(1-Oxyl-2,2,5,5-Tetramethylpyrrolidin-3-Yl) Methyl Methanethiosulfonate

(1-oxy-2,2,5,5-tetramethylpyrrolidine-3-yl) methylthionyl sulfonate, which is mostly used in the field of scientific research and experiments. It has made outstanding contributions to the investigation of protein structure and function.
In protein research, it is often used as a specific probe. The function of a protein is closely related to its structure, and cysteine residues are of great significance in protein structure stability and function. This compound can selectively react with the sulfhydryl group of cysteine residues in proteins. By changing the local chemical environment of proteins, scientists can study the relationship between protein structure and function.
For example, in the study of membrane proteins, the structure of the transmembrane region of membrane proteins is complex, and its functional mechanism is difficult to clarify. The use of (1-oxy-2,2,5,5-tetramethylpyrrolidine-3-yl) methylthiosulfonate can label specific cysteines in membrane proteins. With the help of electron paramagnetic resonance spectroscopy, the structural information of membrane proteins in different states can be obtained, thus helping to understand the principles of membrane protein material transportation, signal transduction and other functions.
Furthermore, in the study of enzyme catalytic mechanisms, cysteine in the active center of enzymes is often the key check point for catalysis. After the compound reacts with cysteine, the enzyme activity can reveal the specific process of enzyme catalytic substrate by observing the change of enzyme activity, and provide a theoretical basis for the design and development of enzyme inhibitors.

(1-oxy-2,2,5,5-tetramethylpyrrolidine-3-yl) methylthionyl sulfonate, this physical property is a special reagent in the field of organic synthesis, and its physical and chemical properties are particularly important.
Looking at its properties, it is mostly solid at room temperature, but its specific melting point is different from common organic compounds due to the interaction of special nitrogen oxide radicals and thioester groups in the molecular structure. The stable existence of nitrogen oxide radicals in the molecule gives it unique electronic properties, resulting in a relatively high melting point, about in a specific range. The specific value varies slightly according to the purity of synthesis and test conditions.
In terms of solubility, the solubility of molecules containing polar thioester groups and relatively non-polar alkyl structures varies in common organic solvents. For example, in polar organic solvents, such as dichloromethane, N, N-dimethylformamide, the solubility is good, because the polar group and the solvent can form intermolecular forces to promote dissolution; in non-polar solvents such as n-hexane, the solubility is poor, because non-polar alkyl groups are not enough to have strong interactions with non-polar solvents.
In terms of chemical stability, although nitrogen-oxygen radicals are relatively stable, in the case of strong reducing agents or high temperature conditions, the radical structure can be destroyed and chemical reactions can be initiated. At the same time, thioester groups also have certain activity, and in the presence of nucleophiles, nucleophilic substitution reactions can occur, which are used in organic synthesis or to construct new carbon-sulfur bonds, etc., showing unique chemical properties and providing the possibility for the expansion of organic synthesis reaction pathways.

(1-oxy-2,2,5,5-tetramethylpyrrolidine-3-yl) methylthiosulfonate, used in experiments as follows:
This reagent is often used for protein structure and function studies, especially to explore the role of cysteine residues in proteins. When using, first prepare a suitable buffer system, select the appropriate pH value and ionic strength according to the characteristics of the target protein, such as studying intracellular proteins, often select a buffer close to the physiological pH (about 7.4).
Dissolve an appropriate amount of the target protein in the buffer, and determine the amount of (1-oxy-2,2,5,5-tetramethylpyrrolidine-3-yl) methylthio-sulfonate according to the protein concentration and reaction requirements. Generally, the ratio of protein to reagent may vary from 1:10 to 1:100, depending on the specific situation.
Then, let the two react under suitable temperature and reaction time conditions. The temperature may be between 4 ° C and 37 ° C. Low temperature is conducive to stabilizing the protein structure, and high temperature can speed up the reaction rate. The reaction time ranges from a few minutes to several hours, and experimental optimization is also required. After the reaction is completed, gel filtration, dialysis, etc. can be used to terminate the reaction and remove unreacted reagents, so as to obtain the modified protein for subsequent analysis, such as activity detection, structural determination, etc. This method can provide insight into the relationship between protein structure and function.

(1-oxy-2,2,5,5-tetramethylpyrrolidine-3-yl) methylthionyl sulfonate, which is a rather special chemical substance. Its storage conditions need to be cautious.
Because of its specific chemical properties, it is highly susceptible to external factors. Generally speaking, it needs to be stored in a low temperature environment to prevent chemical reactions and deterioration. The ideal storage temperature is between -20 ° C and -80 ° C. Such a low temperature environment can effectively slow down the movement of molecules and reduce the possibility of them reacting with surrounding substances.
At the same time, the substance is extremely sensitive to humidity, and moisture can easily cause hydrolysis or other adverse reactions. Therefore, it should be stored in a dry place, preferably in a dryer, and a desiccant should be placed in the dryer to ensure that the internal environment is dry.
In addition, light may also affect its stability. In order to avoid photochemical reactions such as photolysis, it needs to be placed in a dark container, such as a brown glass bottle, which can effectively block light and maintain its chemical stability. Only by following these strict storage conditions can the quality and characteristics of (1-oxy-2,2,5,5-tetramethylpyrrolidine-3-yl) methylthio-sulfonate be guaranteed to the greatest extent for scientific research or other purposes.

(1-oxy-2,2,5,5-tetramethylpyrrolidine-3-yl) methylthionyl sulfonate. The risks and precautions for its dissolution need to be analyzed in detail.
When dissolving this chemical, the first thing to pay attention to is its chemical compatibility with the solvent used. If you use an inappropriate solvent rashly, or cause a violent chemical reaction, the properties of the solution will change in the light case, and in the serious case, it will cause extreme danger such as explosion. And because its structure contains specific groups, some common organic solvents meet it or cause unexpected changes.
Furthermore, the dissolution operation must be carried out in a well-ventilated environment. Because it may evaporate harmful gases, if the ventilation is not smooth and the harmful gases accumulate, the operator will seriously damage health after inhalation, and symptoms such as respiratory irritation and dizziness may occur.
In addition, the temperature control of the dissolution process is also crucial. If the temperature is too high, or it accelerates its decomposition, causing chemical properties to change and the product to be impure; if the temperature is too low, the dissolution rate will be slow, which will affect the experimental process. Therefore, the temperature needs to be precisely regulated according to its physical and chemical properties.
And when dissolving, the stirring rate cannot be ignored. If the rate is too fast, or local overheating or overcooling will affect the dissolution uniformity; if the rate is too slow, the dissolution efficiency will be low.
In conclusion, the dissolution of this substance requires careful consideration of many factors and careful action to ensure safety and the smooth progress of the experiment.