What is the main use of 2,2 '-Azino-Bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) Diammonium Salt?
2% 2C2% 27-azobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, its Chinese abbreviation is ABTS diammonium salt. This substance has a wide range of uses and is crucial in the field of biochemical research.
First, it is often used as a redox indicator. In many redox reactions, it can keenly display color changes, just like an ancient war beacon, with a direct view of the color, indicating the process and degree of the reaction. Due to the redox potential change of the reaction system, ABTS diammonium salt will show a significant color change, allowing the experimenter to accurately understand the trend of the reaction, grasp the key nodes, and guide the direction in analytical chemistry experiments, like a navigational compass.
Second, it has made outstanding contributions to the field of enzyme activity detection. Many enzymatic reactions involve redox processes, and ABTS diammonium salt can be used as a substrate or indicator. For example, horseradish peroxidase detection, ABTS diammonium salt is enzyme-catalyzed oxidation, showing a unique color. According to the color depth, the experimenter can accurately determine the enzyme activity. Just like a scale for measuring the height of martial arts, it provides a powerful tool for the evaluation of enzyme activity in biochemical research.
Third, it is also useful in immunoassay. In immunoassay techniques such as ELISA, ABTS diammonium salt is often used as a color developer. After specific binding of antigens and antibodies, ABTS diammonium salt is catalyzed by a labeling enzyme to produce a color reaction, and qualitative and quantitative analysis of the target substance in the sample is achieved according to the color depth. It is of great significance in the fields of disease diagnosis, food safety testing, etc.
What are the chemical properties of 2,2 '-Azino-Bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) Diammonium Salt
2% 2C2% 27 - Azino - Bis% 283 - Ethylbenzothiazoline - 6 - Sulfonic Acid% 29 Diammonium Salt, Chinese name is often 2,2 '-biazo-bis (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt, referred to as ABTS. This substance has unique chemical properties and is an important reagent commonly used in scientific research.
Its properties are soluble in water and can form a stable solution, which is colorless to slightly yellow, and gradually changes color when exposed to light or heat. In redox reactions, ABTS often plays a key role because it can be oxidized by specific oxidants to generate cationic radicals with strong absorption peaks. This property is widely used in the spectrophotometric detection of many substances.
And because of its good water solubility and stability, ABTS is an indispensable tool in the field of biochemical analysis, such as enzyme activity determination and antioxidant capacity evaluation. The reaction conditions it participates in are mild and have little effect on the activity of biomolecules, so it can accurately detect the redox process in biological systems. And when it interacts with peroxidase, it can exhibit efficient catalytic properties, making the reaction fast and sensitive, providing strong support for the study of many complex biochemical reactions.
What are the usage and precautions of 2,2 '-Azino-Bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) Diammonium Salt in the experiment?
2% 2C2% 27 - Azino - Bis% 283 - Ethylbenzothiazoline - 6 - Sulfonic Acid% 29 Diammonium Salt, which is ABTS diammonium salt, is often used as a chromogenic substrate in experiments to detect peroxidase and other activities. The method of use and precautions are as follows:
- * How to use **: Usually, ABTS is dissolved in a suitable solvent such as water to prepare a certain concentration of stock solution, generally several milligrams per milliliter. During the experiment, according to specific needs, the stock solution is mixed with buffer solution, enzyme solution, etc. in proportion. For example, to detect peroxidase, the substrate ABTS solution, hydrogen peroxide, and the enzyme solution to be tested are added to the buffer system in sequence, and the reaction is carried out at a suitable temperature. The enzyme catalyzes the oxidation of ABTS by hydrogen peroxide to change the color of the solution. A spectrophotometer is used to measure the absorbance at a specific wavelength (such as 414nm or 652nm), and the reaction process and enzyme activity are monitored by the change of absorbance.
- ** Precautions **: First, the stability of ABTS after dissolution is limited. The stock solution needs to be prepared now or stored at low temperature for a short period of time to avoid its deterioration affecting the experimental results due to long-term placement. Second, the pH value of the reaction system is very important. Different enzymatic reactions have different pH requirements. It is necessary to strictly control the pH of the buffer to ensure that the enzyme activity and ABTS reaction are normal. Third, the reagents used in the experiment should be pure to avoid impurities interfering with the reaction. Fourth, ABTS is irritating to a certain extent, and appropriate protective equipment such as gloves and goggles should be worn during operation to prevent contact with the skin and eyes.
What are the storage conditions for 2,2 '-Azino-Bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) Diammonium Salt?
2% 2C2% 27 - Azino - Bis% 283 - Ethylbenzothiazoline - 6 - Sulfonic Acid% 29 Diammonium Salt is 2,2 '-azo-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, often referred to as ABTS. The storage conditions are as follows:
This compound should be stored in a cool and dry place, away from direct sunlight. Its chemical properties may be affected by light, causing it to decompose or deteriorate. Storage in the dark can keep its chemical structure stable. The temperature should be controlled at 2-8 ° C. In this temperature range, ABTS can maintain good stability, slow down the possible chemical reaction rate, and reduce the risk of degradation.
In addition, when storing, make sure that the container is well sealed. Because of its hygroscopicity, if exposed to air, it is easy to absorb moisture, which affects the purity and performance. Sealed storage can prevent moisture from invading and maintain its dry state.
Furthermore, it should be kept away from strong oxidizing agents and reducing agents. ABTS can chemically react with such substances, causing them to lose their original characteristics and functions. When storing, it should be placed separately from such chemicals to ensure a safe storage environment to avoid dangerous reactions. Strictly follow the above storage conditions to keep 2,2 '-biazo-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt stable for a long time for experimental or production needs.
What are the relevant detection methods for 2,2 '-Azino-Bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) Diammonium Salt?
2% 2C2% 27 - Azino - Bis% 283 - Ethylbenzothiazoline - 6 - Sulfonic Acid% 29 Diammonium Salt is ABTS diammonium salt. The relevant detection methods are as follows:
Colorimetry
This is a commonly used method for detecting ABTS diammonium salts. ABTS generates stable blue-green cationic radicals after oxidation. Peroxidase and other catalysts are used to oxidize ABTS in the presence of suitable substrates. Then, with the help of spectrophotometer, the absorbance is measured at a specific wavelength (usually 414nm, 650nm, etc.). The absorbance is closely related to the concentration of ABTS oxidation products. By comparing it with the standard curve, the content of related substances in the sample can be known. For example, when detecting antioxidant capacity, antioxidants inhibit the generation of ABTS cationic radicals, and the activity of antioxidants can be evaluated according to the change of absorbance.
Electrochemical method
uses the electrochemical reaction of ABTS on the electrode surface to detect. An electrochemical cell is constructed by the working electrode, the reference electrode and the counter electrode, and the sample containing ABTS is placed in it. When a suitable potential is applied, the ABTS will oxidize or reduce on the electrode surface, and the generated current signal is related to the concentration of ABTS. Qualitative and quantitative detection of ABTS diammonium salt can be realized by analyzing electrochemical signals such as current-potential curves. This method has high sensitivity and fast detection speed.
Fluorescence method
In some cases, ABTS diammonium salt or its reaction products will have fluorescence characteristics. Fluorescence spectrometers can be used to detect the fluorescence intensity emitted by the sample at a specific excitation wavelength. The fluorescence intensity is correspondingly related to the concentration of ABTS, so as to achieve the detection purpose. Compared with colorimetry, fluorescence method is often more sensitive and selective, and has obvious advantages in the detection of trace ABTS.