2-Morpholinoethanesulfonic Acid

2 - Morpholinoethanesulfonic Acid, Chinese name 2 - alkaline ethanesulfonic acid, is often used for MES. Its chemical properties are special.
MES is a white crystalline powder with good solubility in water and can form a homogeneous solution. This is because it contains an aqueous group in the molecule, which can interact strongly with water molecules.
In terms of acidity, MES is a good biological performance. Its pKa value is around 6.15, and it exhibits excellent performance at pH 5.5 - 6.7. The acidity of the environment varies, and MES molecules can release or receive particles, effectively maintaining the pH value of the solution.
In terms of chemical characterization, the MES phase is determined under normal and general environmental conditions. However, if it exists in special conditions with high temperature, oxidation or low temperature, its molecules may be affected and biochemically reversed.
In addition, MES polymorphism has a certain ability to combine, which makes it possible to affect the activity and phase reversal process of polymorphism in some chemical or biological systems involving polymorphism. Due to its specialization, MES has been widely used in multiple domains such as biochemistry, cytoculture, etc., to determine the acid-holding system to ensure the normal biochemical reaction and biological activity of the phase.

2 - Morpholinoethanesulfonic Acid, common to MES, in biological applications, the use of a wide range.
First, MES is often used as a chemical agent. The transformation of biological agents is reversed, and it needs to be able to be realized under a specific pH value. Such as the study of protein, the function of protein is easily affected by the pH value of the environment. MES can be used to maintain the pH value of the system, so that the protein can maintain the inherent activity of the image, and the preservation of the phase can be improved.
Second, in the field of cell culture, MES also plays an important role. The environment of cell production is demanding, and the setting of pH value is of paramount importance. MES can determine the pH value of the cell culture solution, and determine the survival environment of the cell, promote the normal growth and proliferation of the cell, and avoid damage caused by pH wave.
Third, some enzymatic reactions also depend on MES. The catalytic activity of enzymes depends on the pH value of the environment, and the MES system can make enzymes at the lowest pH value, improve the catalytic efficiency of enzymes, and help researchers to further explore the role of enzymes and biochemical reactions.
Therefore, 2-Morpholinoethanesulfonic Acid, with its characteristics of pH determination, plays an indispensable role in many aspects of biology, such as protein research, cell culture, enzymatic reactions, etc., and has a strong impact on biological research.

2 - Morpholinoethanesulfonic Acid, Chinese name 2 - alkaline ethanesulfonic acid, often used as MES. This product is often used as a liquid component in biochemical research. It is very important to store the best parts in order to maintain its efficacy.
The first thing to do is to avoid moisture invasion. In the tidal environment, it is easy to cause its hydrolysis and other processes, so it needs to be placed in the dry place to avoid water vapor invasion. It can be equipped with dry vessels near the dry vessel, such as silicon dry vessel, to absorb the surrounding area.
Secondly, it is appropriate to control the temperature. Generally speaking, normal or slightly lower temperature is better. Usually, it is recommended to refrigerate at 2-8 ° C. This temperature can reduce the rate of degradation and prevent its degradation. If the temperature is high, the molecular activity increases, and it is easy to decompose or react to other substances; if the temperature is low, or there is crystallization, etc., it will also affect the quality of the product.
Furthermore, it needs to be protected from light. Light can make some chemical reactions, so that the MES properties can be changed. Therefore, it should be stored in opaque containers, or hidden in dark, to prevent ultraviolet light and light exposure.
In addition, storage vessels are also studied. It is appropriate to use materials with certain chemical properties, such as glass or specific plastic containers. Because it does not affect the biochemical effect of MES, its temperature can be maintained. Plastic containers need to be resistant to chemical corrosion and avoid being mixed by MES intrusion.
Therefore, in order to properly store 2-Morpholinoethanesulfonic Acid, it must be combined with dryness, radiation, light protection and good equipment, in order to ensure its effectiveness in use as before, and in biochemical research and other fields.

2 - Morpholinoethanesulfonic ethanesulfonic acid (MES) is a commonly used buffer in biochemical research, and it is prone to many common reactions with other substances.
If it encounters a strong base, such as sodium hydroxide (NaOH), the sulfonic acid group of MES will react with it to form the corresponding salt and water. This is an acid-base neutralization reaction. The sulfonic acid group is acidic and can bind to hydroxide ions. The ionic equation is: $R - SO_ {3} H + OH ^ {-}\ longrightarrow R - SO_ {3 }^{-} + H_ {2} O $ ($R $represents the rest of the MES structure).
Encounters active metals such as zinc (Zn), and the acidic hydrogen energy of the MES is replaced to generate hydrogen and corresponding metal salts. The reaction formula is roughly: $2R - SO_ {3} H + Zn\ longrightarrow Zn (R - SO_ {3}) _ {2} + H_ {2}\ uparrow $.
When reacting with certain metal oxides, such as zinc oxide (ZnO), the acidity of MES causes it to interact with metal oxides to form salts and water, as follows: $2R - SO_ {3} H + ZnO\ longrightarrow Zn (R - SO_ {3}) _ {2} + H_ {2} O $.
If it encounters a strong oxidant, such as potassium permanganate ($KMnO_ {4} $), some chemical bonds in the MES molecule may be oxidized and broken, resulting in structural changes, and the products are quite complex and vary depending on the reaction conditions.
In organic synthesis scenarios, if a suitable activator is available, the sulfonic acid group of MES may participate in esterification, substitution, and other reactions, and react with alcohols to generate sulfonates. The specific reaction also depends on the reaction conditions and the accuracy of the reagents used.

2-Morpholinoethanesulfonic ethanesulfonic acid (MES) is a commonly used buffer in biochemical research. There are several common methods for preparation.
First, morpholine and 2-chloroethanesulfonic acid are used as raw materials. First, an appropriate amount of morpholine is placed in the reaction vessel, and the solution of 2-chloroethanesulfonic acid is slowly added dropwise. At the same time, the reaction is stirred at a suitable temperature. This process requires strict control of temperature and reaction time. Due to high temperature or too long time, side reactions may occur and the purity of the product will be reduced. After the reaction is completed, the raw MES can be obtained through neutralization, concentration, crystallization and other steps. Complex through purification methods such as recrystallization to improve the purity of the product.
Second, use 2-hydroxyethanesulfonic acid and morpholine as the starting materials. Mix 2-hydroxyethanesulfonic acid and morpholine in a certain proportion, add an appropriate amount of catalyst, and catalyze the reaction under a specific temperature and pressure. After the reaction is completed, remove the catalyst, separate and purify it, such as ion exchange resin method or organic solvent extraction method, etc., to obtain pure MES.
Third, use morpholine and ethylene sulfonate lactone as raw materials. The reaction of morpholine and ethylene sulfonate lactone in a suitable solvent is relatively mild, but the purity of the raw material and the reaction conditions are quite high. After the reaction, the product is separated and purified by distillation, crystallization and other methods.
When preparing MES, the preparation method should be carefully selected according to the actual needs and conditions. And each step requires fine operation and strict control of the reaction conditions, so that high-purity 2-morpholine ethanesulfonic acid can be obtained, which is suitable for various biochemical experiments and studies.