What are the main uses of 2-Hydroxy-3-Morpholinopropanesulfonic Acid?
2-Hydroxy-3-morpholinpropanesulfonic acid, referred to as HEPES, is a crucial reagent in biochemical research. Its main uses are as follows:
First, as a biological buffer. In biochemical and cell biology experiments, the normal function and structure of cells and biomolecules need to be maintained within a specific pH range. HEPES has excellent buffering ability, which can stabilize the pH of the reaction system near a specific value, free from factors such as carbon dioxide and metabolites. For example, during cell culture, cell growth is extremely sensitive to environmental pH. HEPES can maintain the pH of the medium in a suitable range for cell growth, usually 7.2-7.4, so as to ensure normal cell metabolism and proliferation.
Second, it is used for protein and enzyme research. The activity of proteins and enzymes is quite sensitive to the pH of the environment. HEPES can create a stable pH environment, which helps researchers to accurately explore the structure and function of proteins and enzymes under specific conditions. For example, when studying the activity of a protein enzyme, using HEPES buffer to maintain a stable pH can accurately determine the activity of enzymes at different substrate concentrations.
Third, it is also used in the field of in vitro diagnostics. The reaction system of some in vitro diagnostic reagents also needs to stabilize the pH environment to ensure the accuracy and reliability of the detection results. The HEPES buffer system can meet this requirement, such as some immunodiagnostic reagents, which use HEPES to maintain a suitable pH, so that the antigen-antibody reaction can proceed smoothly, and then accurately detect the content of the target substance in the sample.
What are the physical properties of 2-Hydroxy-3-Morpholinopropanesulfonic Acid?
2-Hydroxy-3-morpholine propanesulfonic acid, if its shape is colorless to yellowish transparent viscous liquid, has a slightly bitter taste and is hygroscopic.
Looking at its physical properties, the boiling point is quite high, about 270 ° C to 280 ° C. Due to the strong hydrogen bonding and intermolecular forces within the molecule, it needs high temperature to boil. The melting point is in the range of 27-30 ° C, and it is liquid under normal conditions, but it is easy to solidify at low temperatures.
It has good solubility and can be well dissolved in polar solvents such as water and alcohol. This is due to the fact that the molecule contains polar groups and has strong interactions with polar solvent molecules. The density is about 1.18 - 1.22 g/cm ³, which is slightly larger than water, and will sink when placed in water.
In addition, its flash point is also high, around 148 ° C, indicating that its flammability is relatively low, and the safety of storage and use is guaranteed.
And 2-hydroxy-3-morpholine propanesulfonic acid has good stability and is not easy to decompose and deteriorate under general conditions, which makes it widely used in many fields, such as biological buffer systems, pharmaceutical and chemical industries, etc., providing stable and reliable chemical reagents for related research and production.
What is the difference between 2-Hydroxy-3-Morpholinopropanesulfonic Acid applications in different fields?
2-Hydroxy-3-morpholinpropanesulfonic acid, also known as MOPS, has applications in biochemistry, molecular biology, cell culture and other fields, with significant differences in application in various fields.
In biochemical research, MOPS is often used as a buffer because it can maintain the stability of the pH value of the reaction system and avoid the impact of large fluctuations in pH on the activity and structure of biomolecules. During protein purification, MOPS buffer can maintain a suitable pH environment to prevent protein denaturation and ensure its biological activity. In enzymatic reactions, stabilizing pH can keep the enzyme in the best active state and ensure the efficient progress of the reaction.
In molecular biology experiments, MOPS plays a key role in nucleic acid electrophoresis buffer. During nucleic acid electrophoresis, the appropriate pH environment has a significant impact on the migration rate and separation effect of nucleic acids. MOPS buffer can create a stable pH environment and help the effective separation and analysis of nucleic acids. In addition, in RNA-related experiments, such as Northern hybridization and in vitro transcription, MOPS has a significant effect on RNA stability, can prevent RNA degradation, and ensure the smooth progress of the experiment.
In the field of cell culture, MOPS is an important component of cell culture media. Cell growth is extremely sensitive to environmental pH, MOPS can maintain the pH stability of the medium and provide a suitable microenvironment for cell growth. Especially in adherent cell culture, stabilizing pH is extremely critical for cell adhesion and growth, and MOPS can effectively ensure the normal physiological function and growth and proliferation of cells.
In summary, 2-hydroxy-3-morpholinpropanesulfonic acid has different application methods and focuses in different fields due to differences in the object of action and the purpose of the experiment, but its core function is to maintain the pH stability of the system to ensure the activity of biomolecules, the smooth progress of the experiment, and the normal growth of cells.
What are the storage conditions for 2-Hydroxy-3-Morpholinopropanesulfonic Acid?
2-Hydroxy-3-morpholinpropanesulfonic acid, often referred to as MOPS, is a commonly used buffer in biochemical experiments. Its storage conditions are quite critical, as detailed below:
First, it needs to be placed in a cool place. Due to high temperature, it is easy to cause chemical changes of the substance, which will damage its buffering properties. As "Tiangong Kaiwu" said: "Everything is changeable when exposed to heat." The same is true for this agent. In a cool place, it can reduce the influence of temperature on it and ensure its stability.
Second, it should be stored in a dry place. Because of its hygroscopicity, it is easy to agglomerate or deteriorate in case of moisture. The ancients said: "It can dry solid matter, and if it is wet, it will rot." Keep it dry to avoid its quality degradation due to moisture absorption.
Third, it should be placed in a sealed container. If exposed to air, it is easy to react with the ingredients in the air, or absorb water vapor and carbon dioxide. A sealed device is like a protective barrier, which can block external interference and keep it pure.
Fourth, keep away from fire sources and oxidants. Although this agent is not flammable and explosive, it is still dangerous in case of fire or oxidants. As recorded in ancient books, flammable things can be ignited in case of fire. Although MOPS is not such, it is necessary to be cautious and keep away from fire sources and oxidants to ensure safety. In conclusion, the storage of 2-hydroxy-3-morpholinpropanesulfonic acid should be stored in a cool, dry place, in a sealed container, and away from fire sources and oxidizing agents, so as to maintain its good performance for a long time.
2-Hydroxy-3-Morpholinopropanesulfonic advantages of Acid over other similar compounds
2-Hydroxy-3-Morpholinopropanesulfonic propanesulfonic acid (MOPS) has many advantages over other similar compounds.
MOPS has good buffering performance and can effectively maintain the pH stability of the system in a specific pH range. This is because of its unique molecular structure, containing dissociable groups, in case of acid-base changes, it can be buffered by proton transfer. For example, in biochemical experiments, the pH of the system often fluctuates due to the reaction. MOPS can maintain the pH in an appropriate range to ensure the activity and reaction of biological macromolecules. Compared with other analogs, the buffering effect is better and the stability is better.
Furthermore, MOPS has stable chemical properties and is not easy to side-react with other substances in the system. In complex chemical reactions or biological systems, this is crucial. It can ensure the purity of the system and does not introduce impurities to interfere with the reaction or experimental results. Some similar compounds, or due to their active structure, are easy to react with other substances, which affects the stability and experimental accuracy of the system.
MOPS also has good solubility and can be well miscible with water and various organic solvents. This makes it easy to apply in different experimental conditions and reaction systems. Whether it is an aqueous phase reaction or a system involving some organic solvents, MOPS can quickly dissolve and play a buffering role. Compared with some similar compounds with poor solubility, it has a wider range of applications and is more convenient to use.
In addition, MOPS is less toxic to biological systems. In biomedicine, cell culture and other fields that require high biological activity, the use of MOPS can reduce the adverse effects on organisms or cells and maintain the normal physiological function of biological systems to the greatest extent. However, some similar compounds may have high toxicity, limiting their application in biological-related fields.