different reaction systems on pH value, there is a slight deviation or abnormal reaction.
Third, pay attention to its stability. Store in a dry, cool and well-ventilated place to avoid light and moisture. If not properly stored, or cause it to deteriorate, it will affect the use effect.
Fourth, safety protection should not be ignored. It is recommended to wear appropriate protective equipment during operation, such as gloves and goggles. Although it is toxic or relatively low, direct contact with the skin and eyes may still cause discomfort.
Finally, the use process should strictly follow the experimental procedures or product instructions. No matter what kind of experiment or production process it is used for, standardized operation is the key to ensure that it plays its due role, and the accuracy and safety of the results are guaranteed.

To prepare 1% 2C4 - Piperazinebis%28Ethanesulfonic Acid% 29% 2CDisodium Salt, the method is as follows:
Prepare the raw materials, need an appropriate amount of 1,4 - piperazine diethylsulfonic acid, and a solution of sodium hydroxide. Take a clean container, slowly place 1,4 - piperazine diethylsulfonic acid in it, dissolve it with an appropriate amount of water, stir well to form a clear solution. Then, while stirring, add the sodium hydroxide solution dropwise to the above solution, and measure its pH while adding. This process requires careful operation, because the reaction is quite sensitive to pH value. When the pH value reaches a certain range, that is, about a certain value, stop adding the sodium hydroxide solution dropwise. This value needs to be precisely controlled, which is related to the purity of the product. After
, the resulting solution is concentrated. Distillation under reduced pressure can be used to gradually evaporate the solvent under suitable temperature and pressure conditions, and the concentration of the solution increases. When concentrated to a certain extent, there is a sign of crystal precipitation, the solution is moved to a low temperature environment and left to cool. During this process, the crystals are slowly precipitated. When the crystals are completely precipitated, the crystals and the mother liquor are separated by filtration. The resulting crystals are washed with an appropriate amount of organic solvent to remove attached impurities. The washed crystals are placed in a dryer and dried at a suitable temperature until constant weight to obtain 1% 2C4 - Piperazinebis%28Ethanesulfonic Acid% 29% 2CDisodium Salt finished product. Throughout the preparation process, the conditions of each step, such as temperature, pH value, and reagent dosage, need to be strictly controlled in order to produce high-purity products.