What are the main uses of 2- (4-Morpholino) Ethanesulphonic Acid?
2-%284-Morpholino%29Ethanesulphonic + Acid is 2- (4-morpholinyl) ethanesulfonic acid, often referred to as MES. Its main uses are as follows:
MES is a biological buffer and has a wide range of uses in biochemical research. In the field of cell culture, it can create and maintain a stable pH environment. The normal growth and metabolism of cells are highly dependent on an appropriate pH. MES can prevent large fluctuations in pH caused by factors such as the accumulation of cell metabolites, provide a stable living space for cells, and ensure the accuracy and reproducibility of cell experiments.
MES also plays a key role in the study of enzyme activity. Many enzymes only exhibit the best activity within a specific pH range. The MES buffer system can precisely regulate the pH of the reaction system, which helps researchers to explore the activity changes of enzymes under different conditions, analyze the enzymatic reaction mechanism, and then understand the complex biochemical processes in life.
In protein crystallization experiments, MES is indispensable. Protein crystallization is extremely sensitive to environmental conditions, and pH is one of the key factors. MES can effectively adjust the pH of the crystallization solution, help protein molecules arrange in an orderly manner, and promote the formation of crystals, laying the foundation for in-depth research on the structure and function of proteins.
In addition, in electrophoresis experiments, MES buffer can ensure the stability of pH during electrophoresis, so that biological macromolecules (such as nucleic acids, proteins, etc.) can be effectively separated in the electric field according to their own charge and size, and improve the resolution and reliability of experimental results. In short, 2- (4-morpholino) ethanesulfonic acid is an indispensable and important reagent in many fields of life science due to its unique buffering properties.
2- (4-Morpholino) Ethanesulphonic Acid Storage
2-%284-Morpholino%29Ethanesulphonic Acid is 2- (4-morpholine) ethanesulfonic acid, often referred to as MES, which is a buffer commonly used in biochemical experiments. When storing this agent, when talking about the ancient method, you need to pay attention to the following things.
First, moisture avoidance is the first thing. MES is very easy to absorb moisture. If it is placed in a humid place, its water content will increase, resulting in a decrease in purity and a change in buffering performance. Therefore, it needs to be stored in a dry place. It can be stored in a sealed container like the ancients, and then placed next to a lime tank and other moisture-absorbing objects to keep it dry.
Second, temperature control is crucial. High temperature is easy to chemically change MES, causing its properties to change. Therefore, it should be stored in a cool place, generally 2-8 ° C. This temperature range can effectively delay its possible deterioration.
Third, it is necessary to prevent it from mixing with impurities. MES should not coexist with strong oxidants, strong bases, etc., otherwise it is easy to cause chemical reactions and damage its quality. Storage places should be away from such substances, and the receptacles used should be clean, and no other chemical substances should be left.
Fourth, the label should be clear and cannot be ignored. The name, purity, production date and other information should be detailed on the container containing MES, so that it can be used and inspected in the future. If the label is unclear, it is easy to misuse and cause experimental deviation.
In short, proper storage of MES requires attention to moisture resistance, temperature control, anti-impurities, and clear labels, so as to ensure that it can play a good buffering effect in experiments.
What is the standard of purity for 2- (4-Morpholino) Ethanesulphonic Acid?
2-%284-Morpholino%29Ethanesulphonic Acid is 2- (4-morpholinyl) ethanesulfonic acid, often referred to as MES. Its purity standards vary in different application scenarios, as detailed below.
In biochemical and molecular biology experiments, this is a commonly used buffer, and the purity needs to reach a very high level. Generally speaking, the purity standard of high-purity MES products is more than 99%. Such high purity is designed to ensure the stability and reliability of the experimental system. If it contains impurities, or interferes with enzyme activity, affects the stability of nucleic acid, and then causes the experimental results to be biased. For example, in protein crystallization experiments, even small impurities may change the microenvironment around the protein and hinder the crystallization process.
In drug research and development and some pharmaceutical fields with strict purity requirements, MES purity standards may be stricter. In addition to purity above 99%, the content of specific impurities is also strictly limited. If there are heavy metal impurities and organic impurities that may affect the safety and effectiveness of the drug, they need to be controlled at a very low level. This is because the drug is directly related to human health, and any impurities may cause adverse reactions.
In industrial production and other scenarios with relatively low purity requirements, MES purity may be moderately relaxed. However, it usually needs to reach more than 95% to meet basic production needs and ensure product quality stability. In short, the purity standard of 2- (4-morpholinyl) ethanesulfonic acid depends on the specific application, from more than 95% in industrial production to more than 99% or even more stringent requirements in the fields of biochemistry and pharmaceuticals, all of which are designed to meet the requirements of product quality and performance in different scenarios.
What are the possible risks of 2- (4-Morpholino) Ethanesulphonic Acid?
2-%284-Morpholino%29Ethanesulphonic Acid is 2- (4-morpholine) ethanesulfonic acid, commonly known as MES, may have the following risks during use:
First, the risk of exposure. If it is accidentally in contact with the skin, or causes skin irritation, causing skin redness, swelling, itching and other discomfort, as if the skin is disturbed by evil and cannot be peaceful; if it touches the eyes, it is even more harmful, will cause serious irritation to the eyes, and there is a risk of damage to vision. It is like a pearl dusting, which is worrying.
Second, the risk of inhalation. When used, if its dust or volatiles are inhaled, it will irritate the respiratory tract, making people cough and asthma, as if the airway is blocked by foreign bodies, and the breathing is not smooth. In severe cases, it may cause respiratory inflammation and affect the normal operation of the respiratory system.
Third, the risk of accidental ingestion. If accidentally ingested, MES enters the body, or stimulates the gastrointestinal tract, causing nausea, vomiting, abdominal pain and other symptoms, just like the gastrointestinal tract suffers from civil disturbance, disturbing and disturbing, causing damage to physical health.
Fourth, environmental risks. If not handled properly after use, MES will flow into the environment, or affect the ecological environment such as water bodies and soils, destroying the ecological balance, like a stone thrown into a calm lake, causing layers of bad ripples.
Therefore, when using 2- (4-morpholine) ethanesulfonic acid, it is necessary to strictly follow the safety operating procedures, take protective measures, and beware of the above risks to ensure personal safety and environmental stability.
2- (4-Morpholino) Ethanesulphonic Acid
2 - (4 - morpholino) ethanesulfonic acid, often referred to as MES, is a commonly used buffer in biochemical research. Its common synthesis methods are as follows:
Starting material, 4 - morpholino acetonitrile and concentrated sulfuric acid can be selected. First, 4 - morpholino acetonitrile is slowly injected into concentrated sulfuric acid. This step needs to be handled with caution because of its violent reaction. Maintain a certain temperature so that the two can fully react to produce 2 - (4 - morpholino) ethanesulfonamide.
Then, the resulting sulfonamide is heated with sodium hydroxide solution. This reaction prompts the hydrolysis of the amide group and converts it to a carboxyl group, resulting in 2 - (4 - morpholino) ethanesulfonic acid After the reaction is completed, a pure product can be obtained after appropriate separation and purification steps, such as adjusting pH value, crystallization, filtration, drying, etc.
Others use 4-morpholine ethanol and chlorosulfonic acid as raw materials. First, 4-morpholine ethanol is reacted with chlorosulfonic acid, and the sulfonyl chloride group of chlorosulfonic acid replaces the alcohol hydroxyl group to generate 2- (4-morpholine group) ethylsulfonyl chloride. Subsequently, the sulfonyl chloride is treated with alkali solution, and the target product 2- (4-morpholine group) ethanesulfonic acid can be obtained by hydrolysis. Similarly, the subsequent process of separation and purification is required to improve the purity of the product.
These two methods are used in laboratories and industrial production, each with its own advantages and disadvantages, and must be selected according to actual needs and conditions.
