2-(Morpholin-4-Yl)Ethanesulfonic Acid

2-%28Morpholin-4-Yl%29Ethanesulfonic+Acid is 2- (morpholine-4-yl) ethanesulfonic acid, often referred to as MES. This is a kind of zwitterionic buffer commonly used in biochemical research. The following will describe its chemical properties in detail:
MES has a white crystalline powder appearance, relatively stable properties, good water solubility, can quickly dissolve in water to form a uniform solution, this characteristic makes it very convenient for the preparation of buffer solutions in biological systems.
The pKa value of MES is about 6.15, and it can show excellent buffering ability in the range of pH 5.5-6.7. This pH range is similar to the suitable pH value of the environment or biochemical reaction in many organisms. Therefore, when simulating physiological conditions or specific biochemical reactions, MES can effectively maintain the pH value of the solution and avoid adverse effects on biomolecules or chemical reaction processes due to pH fluctuations.
In its chemical structure, the sulfonic acid group (-SO 🥰 H) endows the molecule with acidic characteristics, while the morpholine ring provides a certain alkalinity. The synergistic effect of the two creates the zwitterionic characteristics of MES. This structural characteristic not only contributes to its buffering performance, but also affects its interaction with biomolecules. Due to its relatively stable structure, MES generally does not chemically react with common biomolecules, such as proteins, nucleic acids, etc., thus not interfering with normal biochemical analysis and research.
In addition, MES has a weak complexation ability for most metal ions, which makes it impossible to change the reaction balance or affect the catalytic activity of metal ions due to binding with metal ions in biochemical reactions or systems containing metal ions, ensuring the stability and reliability of the experimental system.

2-%28Morpholin-4-Yl%29Ethanesulfonic+Acid is 2- (morpholine-4-yl) ethanesulfonic acid, often abbreviated as MES. Common uses of this substance are as follows:
First, MES is a key buffer in biochemistry and molecular biology experiments. Because it has a specific pH buffer range, about pH 5.5 - 6.7, it is like a stable dam, which can effectively maintain the pH value of the solution. In many enzymatic reactions, the activity of enzymes is extremely sensitive to the environmental pH. MES buffer can create a suitable pH environment to maintain the optimal activity of enzymes, just like providing a comfortable "working room" for enzymes. For some nuclease reactions that depend on specific acidic environments, MES buffer can precisely regulate the pH of the reaction system to ensure the smooth progress of the reaction.
Second, in the field of cell culture, MES is also indispensable. Cell growth requires strict pH of the environment, and MES buffer can maintain the pH of the cell culture medium at an appropriate range, creating a stable living environment for cells, just like building a comfortable "home" for cells. Especially in some adherent cell cultures that are sensitive to pH changes, MES helps cells grow and proliferate normally, avoiding damage to cells due to pH fluctuations.
Third, in protein crystallization experiments, MES buffer has a wide range of uses. The protein crystallization process requires fine environmental conditions, and pH is one of the key factors. MES buffer can provide a stable pH environment for protein crystallization, promote the orderly arrangement of protein molecules, and finally form high-quality crystals, which is like an ideal "framework" for protein crystallization. It helps researchers to analyze protein structures with X-ray crystallography technology and deeply explore the mysteries of protein function.

2-%28Morpholin-4-Yl%29Ethanesulfonic+Acid is 2- (morpholine-4-yl) ethanesulfonic acid, often referred to as MES. When storing, there are many precautions to be paid attention to.
MES is prone to moisture absorption, so the drying of the storage environment is the key. It should be stored in a dry place to avoid moisture caused by air humidity. If it is damp, it will not only affect its chemical purity, but also change its physical properties, which may have adverse effects on subsequent use.
Temperature is also a factor that cannot be ignored when storing MES. It needs to be placed in a cool environment, away from heat sources and direct sunlight. Excessive temperature may trigger its chemical reaction and lead to deterioration. Generally speaking, the recommended storage temperature is between 2-8 ° C. In this temperature range, its stability can be effectively maintained.
MES should be stored in a well-sealed container. Sealing can not only prevent it from reacting with gases such as oxygen and carbon dioxide in the air, but also avoid other impurities from mixing. If using a non-sealed container, impurities in the air may contaminate the MES, thus affecting its quality.
In the area where MES is stored, avoid storing with strong oxidants, strong bases and other substances. MES has a certain chemical activity, and contact with these substances is very likely to cause chemical reactions, resulting in its failure.
When storing MES, be sure to follow relevant regulations, do moisture-proof, temperature control, sealing, etc., and pay attention to isolation from other substances, so as to ensure that its quality and performance are not damaged for subsequent experiments or production.

2 - (morpholine-4-yl) ethanesulfonic acid, often referred to as MES, is a commonly used buffer in biochemical research. The preparation method is as follows:
First take an appropriate amount of morpholine and vinyl sulfonate as starting materials. In a clean and dry reactor, add an organic solvent, such as anhydrous ethanol or dichloromethane, which needs to be refined in advance by dehydration to ensure a suitable reaction environment. Place the reactor in a low-temperature cooling device and slowly cool it down to about 0 ° C - 5 ° C. Under stirring state, slowly add vinyl sulfonate dropwise to the organic solvent containing morpholine according to a certain molar ratio. This process requires strict control of the dripping speed to prevent the reaction from being too violent. After the dropwise addition is completed, the temperature is gradually raised to 30 ° C - 40 ° C, and the reaction is maintained at this temperature for several hours. During this period, the reaction progress is closely monitored and can be tracked by means of thin layer chromatography (TLC).
When the reaction reaches the desired level, the reaction mixture is transferred to a separation funnel and washed several times with an appropriate amount of saturated salt water. The purpose is to remove unreacted raw materials and by-products. The organic phase is separated and dried with anhydrous sodium sulfate to remove residual water. Subsequently, the organic solvent is removed by vacuum distillation through a rotary evaporator to obtain a crude product.
The crude product is separated and purified by column chromatography. A suitable silica gel is selected as the stationary phase, and petroleum ether and ethyl acetate are mixed in a certain proportion as the mobile phase. Collect the fractions containing the target product, and then concentrate it under reduced pressure to obtain pure 2- (morpholine-4-yl) ethanesulfonic acid. If the purity of the product still does not meet the requirements, it can be further purified by recrystallization operation. Select a suitable solvent, such as ethanol-water mixed solvent, heat to dissolve the product, slowly cool it, and allow crystals to precipitate. Filtration and drying are performed to obtain a high-purity target product.

2-%28Morpholin-4-Yl%29Ethanesulfonic + Acid is 2- (4-morpholinyl) ethanesulfonic acid, often abbreviated as MES, is a biological buffer. Its impact on the environment can be viewed from the following aspects.
First of all, its chemical properties, MES has a certain pH buffering ability, and it can maintain the pH stability of the system in a specific pH range. If it enters the natural water body, the pH value of the water body may be affected due to the buffering characteristics. The delicate balance of pH value of the natural water body is of great significance to aquatic organisms. Once it changes due to MES intervention, it may cause physiological disorders of many aquatic organisms. For example, some fish and amphibians reproduce and grow, which have strict requirements on the pH value of the water body, deviate from the appropriate pH range, or cause the reproduction success rate to drop sharply, and the larval development is hindered.
Furthermore, although MES is an organic compound, its structure contains nitrogen, sulfur and other elements. It is degraded and transformed by microorganisms in the environment. If the degradation is incomplete, the accumulation of nitrogen and sulfur intermediates may lead to eutrophication of the water body. Eutrophication causes algae and other plankton to grow wildly, blocking sunlight, affecting the photosynthesis of underwater plants, and then destroying the ecological balance of the water body. Moreover, algae die and decompose after excessive reproduction, consume a lot of dissolved oxygen, cause hypoxia in the water body, and aquatic animals may die due to asphyxiation.
In addition, when MES accumulates in the soil environment, it may affect the soil physicochemical properties. It may change the soil pH and interfere with the availability of nutrients in the soil. Soil microbial communities are crucial to soil ecological functions, and MES exists or affects the types, quantity and activity of microorganisms. Soil microorganisms participate in key processes such as organic matter decomposition and nutrient cycling. Once disturbed by MES, soil fertility and structure may be damaged, affecting plant growth and development.
In summary, although 2- (4-morpholinyl) ethanesulfonic acid is widely used in experiments and other fields, its potential impact on the environment should not be underestimated. It is necessary to properly dispose of wastes containing this substance to prevent them from entering the environment in large quantities to protect the ecological environment.