2-(N-Cyclohexylamino)Ethanesulphonic Acid

2-% 28N - Cyclohexylamino%29Ethanesulphonic Acid is 2- (N -cyclohexylamino) ethanesulfonic acid, often referred to as CHES. The physical and chemical properties of this substance are as follows:
Its properties are mostly white crystalline powders, showing weak acidity, which can partially ionize hydrogen ions in aqueous solution. In terms of solubility, it is easily soluble in water, which makes it easy to dissolve and prepare a solution of the required concentration in chemical reactions and biological experiments of aqueous solution systems. The melting point of
CHES is quite important, about 288-290 ° C. This melting point characteristic helps to purify and identify it under specific temperature conditions. Its pKa value is about 9.3 (25 ° C), which indicates that it has the best buffering ability near pH about 9.3. When the pH of the solution is close to its pKa value, CHES can effectively resist the influence of adding a small amount of acid or base on the pH value of the solution and maintain the relative stability of the pH of the solution. Because of this, it is often used as a buffer in biochemistry and molecular biology experiments, such as in the research experiments of some proteins and enzymes, because it can create and maintain a specific pH environment, ensuring the stability of the structure and activity of biological macromolecules such as proteins and enzymes, so it plays a key role. In addition, CHES chemical properties are relatively stable, and it is not easy to react violently with common chemicals at room temperature and pressure, making it easy to store and use. However, it is still necessary to pay attention to moisture-proof and high-temperature conditions during storage to ensure its quality.

2 - (N-cyclohexylamino) ethanesulfonic acid, often abbreviated as "CHES". This substance is widely used in the fields of biochemistry and molecular biology.
CHES has many uses in the study of proteins and nucleic acids. Because of its good buffering properties, it can maintain the stability of the pH of the solution in a specific pH range, so it is an ideal buffer for protein crystallization, purification and activity research. During protein crystallization, a suitable pH environment is the key, and CHES can create a stable microenvironment, which can help protein molecules arrange in an orderly manner and promote crystallization. In the process of protein purification, the charge state of proteins at a specific pH is different, and the stable pH maintained by CHES can be effectively separated by ion exchange chromatography and other techniques.
In nucleic acid research, CHES is also indispensable. For DNA and RNA extraction, amplification and sequencing experiments, a suitable pH environment is required to ensure the stability of nucleic acid structure and the normal activity of related enzymes. The CHES buffer system can precisely regulate the pH of the reaction system to ensure the smooth progress of nucleic acid experiments. For example, polymerase chain reaction (PCR), which has strict pH requirements on the reaction system, CHES can enable the reaction to efficiently amplify nucleic acid fragments at a suitable pH.
In the field of cell culture, CHES also contributes. Cell growth is sensitive to environmental pH. CHES can be used as a buffer component of cell culture medium to maintain the pH stability of the culture medium, create a good growth microenvironment for cells, promote normal cell growth and proliferation, and prevent cell damage or death due to pH fluctuations.
In addition, CHES is used in drug development-related experiments, such as drug solubility testing, drug-biomacromolecule interaction research, etc., and its buffering properties also provide stable pH conditions for experiments and assist in the drug development process.

2 - (N - cyclohexylamino) ethanesulfonic acid, often abbreviated as CHES, is prepared as follows:
Using 2 - chloroethanesulfonic acid and cyclohexylamine as raw materials
1. ** Preparation of raw materials **:
- Prepare chemically pure 2 - chloroethanesulfonic acid and cyclohexylamine, and at the same time prepare an appropriate amount of organic solvents, such as ethanol, and sodium hydroxide or hydrochloric acid solutions for adjusting the pH of the reaction system.
2. ** Reaction process **:
- Dissolve 2 - chloroethanesulfonic acid in ethanol in a certain proportion, and slowly add cyclohexylamine dropwise under stirring conditions. This process requires strict control of the reaction temperature, generally maintained at 40-60 ° C. Because the temperature is too low, the reaction rate is slow and time-consuming; if the temperature is too high, it may initiate side reactions and affect the purity of the product.
- With the dropwise addition of cyclohexylamine, the chlorine atom in 2-chloroethanesulfonic acid undergoes a nucleophilic substitution reaction with the amino group in cyclohexylamine. The chemical reaction equation is:\ (C_ {2} H_ {5} ClO_ {3} S + C_ {6} H_ {11} NH_ {2}\ rightarrow C_ {8} H_ {17} NO_ {3} S + HCl\).
- Hydrogen chloride gas will be generated during the reaction. By installing a reflux condensation device and connecting a tail gas absorption device, hydrogen chloride can be absorbed with a sodium hydroxide solution to avoid its escape from polluting the environment.
3. ** Product Separation and Purification **:
- After the reaction is completed, the reaction mixture is cooled to room temperature, and then the ethanol is removed by distillation under reduced pressure. At this time, the remaining solid matter in the system is the crude product.
- For further purification of the product, the crude product can be dissolved in an appropriate amount of water, and then the dilute sodium hydroxide solution is used to adjust the pH of the solution to a weakly alkaline state, so that the product exists in the solution in the form of sodium salt.
- Next, the impurity ions are removed through the ion exchange resin column. Finally, the effluent is concentrated and crystallized to obtain high-purity 2- (N-cyclohexylamino) ethanesulfonic acid crystals.
Using ethanolamine and cyclohexylamine as raw materials
1. ** Preparation of raw materials **:
- Prepare chemically pure ethanolamine, cyclohexylamine and an appropriate amount of sulfonating agent, such as chlorosulfonic acid. At the same time, inert organic solvents for dilution and reaction, such as dichloromethane, and sodium hydroxide solutions for neutralizing the reaction products, need to be prepared.
2. ** Reaction process **:
- First dissolve ethanolamine in dichloromethane, slowly add chlorosulfonic acid dropwise under low temperature (0 - 5 ° C) and stirring conditions. This step requires extra care because chlorosulfonic acid is highly corrosive and irritating, and the reaction is exothermic. If the dropwise rate is too fast, the reaction may be out of control.
- Ethanolamine sulfonates with chlorosulfonic acid to form 2-hydroxy ethanesulfonic acid intermediates. The chemical reaction equation is:\ (H_ {2} NCH_ {2} CH_ {2} OH + ClSO_ {3} H\ rightarrow H_ {2} NCH_ {2} CH_ {2} SO_ {3} H + HCl\).
-After the sulfonation reaction is completed, cyclohexylamine is added to the reaction system, heated to 60-80 ° C, and the reaction is continued for several hours. During this process, the hydroxyl group in the intermediate of 2-hydroxy ethanesulfonic acid undergoes dehydration and condensation reaction with the amino group in cyclohexylamine to form 2 - (N-cyclohexylamino) ethanesulfonic acid. The chemical reaction equation is:\ (H_ {2} NCH_ {2} CH_ {2} SO_ {3} H + C_ {6} H_ {11} NH_ {2}\ rightarrow C_ {8} H_ {17} NO_ {3} S + H_ {2} O\).
3. ** Product Separation and Purification **:
- After the reaction is completed, the reaction mixture is cooled to room temperature, and then washed with an appropriate amount of water and saturated salt water to remove unreacted raw materials and by-products.
- The organic phase is separated, dried with anhydrous sodium sulfate, and then distilled under reduced pressure to remove dichloromethane. The obtained crude product is purified by recrystallization. A suitable mixed solvent, such as ethanol-water mixed solvent, can be selected to improve the purity and yield of the product.

2-% (N-cyclohexylamino) ethanesulfonic acid, often referred to as CHES, has several advantages over other similar compounds.
Bearing the brunt, CHES has a unique buffer range. It can exhibit excellent buffering performance between pH 9.3 and 10.7, and this range is just suitable for many specific biochemical reactions and experimental needs. For example, when studying the activity of some basophilic enzymes, this buffer range can maintain the enzyme in a stable and active state, while other similar compounds may be difficult to meet such experimental requirements due to inconsistent buffer ranges.
Furthermore, CHES is chemically stable. Under common experimental conditions, it is not easily deteriorated by factors such as oxidation and hydrolysis. When conducting experiments that require long-term incubation, its stability ensures that the buffer system remains constant throughout the experimental process, making the experimental results more reliable. On the other hand, some similar compounds may have poor stability, which may reduce the buffering capacity or produce side reactions in the middle of the experiment, affecting the accuracy of the experiment.
In addition, CHES has no significant interference with most biomolecules. In experiments involving biological macromolecules such as proteins and nucleic acids, it will not specifically bind to these biomolecules or change their structure and function. This allows CHES to provide a near-natural environment for researchers to obtain more realistic experimental data in experiments aimed at studying the properties of biomolecules. However, some similar compounds may interact with biomolecules, resulting in deviations in experimental results.
Finally, CHES has good solubility. Whether it is in the aqueous phase system or some organic-water mixed systems, it can quickly dissolve and disperse evenly, ensuring the uniformity of the buffer system and facilitating the smooth development of the experiment. Some similar compounds may have poor solubility, limiting their application in some specific experimental systems.

2-% 28N - Cyclohexylamino%29Ethanesulphonic Acid is 2- (N-cyclohexylamino) ethanesulfonic acid, often referred to as CHES. This is a commonly used buffer for biochemical experiments. When storing and transporting, you should pay attention to the following things:
First, the storage environment should be kept dry. Because of its hygroscopicity, if the environment is humid, it is easy to cause moisture, which in turn affects its quality and buffering performance. If placed in a humid place, or cause it to agglomerate, it is difficult to dissolve evenly during use, and the buffering effect is greatly reduced. Therefore, when stored in a dry, well-ventilated place, and sealed, the environment can be maintained dry with the help of a desiccant.
Second, temperature control is critical. Generally speaking, it is recommended to store in a cool place to avoid high temperatures. High temperature may cause changes in its chemical properties, accelerating decomposition or deterioration. Excessive temperature may cause changes in molecular structure and decrease buffering capacity. Under normal circumstances, the storage temperature is preferably 2-8 ° C, and this temperature range can ensure its stability. If it is for transportation, appropriate temperature control measures should also be taken to prevent excessive temperature fluctuations.
Third, avoid contact with oxidants and other substances. 2- (N-cyclohexylamino) ethanesulfonic acid is chemically active. Contact with oxidants or react violently, causing it to fail and even pose a safety hazard. During storage and transportation, be sure to store and transport dangerous chemicals such as oxidants separately to prevent interaction.
Fourth, the packaging must be intact. During transportation, ensure that the packaging is sturdy to prevent the packaging from breaking due to bumps and collisions, and the medicine leaks. Once leaked, not only the medicine is wasted, but also pollutes the environment. If it is not handled properly, it may endanger human health. Therefore, carefully check the packaging before transportation to ensure its tightness and firmness.