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2,2 '-Azino-Di (3-Ethyl-Benzthiazoline Sulphonic Acid (6)), what is the chemical structure of Ammonium Salt
2% 2C2% 27 -azabis (3-ethylbenzothiazoline-6-sulfonic acid), ammonium salt, its chemical structure is as follows: The compound is composed of two benzothiazoline rings connected by nitrogen and nitrogen double bonds. The 3-position of the benzothiazoline ring is connected with ethyl group, and the 6-position is connected with sulfonic acid group, and the whole exists in the form of ammonium salt. Among them, the benzothiazoline ring is a heterocyclic structure containing nitrogen and sulfur. The nitrogen-nitrogen double bond endows the molecule with certain conjugation characteristics. The sulfonic acid group makes the compound have good water solubility, and the ammonium salt form further enhances its solubility and ionic properties in water. This structure makes the compound exhibit unique properties and application value in many fields, such as biochemical analysis, optoelectronics, etc.
2,2 '-Azino-Di (3-Ethyl-Benzthiazoline Sulphonic Acid (6)), what are the main uses of Ammonium Salt
2% 2C2% 27 - Azino - Di% 283 - Ethyl - Benzthiazoline Sulphonic Acid% 286% 29% 29, Ammonium Salt is 2,2 '-azo-bis- (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt, often referred to as ABTS ammonium salt. This substance has a wide range of uses and is described in detail as follows:
In the field of biochemical testing, it is a peroxidase substrate. When coexisted with peroxidase and hydrogen peroxide, it will develop a color reaction to produce a stable blue-green product. This property is commonly used in enzyme-linked immunosorbent assays (ELISA) to detect changes in absorbance and to sensitively determine peroxidase activity or specific analyte content. Taking the detection of a certain disease marker as an example, the marker antibody is fixed on the microporous plate, the marker-containing sample and enzyme-labeled secondary antibody are added, and then the ABTS substrate is added. The enzyme catalyzes the color development of the substrate. According to the color depth, the marker content can be determined, which can help disease diagnosis and monitoring.
In the food industry, it can be used as an antioxidant capacity evaluation reagent. Because of its cationic free radical stability blue characteristics, antioxidants can be reduced to make the color lighter when present. By measuring the change of absorbance by colorimetry, the antioxidant capacity of food, health products or plant extracts can be evaluated, providing key data for product development and quality control, assisting in the selection of high-quality antioxidant raw materials, and improving the antioxidant efficacy of products.
In terms of water quality monitoring, it can detect oxidizing substances in water. Some oxidizing pollutants can oxidize ABTS, causing its color or absorbance to change. According to this principle, the construction of detection methods to quickly determine the concentration of oxidizing pollutants in water is of great significance for evaluating water quality safety and ensuring drinking water hygiene. It can detect abnormal water quality in time and take measures to deal with it to protect public health.
2,2 '-Azino-Di (3-Ethyl-Benzthiazoline Sulphonic Acid (6)), Ammonium Salt What are the precautions during use
2% 2C2% 27 - Azino - Di% 283 - Ethyl - Benzthiazoline Sulphonic Acid% 286% 29% 29, Ammonium Salt is 2,2 '-azo-bis- (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, often referred to as ABTS. During use, there are many precautions to be paid attention to.
First, it concerns storage conditions. This material should be placed in a cool, dry and well-ventilated place, away from heat sources and open flames. Due to its chemical properties or environmental factors, improper storage or deterioration can affect the use effect. If stored in a humid environment, it is vulnerable to moisture hydrolysis and changes its chemical structure and activity.
Second, it involves operating specifications. When operating, it is necessary to strictly follow relevant safety procedures and wear appropriate protective equipment, such as gloves, goggles, etc. Because of its irritating effect on the skin and eyes, once inadvertently contacted, it should be rinsed with a lot of water immediately, and seek medical attention in time as appropriate. During the weighing and preparation process, it is necessary to operate accurately to ensure the accuracy of experimental or production results. If the weighing error is large, the concentration of ABTS in the reaction system will be abnormal, which will affect the reaction process and final product.
Third, pay attention to its compatibility with other substances. Before mixing with other chemical reagents, be sure to understand their interactions. Some substances may react chemically with ABTS, reducing its activity or producing adverse side effects. For example, specific strong oxidizing or reducing agents may cause the structure of ABTS to change and fail to achieve the intended experimental purpose.
Fourth, pay attention to the use period. ABTS has a certain shelf life. After the expiration date, its performance may decline. Using expired reagents not only makes it difficult to obtain ideal experimental data, but also may mislead research conclusions. Therefore, check the production date and shelf life before use to ensure that it is used within the valid time.
2,2 '-Azino-Di (3-Ethyl-Benzthiazoline Sulphonic Acid (6)), Ammonium Salt What are the storage conditions?
2% 2C2% 27 -azido-bis (3 -ethyl-benzothiazoline sulfonic acid (6)) ammonium salt, this substance should be stored in a cool, dry and well-ventilated place. It is afraid of light and heat, so it should avoid direct light, and the ambient temperature should not be too high. If the temperature is too high, it may cause its chemical properties to change, reducing its quality and utility.
Furthermore, it needs to be stored separately from oxidizing substances, acids and bases. Because of oxidizing substances or causing oxidation reactions, acids and bases may chemically react with them, thereby damaging their structure and properties.
When storing, the container must be tightly sealed to prevent it from coming into contact with water vapor, oxygen and other components in the air. Water vapor in the air may deliquesce it, and oxygen may oxidize it.
In addition, the storage site should be equipped with suitable fire equipment and leakage emergency treatment equipment. If there is a leak, it can be properly disposed of in time to avoid the expansion of harm. And it is necessary to arrange a special person to regularly inspect the storage situation to pay attention to whether there are abnormal phenomena such as package damage and deterioration. If so, it is necessary to ensure the safe storage of this substance and maintain its chemical stability.
2,2 '-Azino-Di (3-Ethyl-Benzthiazoline Sulphonic Acid (6)), How stable is Ammonium Salt?
2% 2C2% 27 -azido-bis (3 -ethyl-benzothiazoline sulfonic acid (6)) ammonium salt, its stability is very important. Under suitable conditions, this compound can maintain a relatively stable state.
If the ambient temperature is too high, it may increase the chemical bond energy in its structure, which will enhance the molecular activity, impair the stability, or initiate reactions such as decomposition. Similarly, excessive humidity may also affect its stability, and moisture or interact with the compound, altering its chemical properties.
Light is also a key factor affecting its stability. If exposed to strong light for a long time, especially light with sufficient energy, or prompts the molecule to undergo photochemical reactions, its structure will change and its stability will decrease.
When storing this compound, it is necessary to choose a cool, dry and dark place and seal it to maintain its stability to the greatest extent, reduce the adverse effects of external factors on its chemical structure and properties, and ensure that it can exhibit the expected characteristics and efficacy when used.