2-Hydroxy-3-[[2-Hydroxy-1,1-Bis(Hydroxymethyl)Ethyl]Amino]Propanesulphonic Acid

2-Hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propane sulfonic acid, its English name is 2-Hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propane sulfonic acid, often referred to as TAPSO. This substance is widely used in biochemistry, molecular biology and other fields.
In biochemical experiments, it is often used as a buffer. Because buffers are essential for maintaining the pH stability of the reaction system, many biochemical reactions are extremely sensitive to pH changes. A slight change in pH may affect the activity of enzymes, the structure and function of proteins, etc. TAPSO has a suitable pKa value, which can effectively resist pH changes within a specific pH range to ensure stable reaction progress. For example, in some enzymatic reactions, precise control of the pH environment is required, and TAPSO can provide ideal working conditions for enzymes with its buffering properties, so that enzymes can fully demonstrate catalytic activity and ensure the smooth progress of the reaction.
In the field of molecular biology, such as the extraction and analysis of nucleic acids. The stability of nucleic acids is easily affected by pH. As a buffer component, TAPSO can maintain the pH stability of the system, prevent the degradation or structural change of nucleic acids due to pH fluctuations during extraction or analysis, and ensure the accuracy and reliability of the experimental results. Moreover, in the separation and purification experiments of proteins, the charge characteristics and solubility of proteins are closely related to pH. TAPSO can adjust and stabilize the environmental pH, helping to achieve efficient separation and purification according to the differences in protein characteristics.

2-Hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propanesulfonic acid, which has specific properties and is worth exploring. Its appearance is mostly white crystalline powder, just like the first snow in winter, pure and delicate.
In terms of solubility, it is easily soluble in water, just like ice and snow integrated into a stream, quietly disappearing, but giving water its unique chemical properties. In organic solvents, its solubility is relatively limited, and it can only be seen in some specific polar organic solvents. It slowly disperses and merges into it.
When it comes to stability, under normal conditions, this substance is like a calm old man, quite stable and able to maintain its own structure. However, once it encounters strong acid and alkali, it will be like a calm lake thrown into boulders, the structure is easily damaged, and the chemical properties will also change. It is also more sensitive to temperature. Under high temperature, it is like a delicate flower, which will gradually lose its original stability and cause decomposition or other chemical changes.
In addition, it has a certain buffering performance, like a mediator, which can effectively maintain the pH stability of the solution within a specific pH range, ensuring that the chemical reaction can proceed smoothly in a suitable acid-base environment. In the field of biochemical research and related fields, this buffering property is like a fixed needle, playing a crucial role in providing a stable chemical environment for many experiments and production processes.

2-Hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propane sulfonic acid, often referred to as TAPSO, is prepared as follows:
First, with suitable starting materials, when taking compounds containing hydroxyl and amino groups, such as 2-amino-2-hydroxymethyl-1,3-propanediol, react with reagents containing sulfonic acid groups.
Wash and dry the reaction vessel first to ensure an anhydrous environment. Take an appropriate amount of 2-amino-2-hydroxymethyl-1,3-propanediol, place it in a reactor, add an appropriate amount of organic solvent, such as dichloromethane or N, N-dimethylformamide, and stir to fully dissolve it.
Then, slowly add a sulfonic acid-containing reagent, such as sodium 3-chloro-2-hydroxypropane sulfonate, with stirring. The dripping speed should be carefully controlled to prevent the reaction from being too violent. After the dripping is completed, heat up to a suitable temperature, such as 40-60 ° C, and continue to stir for a certain period of time, about 6-10 hours. During this process, closely observe the changes in the reaction system.
After the reaction is completed, cool the reaction solution to room temperature. Then, add an appropriate amount of water to the reaction solution to dissolve the unreacted substances and the generated salts. Next, use a suitable acid, such as hydrochloric acid, to adjust the pH value of the reaction solution to the acidic range, so that the product precipitates in the form of free acid.
After that, the product is separated by filtration or extraction. If the extraction method is used, a suitable extractant such as ethyl acetate can be selected, and multiple extractions can be used to improve the yield of the product. Combine the extracts and dry them with a desiccant such as anhydrous sodium sulfate to remove the moisture.
Finally, the organic solvent is removed by reduced pressure distillation to obtain the crude product. The crude product is recrystallized, and a suitable solvent is selected, such as ethanol-water mixed solvent, and multiple recrystallization can obtain 2-hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propane sulfonic acid with higher purity. In this way, the desired product can be obtained.

2-Hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propanesulfonic acid, often referred to as TES, is a biological buffer. Its reaction characteristics with other compounds are quite unique.
Looking at its structure, it contains hydroxyl groups, amino groups and sulfonic acid groups. Hydroxyl groups are hydrophilic and can participate in the formation of hydrogen bonds. In the reaction, they can interact with compounds containing active hydrogen receptors by hydrogen bonds, such as weak interactions with carbonyl compounds, which affect the reaction process.
The amino group is basic and can neutralize with acids. When encountering organic or inorganic acids, the amino group is easy to accept protons and generate corresponding ammonium salts. For example, when reacting with hydrochloric acid, the amino nitrogen atom accepts hydrogen ions to form positively charged ammonium ions, which is crucial in regulating the pH of the reaction system.
Sulfonic acid groups have strong acidity and are easy to ionize hydrogen ions in water, making the compound have good water solubility. And sulfonic acid groups can participate in reactions as nucleophiles, such as nucleophilic substitution reactions, which can attack suitable electrophilic reagents to achieve structural transformation of compounds.
TES, as a buffer, can react with acids or bases added to the system to maintain a relatively stable pH value of the system. When the acidity of the system is enhanced, its amino group binds hydrogen ions; when the basicity is enhanced, the sulfonic acid group ionizes hydrogen ions to neutralize. This property makes it useful for biochemistry and molecular biology experiments, such as protein and nucleic acid research, to provide a stable pH environment for many biochemical reactions, and to ensure the stability of the structure and function of biological macromolecules, which is of great significance for the smooth progress of the reaction.

2-Hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propane sulfonic acid, this is a biochemical reagent, and many matters need to be paid attention to during storage and transportation.
First, storage temperature is critical. This reagent should usually be stored in a cool and dry place. Generally speaking, the temperature should be maintained at 2-8 ° C. If the temperature is too high, it may cause the reagent to deteriorate, its chemical structure or stability will be damaged, which will affect its performance. If the temperature is too low, it may also cause the reagent to freeze, which will also have adverse effects on its quality.
Second, the influence of humidity should not be underestimated. Be sure to avoid moisture in the reagent, because it has a certain water absorption. After moisture, it may cause its concentration to change, and even cause a chemical reaction, resulting in failure. Therefore, it should be stored in a dry environment, and a desiccant can be considered to maintain a dry environment.
Third, the transportation process must ensure stability. Proper protective measures should be taken to prevent severe vibration and collision, so as to avoid damage to the package. If the package is damaged, the reagent will come into contact with air, moisture, etc., which will easily deteriorate and damage.
Fourth, packaging sealing is extremely important. During storage and transportation, the package must be kept well sealed to prevent air, moisture and other impurities from invading, so as to maintain the stable quality of the reagent.
In summary, when storing and transporting 2-hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propane sulfonic acid, temperature, humidity, stable transportation and packaging sealing are all key factors, and must not be negligent to ensure that the quality of the reagent is not affected.