2-(4-Morpholino) Ethane Sulfonic Acid

2-%284-Morpholino%29+Ethane+Sulfonic+Acid, the Chinese name 2- (4-alkyl) ethanesulfonic acid, is often used for MES. Its chemical properties are very special, let me say.
MES is a compound with chemical properties, and its molecular properties, the sulfonic acid group (-SO-H), and the nitrogen-containing alkanolinic acid are derived from the ethane-based phase. This special property gives it its general properties.
In terms of acidity, MES is a good biological performance. Its pKa value is around 6.15, and it can exhibit excellent performance at a pH of 5.5 - 6.7. In the case of polybiotic molecular biology, this pH is very important. For example, some enzyme reactions need to be performed at this specific pH. MES can maintain a certain acid environment and ensure the activity of enzymes and the normal operation of reactions.
In terms of solubility, MES is easily soluble in water, which makes it convenient to prepare aqueous solutions. It can quickly dissolve to form a homogeneous solution, providing a reliable reaction medium.
In terms of qualitative aspects, MES is qualitative under normal temperature, acidity, and general conditions. However, in case of oxidation or oxidation, it may be affected. For the use of MES in some systems involving oxidizing proto-antibodies, it is necessary to pay attention to this, in order to prevent its antibodies from causing unnecessary chemical reactions and affecting the results.
Therefore, 2- (4-alkyl) ethanesulfonic acid, by virtue of its own chemical properties, plays an indispensable role in many fields such as biochemistry and molecular biology, helping researchers to develop their research.

2-%284-Morpholino%29+Ethane+Sulfonic+Acid is 2- (4-morpholinyl) ethanesulfonic acid, often referred to as MES. Its common uses are quite extensive.
In the field of biochemistry and molecular biology experiments, MES is often used as a buffer. Because it has the effect of stabilizing the pH value of the solution, it can effectively resist pH changes within a specific pH range (generally 5.5-6.7). For example, in protein and nucleic acid research experiments, maintaining a suitable and stable pH environment is of great significance to ensure the structure and activity of biological macromolecules, and MES buffers are often selected.
MES also plays a key role in cell culture. The growth and metabolism of cells are extremely sensitive to the pH of the environment in which they are located. MES can help create a stable microenvironment to meet the needs of cell growth and promote normal cell proliferation and the execution of physiological functions.
In some enzymatic reaction systems, MES is also frequently used. The catalytic activity of many enzymes depends on specific pH conditions. MES buffer can ensure that the reaction is carried out in the pH range with the best enzyme activity, improve the reaction efficiency and stability, and make the relevant research and production process smoother.
In addition, in some drug development and preparation production processes, pH needs to be precisely controlled to ensure the stability and effectiveness of drugs. MES can effectively adjust and maintain the appropriate pH, so it also has a certain degree of application in it.

To prepare 2 - (4 - morpholinyl) ethanesulfonic acid, the method is as follows:
First take an appropriate amount of morpholine, place it in a reactor, use water as a solvent, and stir well. This is because water is a common and suitable solvent, which can fully mix the reactants and facilitate the reaction.
Then, slowly add 1,2 - dichloroethane, and control the reaction temperature within a certain range, such as 50 - 70 ° C. This temperature range has been verified by many experiments. In this range, the reaction rate and product purity are ideal. The nucleophilic substitution reaction occurs between the two. The nitrogen atom in the morpholine is rich in electrons and attacks the chlorine atom in 1,2 - dichloroethane. The chlorine atom leaves to form an intermediate product.
During the reaction process, the reaction progress can be closely monitored by thin layer chromatography or high performance liquid chromatography. When the reaction is basically completed, the reaction solution is cooled to room temperature.
Next, an appropriate amount of sodium hydroxide solution is added to the reaction solution, and the pH is adjusted to the alkaline range, about 9-10. This operation is designed to further convert the intermediate product and promote the formation of sulfonic acid groups. After
, the reaction mixture is extracted, and the organic phases are combined with an organic solvent such as ethyl acetate. The purpose of extraction is to separate the target product and improve its purity.
The organic phase is then distilled to remove the organic solvent and obtain a crude product.
Finally, the crude product is recrystallized and a suitable solvent is selected, such as ethanol-water mixed solvent. After recrystallization, the product can be further purified and finally obtained with high purity of 2- (4-morpholino) ethanesulfonic acid.

2-%284-Morpholino%29+Ethane+Sulfonic+Acid is 2- (4-morpholine) ethanesulfonic acid, often referred to as MES. This is a commonly used buffer in biochemical research. When storing, pay attention to the following numbers:
First, pay attention to the storage temperature. MES should be stored in a cool place. If the temperature is too high, it is easy to change its chemical properties. Generally speaking, it is better to refrigerate at 2-8 ° C. If it is in a high temperature environment, MES may accelerate decomposition and affect its buffering performance. Therefore, it is necessary to choose a storage place with a suitable temperature, such as the refrigerated layer of the refrigerator.
Second, pay attention to humidity control. Because it is hygroscopic, it should be stored in a dry place, away from water sources and heavy moisture after being damp or affecting quality. It can be placed in a dryer with a built-in desiccant to maintain a dry environment and prevent MES from agglomerating or deteriorating due to moisture absorption.
Third, it needs to be stored away from light. Light or chemical reactions are unfavorable to its stability. It should be stored in an opaque container, such as a brown bottle, to reduce the influence of light on it and ensure stable chemical properties.
Fourth, to prevent pollution. During storage, ensure that the container is well sealed to avoid impurities and microorganisms from mixing. If the container is poorly sealed, external substances are easy to enter and contaminate the MES, making it impossible to use it for experiments. During the access process, pay attention to the operating specifications and use clean appliances to prevent cross-contamination.

2-%284-Morpholino%29Ethane Sulfonic Acid is 2- (4-morpholino) ethanesulfonic acid, often referred to as MES. This is a commonly used amphoteric ion buffer in biochemical research. When using it, pay attention to many safety matters, as detailed below:
One is a protective measure. Because it may cause irritation to the eyes, skin and respiratory tract, it is necessary to wear appropriate protective equipment when operating. When involving powdered MES, wear a dust mask to prevent inhalation of dust; at the same time, safety glasses, lab clothes and gloves are also indispensable to avoid direct contact with the body.
The second is related to storage conditions. MES should be stored in a cool, dry and well-ventilated place, away from fire and heat sources. It needs to be stored separately from oxidants, acids, alkalis, etc., and must not be mixed to prevent dangerous chemical reactions. The temperature and humidity of the storage environment should also be strictly controlled. Generally, the temperature should be maintained at 15 ° C -30 ° C, and the relative humidity should not be higher than 75%.
The third is emergency treatment. If you accidentally come into contact with your eyes, you should immediately rinse with a large amount of flowing water for no less than 15 minutes, and seek medical treatment in time; if the skin comes into contact with MES, you need to quickly remove contaminated clothes and rinse the skin thoroughly with a large amount of soapy water and water; once inhaled, you should move to a fresh place as soon as possible to keep the respiratory tract unobstructed. If breathing difficulties, you need to give oxygen. If breathing stops, you should immediately perform artificial respiration and send it to the hospital for first aid; in case of ingestion, do not induce vomiting. You should immediately drink an appropriate amount of warm water and seek medical attention.
The fourth is disposal. Discarded MES and its packaging must not be discarded at will. It needs to be handed over to a waste treatment institution with corresponding qualifications for disposal in accordance with local regulations. For unused MES, do not return to the original container to prevent contamination of the entire bottle of reagents.
Only during the operation of 2- (4-morpholinyl) ethanesulfonic acid, the above safety precautions are strictly followed to ensure the safety of the experimenter and the safety of the experimental environment.