2-Morpholinoethanesulfonic Acid, Monohydrate

2 - Morpholinoethanesulfonic ethanesulfonic acid monohydrate (2 - Morpholinoethanesulfonic Acid, Monohydrate), often referred to as "MES monohydrate", has a wide range of uses in biochemical and molecular biology experiments.
First, in the field of cell culture, this substance is a key. When cells are cultured in vitro, they are extremely sensitive to the pH of the environment in which they are located. MES monohydrate can stabilize the pH value of cell culture medium within a specific range, usually between pH 5.5 and 6.7, due to its excellent buffering ability. Most cells can grow and metabolize normally within this pH range, and various enzymatic reactions within them can also proceed smoothly. If the pH value fluctuates too much, the physiological function of the cell may be affected, or even apoptosis.
Second, MES monohydrate is also indispensable in the study of enzyme activity. Many enzymes can exhibit optimal activity only under suitable pH conditions. By formulating a buffer system containing MES monohydrate, researchers can simulate the acid-base environment of the enzyme in the organism, and accurately explore the catalytic characteristics and kinetic parameters of the enzyme. Different enzymes require different optimal pH, and the stable pH environment constructed by MES monohydrate provides convenience for the study of enzyme activity.
Third, in the protein crystallization experiment, MES monohydrate has a significant effect. Protein crystallization requires strict environmental conditions, and pH is one of the important factors. MES monohydrate can adjust the pH of the crystallization solution, promoting the orderly arrangement of protein molecules and then crystallization. Obtaining high-quality protein crystals is of great significance for analyzing the three-dimensional structure of proteins through X-ray crystallography, which helps scientists to deeply understand the function and mechanism of protein action.

The purity standards of 2 - Morpholinoethanesulfonic Acid, Monohydrate (2 - morpholine ethanesulfonic acid monohydrate, referred to as MES monohydrate) are not contained in Tiangong Kaiwu. However, in today's chemical industry, the purity standards often vary according to the use.
In general biochemical experiments, such as buffer preparation, the purity of this compound often needs to reach more than 99.0%. The Gain experiment requires strict accuracy and stability of the buffer system, and insufficient purity can easily lead to deviation of buffer capacity, which affects the accuracy of the experimental results. High purity can ensure that it maintains stable buffering performance in a specific pH range, ensuring that biochemical reactions are carried out in a suitable acid-base environment.
And used in high-end pharmaceutical research and development, analytical chemistry and other more precise fields, its purity requirements are higher, or up to 99.5% or even more than 99.9%. Pharmaceutical research and development is related to human health, and very small impurities may also affect the safety and effectiveness of drugs; high-precision analytical testing in analytical chemistry needs to avoid impurities interfering with the analysis results.
Therefore, the purity standard of 2 - Morpholinoethanesulfonic Acid, Monohydrate does not have a fixed single value, and it depends on the specific application scenarios and requirements. It varies from more than 99.0% in general experiments to nearly 100% in high-end fields.

2 - Morpholinoethanesulfonic ethanesulfonic acid monohydrate (2 - Morpholinoethanesulfonic Acid, Monohydrate) is a commonly used reagent for biochemical experiments. When storing, many matters need to be paid attention to.
First environmental conditions. This reagent should be stored in a cool and dry place. If the ambient temperature is too high, it is easy to cause the reagent to deteriorate, and the performance will be affected due to high temperature or cause changes in its chemical structure. Such as in hot summer, if the temperature is too high in the warehouse, the reagent may lose its original efficacy. Humid environment is also not advisable, because it has a certain degree of hygroscopicity, after moisture or agglomeration, and even chemical reactions, it should be ensured that the humidity of the storage place is suitable, and the desiccant can be used to maintain the environment dry.
Second, the package is If the package is damaged, the outside air, moisture, etc. are easily invaded and interact with the reagent. If the package is accidentally damaged after long-term exposure to air, the reagent may react with oxygen to cause oxidation, changing the chemical properties, and then affecting the accuracy of the experimental results.
Furthermore, avoid mixing with other chemicals. Because of its unique chemical properties, coexist with certain chemicals, or have unpredictable reactions. For example, mix with strong oxidizing agents or reducing agents, or cause violent chemical reactions, causing the failure of the reagent, and even pose a safety hazard.
In addition, it is necessary to make labels and records. Mark clearly the key information such as the name of the reagent, specifications, purchase date and valid period. Perfect records are convenient for tracking the use of reagents, and can be processed in time when the valid period is approaching to prevent experimental errors caused by expired reagents.
In short, proper storage of 2-morpholine ethanesulfonic acid monohydrate is essential to ensure its quality and performance, as well as the smooth progress of the experiment and the accuracy and reliability of the results.

2-Morpholine ethanesulfonic acid monohydrate, which is a commonly used reagent for biochemical research. Its physical properties are as follows:
Viewed, it is in the state of white crystalline powder, with pure properties, no variegation and foreign matter, and is easy to identify in experimental observation.
When it comes to solubility, it is easily soluble in water, which makes it rapidly and uniformly dispersed in aqueous solution related experiments, and builds a good environment for subsequent reactions.
In terms of melting point, it is about 125-128 ° C. The melting point is stable, and the phase state transition occurs at a specific temperature, providing a definite reference for the control of experimental conditions.
When it comes to pH, the pH value of its aqueous solution is about 4.0-6.0. This moderate acidity range is suitable for many biochemical reactions with specific requirements for pH, and can effectively maintain the stability of pH in the reaction system.
Its density is about 1.18 g/cm ³, and the density is constant. In operations such as quantitative extraction and mixing, the dosage can be accurately calculated according to this to ensure the accuracy of the experiment.
The physical properties of 2-morpholine ethanesulfonic acid monohydrate lay the foundation for the development of biochemical experiments, helping researchers to accurately design and implement experiments and obtain reliable results.

The chemical synthesis of 2 - Morpholinoethanesulfonic ethanesulfonic acid monohydrate (2 - Morpholinoethanesulfonic Acid, Monohydrate) has various ways. Described in detail below.
First, morpholine and ethylene sulfonic acid or its salts are used as starting materials. Place an appropriate amount of morpholine in a reactor and slowly add the solution of ethylene sulfonic acid or its corresponding salts dropwise. At the same time, strictly control the reaction temperature in a moderate range, generally between 20 and 40 degrees Celsius. This is because the temperature is too high, or side reactions are clustered, which affects the purity of the product; if the temperature is too low, the reaction rate is slow and takes too long. After the dropwise addition is completed, continue to stir the reaction for about 3-5 hours to make the reaction fully proceed. After the reaction is completed, the excess solvent is removed by reduced pressure distillation, and then the recrystallization operation is carried out. The commonly used solvent can be the ethanol-water mixture, so as to improve the purity of the product.
Second, 2-chloroethanesulfonic acid and morpholine can also be used as raw materials. Dissolve 2-chloroethanesulfonic acid in a suitable organic solvent, such as acetone or acetonitrile, and then add an appropriate amount of morpholine. In this process, add an appropriate amount of acid binding agent, such as potassium carbonate or triethylamine, to absorb the hydrogen chloride generated by the reaction and promote the positive reaction. The reaction temperature should be controlled at 50-70 degrees Celsius, and the reaction time is about 4-6 hours. After the reaction is completed, the generated salts are filtered off, and the filtrate is concentrated and separated by column chromatography. Pure 2-morpholine ethanesulfonic acid can be obtained, and then hydrated to obtain 2-morpholine ethanesulfonic acid monohydrate.
There is also a method of using morpholine and sulfonactone as raw materials. Take an appropriate amount of sulfonactone, and under mild conditions, undergo a ring-opening reaction with morpholine. During the reaction, an inert solvent such as toluene or dichloromethane can be selected, and the reaction temperature is controlled at 0-20 degrees Celsius. After the reaction is completed, the target product 2-morpholine ethanesulfonic acid monohydrate is obtained by conventional separation and purification methods, such as extraction, distillation, crystallization, etc. These synthesis methods have their own advantages and disadvantages, and they need to be carefully selected according to actual needs and conditions.