2-[4-(2-Hydroxyethyl)-1-Piperazine]Ethanesulfonic Acid Sodium Salt

2-% 5B4-% 282 - Hydroxyethyl% 29-1 - Piperazine%5DEthanesulfonic Acid Sodium Salt, which is 2- (4- (2-hydroxyethyl) -1-piperazinyl) ethanesulfonate sodium salt, is often abbreviated as HEPES sodium salt. This substance is widely used in many experiments.
In cell culture experiments, HEPES sodium salt plays a key role. Cell culture requires a stable pH environment, and HEPES sodium salt can maintain a stable pH in the culture medium due to its excellent buffering ability. Cell physiological functions can operate normally within a suitable pH range. If the pH fluctuates too much, it will affect cell growth, metabolism and even cause cell death. HEPES sodium salt can effectively resist pH changes caused by external factors, create a stable living environment for cells, and enable cell culture experiments to proceed smoothly.
This substance is also commonly used in biochemical experiments. The study of biological macromolecules such as proteins and nucleic acids requires a specific buffer system to maintain their structure and function stability. The HEPES sodium salt buffer system can simulate the environment in organisms to prevent structural damage or loss of function of biological macromolecules due to environmental changes. In experiments such as protein purification and enzyme activity determination, HEPES sodium salt is required to build a suitable buffer environment to ensure accurate and reliable experimental results.
Furthermore, HEPES sodium salt is also very important in drug development experiments. Drug development often involves in vitro cell experiments and animal experiments, regardless of the stage, appropriate experimental conditions are required. HEPES sodium salt can adjust the pH of the experimental system, ensure the stability and activity of the drug, help to accurately evaluate the efficacy and safety of the drug, and provide key support for drug development.
In summary, 2- (4- (2-hydroxyethyl) -1-piperazinyl) ethanesulfonate sodium salt plays an indispensable role in many experimental fields such as cell culture, biochemistry and drug development, providing a strong guarantee for the smooth development of scientific research.

2-% [4- (2 - Hydroxyethyl) -1 - Piperazine] Ethanesulfonic Acid Sodium Salt, commonly known as HEPES sodium salt, is widely used in biochemical experiments and other fields. Its purity requirements are not fixed and often vary depending on the specific use scenario.
In the field of cell culture, cells are extremely sensitive to the culture environment, and the purity of HEPES sodium salt needs to be above 99%. Such high purity can ensure a stable cell culture environment and avoid impurities interfering with cell growth, metabolism and the accuracy of experimental results. If the purity is insufficient, impurities may affect cell osmotic pressure, hinder normal cell proliferation, and even cause cell lesions and death, seriously interfering with the reliability and repeatability of the experiment.
In the enzymatic reaction, HEPES sodium salt is used as a buffer, and the purity requirement is usually about 98%. The activity of the enzyme is easily affected by the environment. Although its tolerance to impurities is slightly higher than that of cell culture, too many impurities will still combine with the enzyme non-specifically, changing the spatial conformation of the enzyme, reducing or even inactivating the enzyme activity, interfering with the reaction process and result analysis.
In the general buffer system configuration, the purity requirement may be slightly reduced, about 95%. Due to the relatively low sensitivity to impurities in this scene, its buffering ability is mainly used to maintain the pH stability of the system, and the tolerance to potential subtle effects caused by impurities is high.
In summary, the purity requirements of 2-% [4- (2-Hydroxyethyl) -1-Piperazine] Ethanesulfonic Acid Sodium Salt vary depending on the application scenario, ranging from 95% to more than 99%. The use needs to be strictly selected according to the specific experimental purposes and requirements.

2-% 5B4-% 282 - Hydroxyethyl% 29-1 - Piperazine%5DEthanesulfonic Acid Sodium Salt, which is 2- (4- (2-hydroxyethyl) -1-piperazinyl) ethanesulfonate sodium salt, often referred to as HEPES sodium salt. The proper storage of this product is related to its quality and effectiveness. It is necessary to pay attention to the following numbers.
Its nature likes cool but is afraid of heat. It should be stored in a cool place, and the temperature should not exceed 25 ° C. If it is in a high temperature environment, its molecular structure may change and its chemical activity will also be affected.
And this product is resistant to moisture. It must be placed in a dry place. The humidity should be controlled at 40% - 60%. If the moisture is too heavy, or it causes deliquescence, it is not only inconvenient to use, but also easy to cause chemical reactions and damage its purity.
Furthermore, it must be protected from light. Light or cause its photochemical reaction to cause deterioration of the ingredients. Therefore, it should be stored in an opaque container or stored in a dark place.
When using it, also be careful. Use clean and dry utensils to prevent impurities from mixing, and seal the container after use to ensure that it is not disturbed by the external environment. In this way, it is necessary to properly store 2- (4- (2-hydroxyethyl) -1-piperazinyl) ethanesulfonate sodium salt, so that its performance is constant, so as to prepare for scientific research, experiments and other needs.

2-% 5B4-% 282 - Hydroxyethyl% 29-1 - Piperazine%5DEthanesulfonic Acid Sodium Salt, which is 2- [4- (2-hydroxyethyl) -1-piperazine] ethane sulfonate sodium salt, is often abbreviated as HEPES-Na. The solubility of this substance is considerable.
Looking at its molecular structure, it contains hydrophilic groups such as hydroxy (-OH) and sulfonic acid (-SO 🥰 Na), both of which are strongly hydrophilic. Hydroxyl groups can be closely linked to water molecules by hydrogen bonds, and ions generated by the ionization of sulfonic acid groups can also easily interact with water molecules, greatly enhancing their solubility in water.
In the experimental routine, an appropriate amount of 2- [4- (2-hydroxyethyl) -1-piperazine] ethane sulfonate sodium salt is placed in water, slightly stirred or assisted by ultrasound, and it dissolves rapidly to form a clear and transparent solution. Under normal circumstances, it can be easily dissolved in water at room temperature, and the solubility can reach tens of grams per 100 milliliters of water. Not only in water, but also in some polar organic solvents, such as methanol and ethanol, it has some solubility, but the solubility may be inferior to water. Although methanol and ethanol are polar solvents, their degree of polarity is slightly lower than that of water, and the interaction with the solute is slightly weaker. Overall, 2- [4- (2-hydroxyethyl) -1-piperazine] ethane sulfonate sodium salt exhibits good solubility due to its own structural characteristics, which lays the foundation for its wide use in many experimental and industrial applications.

2-% 5B4-% 282 - Hydroxyethyl% 29-1 - Piperazine%5DEthanesulfonic Acid Sodium Salt, the Chinese name is often 2- (4- (2-hydroxyethyl) -1-piperazinyl) ethanesulfonate sodium salt, generally referred to as HEPES sodium salt.
This substance is commonly used in biochemical experiments and cell culture. Its properties are mostly used to regulate and maintain the pH of the system, create a stable acid-base environment for cells, and help cells grow and metabolize normally.
As for its biological toxicity, it is difficult to simply assert. Covered within the appropriate concentration range, it has little effect on most cells and biological systems and does not show obvious toxicity. If the cell culture is added according to the conventional dosage, cell growth, proliferation and other activities can be carried out normally.
However, if the concentration is too high or used improperly, it may also cause adverse effects. At high concentrations, it may interfere with the balance of cell osmotic pressure, affect physiological processes such as cell material transportation, and even cause toxicity to cells, resulting in cell growth inhibition, morphological changes and even apoptosis.
And different cell types and biological systems also have differences in their tolerance. Some sensitive cells are more sensitive to changes in their concentration, and low concentration changes may also lead to changes in physiological responses. Therefore, considering its biological toxicity, when considering various factors, such as the concentration of use, the time of action, and the object of action, it cannot be generalized.