What is the chemical structure of 2- (N-Morphinyl) Ethanesulfonic Acid Mes?
2-%28N-Morphinyl%29Ethanesulfonic Acid Mes, which is 2- (N-morpholinyl) ethanesulfonic acid, has the following chemical structure:
This compound contains a morpholine ring, which is a six-membered heterocyclic ring with one oxygen atom and one nitrogen atom in the counterposition. Linked to the nitrogen atom of the morpholine ring is an ethylene group (-CH < CH > CH < -), and the other end of the ethylene group is connected to the sulfonic acid group (-SO < H). Its chemical formula is C H 3NO S, and its relative molecular weight is 195.24.
2- (N-morpholinyl) ethanesulfonic acid is often used as a biological buffer and plays an important role in biochemical experiments. Due to its certain buffering capacity, it can maintain the reaction system in a relatively stable pH range and ensure the normal progress of biochemical reactions. And the chemical properties of this substance are relatively stable, and it can play a good buffering function under different conditions, protecting biological macromolecules such as proteins and enzymes in biological systems from pH fluctuations. This structure endows it with excellent buffering properties, so it is widely used in many biochemical and molecular biology experiments.
What are the main uses of 2- (N-Morphinyl) Ethanesulfonic Acid Mes
2-% (N - Morphinyl) Ethanesulfonic Acid Mes is commonly used in biochemical research. Its main purpose is to use this method, which is described by you.
The first to introduce its research on biomacromolecules. In the study of biological macromolecules such as protein and nucleic acids, it is essential to maintain a specific pH environment. Mes can create a stable weakly acidic pH environment, with a typical pH value between 5.5 and 6.7. In this environment, the activity of biomacromolecules is maintained, just like that of chamber flowers, so that the protein is not affected by the pH, and the helix of nucleic acids can also be maintained intact. This is essential for the study of biomacromolecules.
Furthermore, it is also an important function in terms of cell culture. Enzymes survive in the external environment and are sensitive to pH value. Mes is equipped with a cell culture base, which can make the pH of the culture base set at the level that is suitable for cell growth. In this way, the cells can be replaced and proliferated normally, just like fertile soil for seedlings, and the cells can thrive in the pH environment of the environment, ensuring the benefits of cell culture.
In addition, Mes is also indispensable in the study of enzymatic reactions. Enzymes, the essence of biocatalysis, are closely related to the pH of the environment. The specific pH environment provided by Mes can make enzymes with the best activity, and the enzymatic reaction is efficient. This kind of Mes, like a catalytic booster, enables enzymes to demonstrate their capabilities on the biochemical reaction stage, helping researchers to delve deeper into the mechanism and inverse law of enzymes.
Therefore, 2-% (N-Morphinyl) Ethanesulfonic Acid Mes plays an important role in multi-domain research such as biochemical and cellular biology, and the benefits of scientific research work are promoted.
2- (N-Morphinyl) Ethanesulfonic Acid Mes
For 2-% (N - Morphinyl) Ethanesulfonic Acid Mes, it is commonly used. When it is used, it is very important to pay attention to it.
First of all, the pH of Mes is about 5.5 to 6.7. Therefore, when it is used, the pH of the Mes system should be at this level, and it is not effective at the same time.
For the second time, its solubility should not be ignored. Usually, it should be soluble in water in an amount of water. If it is dissolved, it can be slightly increased. However, the degree of solubility should not be high to prevent its properties from being easy. And if it is dissolved, it should be mixed, and it should be dissolved uniformly.
In addition, some gold seeds of Mes have the ability to combine. If there are gold seeds in the family, it is necessary to observe their interactions to avoid dry effects.
In addition, the physical properties of this family also need to be paid attention to. It has a certain degree of certainty, but it may also be damaged when exposed to high temperature, high temperature or low light. Therefore, it is appropriate to use it in dry, dry and dark environments to ensure its safety.
The use of gold, the degree of determination is also high. According to the purpose and characteristics of the family, the precise matching degree can make the effect of gold just right, so as not to cause the degree of inappropriateness and deviation.
Therefore, use 2-% (N-Morphinyl) Ethanesulfonic Acid Mes, and pay attention to pH, solubility, gold reaction, survival, and temperature. Only in this way can we make the benefits and results credible.
What are the physical properties of 2- (N-Morphinyl) Ethanesulfonic Acid Mes
2-% (N - Morphinyl) Ethanesulfonic Acid Mes is an organic compound. Its physical properties are quite important and relevant to its many applications.
Looking at its morphology, Mes often takes the shape of a white crystalline powder at room temperature and pressure. This morphology is conducive to storage and transportation, and it is convenient to use in many experimental operations. Its powder is fine in texture, easy to weigh accurately, and lays the foundation for the accuracy of the experiment.
When it comes to solubility, Mes is soluble in water, which makes it widely used in experiments and industrial production of aqueous solution systems. In water, Mes can be well dispersed and ionized, showing its chemical activity. Its solubility in water varies with temperature. Generally speaking, when the temperature increases, the solubility also increases to a certain extent. However, in organic solvents, its solubility is relatively limited, which limits its application in some non-aqueous systems. The melting point of
Mes is also a key physical property. It has a specific melting point, and the value of this melting point is an important basis for identification and purity judgment. When Mes is heated to the melting point, it changes from a solid state to a liquid state. This process is relatively clear, and accurate measurement of the melting point helps to determine the purity of the compound. If it contains impurities, the melting point tends to decrease and the melting range becomes wider.
In addition, the density of Mes also has its significance. Although it is not an extremely special density value, it is indispensable to consider the density in some situations involving accurate proportions and volumetric calculations. Knowing its density can accurately control the dosage of each component when preparing a solution or mixed system to ensure the smooth progress of the experiment or production process.
The physical properties of this compound, such as morphology, solubility, melting point and density, are interrelated and jointly determine its applicability and application in different fields.
What is the preparation method of 2- (N-Morphinyl) Ethanesulfonic Acid Mes
To prepare 2 - (N - morpholinyl) ethanesulfonic acid (MES), the method is as follows:
First take an appropriate amount of morpholine and put it in a clean reactor. In addition, take a certain amount of vinyl sulfonic acid in an appropriate container and slowly drop it into the reactor containing morpholine. When adding dropwise, the temperature should be carefully controlled to keep the temperature in the kettle within a specific range, generally 30 to 40 degrees Celsius. This is because the reaction is an exothermic reaction. If the temperature is out of control, it may cause the reaction to be too violent and affect the purity and yield of the product.
Add dropwise and continue to stir to promote a full reaction between the two. The stirring time is about 2 to 3 hours (hours) to achieve a relatively complete reaction.
After the reaction is completed, move the reaction mixture liquid to the distillation device. First, distillate under normal pressure to remove impurities with low boiling point. Then change to vacuum distillation to carefully collect the fraction with a specific boiling point range. This fraction is crude 2 - (N - morpholinyl) ethanesulfonic acid.
However, the crude product still contains impurities and needs to be further purified. The method of recrystallization can be used to select a suitable solvent, such as ethanol-water mixed solvent. Dissolve the crude product in this mixed solvent, heat it until the crude product is completely dissolved, then let it stand, slowly cool down, and allow the crystals to precipitate. When the crystals are fully analyzed, filter with a Brinell funnel, collect the crystals, and then wash with a small amount of cold solvent to remove the impurities attached to the crystal surface. Finally, the crystals are placed in an oven and dried at a suitable temperature to obtain a pure 2 - (N - morpholinyl) ethanesulfonic acid product. The whole preparation process requires strict adherence to operating specifications and attention to details to obtain the ideal product.