2- (N-Morpholine) Ethanesulfonic Acid Monohydrate Mes Monohydrate

2 - (N-morpholine) ethanesulfonic acid monohydrate (Mes Monohydrate) has important uses in many fields such as biochemistry and molecular biology.
In the field of cell culture, it can create and maintain a stable pH environment suitable for cell growth. Cell growth is extremely sensitive to environmental pH. With good buffering ability, Mes Monohydrate can resist pH fluctuations caused by external factors, and keep cells growing in stable acid-base conditions, which is conducive to normal cell metabolism and proliferation.
In the study of enzymatic reactions, it is also indispensable. The activity of many enzymes is closely related to the pH of the reaction environment. Mes Monohydrate can provide a specific pH buffer system according to the needs of the enzyme, so that the enzyme can exhibit activity under the optimal pH conditions, and help researchers accurately explore the mechanism of enzyme action and kinetic parameters.
In the field of nucleic acid research, such as the extraction and analysis of DNA and RNA, it is essential to maintain an appropriate pH. Mes Monohydrate can prevent the structure and function of nucleic acids from being damaged due to improper pH during operation, ensuring the smooth progress of nucleic acid research.
Protein research is also commonly used. The stability, activity and interaction of proteins are all affected by pH. Mes Monohydrate can create a suitable pH environment, keep proteins in their natural conformation and active state, and lay the foundation for protein purification, crystallization, activity analysis and other experiments.
In addition, in the preparation of electrophoresis buffer, Mes Monohydrate participates in the construction of buffer system to ensure pH stability during electrophoresis, so that biological macromolecules can be effectively separated according to their own charge and electric field, and accurate experimental results can be obtained.

2-%28N-Morpholine%29+Ethanesulfonic+Acid+Monohydrate is MES monohydrate, which is a commonly used buffer in biochemical experiments. Its storage conditions are quite important, as follows:
First, in terms of temperature, it should be stored in a low temperature place. Generally speaking, it is better to place it in an environment of 2-8 ° C. Such a low temperature environment can effectively slow down its chemical change rate, avoid decomposition or deterioration due to excessive temperature, and thus maintain its chemical stability and buffering performance for a long time.
Second, humidity cannot be ignored. It needs to be stored in a dry place to avoid moisture. When the humidity is high, MES monohydrate may absorb water, change its purity and properties, and affect the accuracy of the experimental results. Therefore, a sealed container should be selected for storage, or a desiccant should be placed in the storage place to reduce the impact of environmental humidity on it.
Third, light will also have an effect on it. This substance should be stored away from light, because light or luminescent chemical reactions can change the molecular structure and destroy its original properties. It can be stored in a brown bottle, or placed in a dark place where light is difficult to reach.
Fourth, care should be taken to avoid mixing with other chemicals. MES monohydrate has specific chemical properties. If it comes into contact with certain substances or reacts chemically, it should be stored separately from other chemicals to prevent mutual interference.
According to these storage conditions, the quality and performance of the 2-%28N-Morpholine%29+Ethanesulfonic+Acid+Monohydrate can be guaranteed to the greatest extent, and it will play its due role in the experiment.

2-%28N-Morpholine%29Ethanesulfonic Acid Monohydrate, MES monohydrate, is a commonly used buffer in biochemical research. To know its molecular weight, it is necessary to know the atomic weight of each atom.
MES chemical formula is $C_6H_ {13} NO_4S\ cdot H_2O $. The atomic weight of carbon ($C $) is about 12.01, hydrogen ($H $) is about 1.01, nitrogen ($N $) is about 14.01, oxygen ($O $) is about 16.00, and sulfur ($S $) is about 32.07.
In $C_6H_ {13} NO_4S $, the total mass of carbon is 6 dollars\ times12.01 = 72.06 $; the total mass of hydrogen is 13 dollars\ times1.01 = 13.13 $; the mass of nitrogen is 14.01 dollars $; the total mass of oxygen is 4 dollars\ times16.00 = 64.00 $; the mass of sulfur is 32.07 dollars $. The molecular weight of this part is 72.06 dollars + 13.13 + 14.01 + 64.00 + 32.07 = 195.27 $.
For a molecule of crystal water ($H_2O $), the total mass of hydrogen is 2 dollars\ times1.01 = 2.02 $, the mass of oxygen is 16 dollars $, and the molecular weight of crystal water is 2.02 dollars + 16.00 = 18.02 $.
Therefore, the molecular weight of MES monohydrate is 195.27 dollars + 18.02 = 213.29 $.
Therefore, the molecular weight of 2-%28N-Morpholine%29Ethanesulfonic Acid Monohydrate is about 213.29.

2-%28N-Morpholine%29Ethanesulfonic Acid Monohydrate, MES monohydrate, is a commonly used buffer in biochemical research. The pH buffer range of this buffer in the solution is about pH 5.5 to pH 6.7.
MES monohydrate has the buffering ability in this pH range because its chemical structure contains groups that can release and accept protons. When the pH of the solution changes, these groups can interact with protons, thereby inhibiting large changes in the pH of the solution. In this pH buffer range, MES monohydrate can maintain the relative stability of the pH of the solution, creating a suitable acid-base environment for many biochemical reactions and experiments. For example, in some enzymatic reactions that are sensitive to pH value, the enzyme can maintain good activity within this buffer range, so that the reaction can proceed smoothly. For example, in experiments such as cell culture, a suitable pH buffer environment is of great significance to the normal growth and metabolism of cells, and MES monohydrate can play a key role.

2-%28N-Morpholine%29 Ethanesulfonic Acid Monohydrate, MES monohydrate, its purity standards vary in different application scenarios.
In the field of biochemical research, high-purity products are often required, with a purity of up to 99% or more. Such high purity ensures accurate and reliable experimental results, and avoids impurities from interfering with the process and results of biochemical reactions. For example, in enzyme activity determination experiments, impurities may inhibit or activate enzymes, making the experimental data inaccurate. High-purity MES monohydrate can effectively avoid such situations.
In cell culture, the purity requirements are also strict, and the purity is usually about 99%. Cells are extremely sensitive to the culture environment. MES monohydrate acts as a buffer. If the purity is insufficient, impurities may harm cell growth, proliferation, and even cause cell death.
When used as a buffer in general chemical experiments, the purity requirements are slightly lower, and about 98% can also meet the demand. Due to the relatively high tolerance of impurities in general chemical experiments, MES monohydrate can effectively play a buffering role and regulate the pH value of the reaction system at this purity.
In short, the purity standard of 2- (N-Morpholine) Ethanesulfonic Acid Monohydrate depends on the specific application, from about 98% in general chemical experiments to 99% or more in biochemical research, cell culture and other fields, to meet the needs of different scenarios.