2-(N-Morpholino)Ethanesulfonic Acid

2-%28N-Morpholino%29Ethanesulfonic Acid, the Chinese name is 2- (N-morpholino) ethanesulfonic acid. This substance is very important in biochemical research and other fields. It is mostly white crystalline powder and soluble in water. In biological experiments, it is often used as a buffer, which can effectively maintain the stability of the pH value of the system and create a suitable acid-base environment for many biochemical reactions. It can be seen in experimental processes such as cell culture and protein purification. Because of its little interference with biological systems, it is favored by scientific researchers. Under specific reaction conditions, it can interact ingeniously with other substances according to its own structural characteristics to ensure the stable and orderly progress of experiments and help researchers obtain accurate and reliable experimental data.

2-%28N-Morpholino%29Ethanesulfonic Acid is 2- (N-morpholino) ethanesulfonic acid, often abbreviated as MES. This is a commonly used buffer in biochemical and molecular biology experiments. Its main uses are as follows:
First, the field of cell culture. Cell growth is extremely sensitive to environmental pH, MES buffers can effectively maintain the pH stability of the culture medium and create a suitable microenvironment for cell growth. Because of its good buffering ability in a specific pH range, it can ensure normal metabolism and proliferation of cells under stable pH conditions, and avoid damage to cells due to large fluctuations in pH, it is widely used in a variety of animal cells and plant cell culture systems.
Second, protein research. The structure and function of proteins are greatly affected by pH. During the process of protein extraction, purification and activity determination, MES buffer can provide a stable pH environment, prevent proteins from denaturing due to pH changes, and maintain their natural conformation and biological activity. Many protein experiments, such as electrophoresis and chromatographic separation, rely on MES buffers to ensure the smooth progress of the experiment.
Third, the enzymatic reaction system. The catalytic activity of enzymes is highly dependent on specific pH conditions, and MES buffers can provide a suitable pH for enzymatic reactions, so that enzymes can exert the best catalytic efficiency. Different enzymes require different optimum pH. MES buffers can precisely adjust and maintain the pH of the reaction system according to the characteristics of specific enzymes to ensure efficient and stable enzymatic reactions. They are commonly used in the activity detection and kinetic research of various enzymes.
Fourth, the field of nucleic acid research. In the experimental links of nucleic acid extraction, amplification, sequencing, etc., it is indispensable to stabilize the pH environment. MES buffers can prevent the degradation or structural change of nucleic acids due to abnormal pH during operation, ensure the integrity and biological function of nucleic acids, and are of great significance for key experimental steps such as PCR amplification and DNA sequencing. In conclusion, 2- (N-morpholino) ethanesulfonic acid plays a key role in many experimental fields of life science due to its excellent buffering properties, laying a solid foundation for various biomolecular research and cell culture work.

2-%28N-Morpholino%29Ethanesulfonic Acid is 2- (N-morpholino) ethanesulfonic acid, often abbreviated as MES. This is a commonly used zwitterion buffer in biochemical research. Its physical and chemical properties are as follows:
MES has a white crystalline powder appearance and stable properties. It has good solubility in water and can quickly dissolve to form a homogeneous solution, which provides convenience for experimental operation.
It has a specific pH buffer range, about pH 5.5 - 6.7. Within this range, it can effectively maintain the pH value of the solution and resist the pH change caused by the addition of a small amount of acid or base. This property is significant in many biochemical reactions, because the activity and stability of many biomolecules are closely related to environmental pH. For example, in some enzymatic reactions, a suitable and stable pH environment is the key to ensure enzyme activity, and MES buffer can create such conditions.
From the perspective of chemical structure, it contains morpholine ring and ethanesulfonic acid group, which gives it good buffering ability. The presence of morpholine ring helps to regulate the acid-base balance of molecules, while ethanesulfonic acid group enhances its solubility and ionization characteristics in water.
In addition, MES is relatively stable in chemical properties, and it is not easy to decompose or react with common chemicals under normal storage and use conditions, which makes it reliable in different experimental systems, providing stable and effective buffer support for research in biochemistry, molecular biology and other fields.

2-%28N-Morpholino%29Ethanesulfonic Acid is 2- (N-morpholine) ethanesulfonic acid, often abbreviated as MES. This is a commonly used buffer in biochemical and molecular biology experiments. There are many precautions to be taken when storing and transporting.
First words storage. First, it must be placed in a cool and dry place. MES is hygroscopic. If the environment is humid, it is easy to cause its hygroscopic agglomeration, which affects the quality and use effect. Remember the old adage "Hide it in a dry place to prevent moisture change", so the humidity should be controlled at a low level, usually 40% -60% relative humidity. Second, the temperature also needs to be paid attention to. High temperature may cause it to decompose or deteriorate. Generally, it is suitable to store at a temperature of 2-8 ° C. Just as the ancient books say, "Store it in a cool place to avoid its thermal change", and refrigerate it in the refrigerator to ensure its stability. Third, it needs to be stored away from light. Light may cause it to undergo photochemical reactions, which will affect its performance. When stored properly in an opaque container or packaging, it is like the ancients hid things in a dark place to prevent them from being damaged by light.
Let's talk about transportation. First, the packaging must be stable. Due to the inevitable bumps and vibrations during transportation, if the packaging is not good, it is easy to cause damage to the container and MES leakage. When packing with strong packaging materials, such as plastic bottles and buffer materials, just as the ancients transported fragile things, they must be wrapped in thick materials to prevent damage. Second, the temperature conditions also need to be considered. When it is hot in summer, it is advisable to take cooling measures, such as adding ice packs or using refrigerated transportation equipment; when it is cold in winter, it is also necessary to prevent freezing and keep warm properly. Third, avoid mixing with other chemicals. The nature of MES or react with other things. If it is mixed, it may cause accidents. Just like the ancients said "do not mix with different species to prevent it from changing", it should be placed separately during transportation to ensure safety.

2 - (N - morpholine) ethanesulfonic acid, often referred to as MES, is prepared by various methods. The common ones are as follows:
First, morpholine and ethanesulfonic acid or its salts are used as raw materials and prepared by nucleophilic substitution. This reaction needs to be carried out in an appropriate temperature and solvent, and a catalyst is required to accelerate the reaction and improve the yield. The solvent used may be water, alcohol, or polar aprotic solvents such as dimethylformamide (DMF). During the reaction process, the progress of the reaction needs to be closely monitored, and the end point of the reaction needs to be determined by means of thin layer chromatography (TLC) or high performance liquid chromatography (HPLC). After the reaction is completed, the pure MES product can be obtained through separation and purification steps, such as extraction, crystallization, column chromatography, etc.
Second, the morpholine is reacted with 2-chloroethanesulfonic acid. This reaction is also a nucleophilic substitution process, where the chlorine atom is replaced by the nitrogen atom of the morpholine to generate MES. The reaction conditions are quite critical, and the temperature and amount of base will affect the effect of the reaction. Alkali or sodium hydroxide, sodium carbonate, etc. can be used to neutralize the acid generated by the reaction and push the reaction in the positive direction. After the reaction is completed, various separation and purification methods are also required to obtain high-purity products.
Third, with morpholine and ethylene oxide as starting materials, 2-morpholine ethanol is first prepared, and then MES is obtained by sulfonation reaction. The sulfonating agent may be concentrated sulfuric acid, chlorosulfonic acid, etc. The reaction needs to control the temperature and time to prevent side reactions such as excessive sulfonation. The subsequent separation and purification steps are similar to the previous two, aiming to remove impurities and improve product quality.
When preparing 2- (N-morpholine) ethanesulfonic acid, the purity of the raw materials, the precise control of the reaction conditions, and the effect of separation and purification all have a great impact on the quality and yield of the product. It is necessary to carefully optimize the reaction of each step according to the actual situation to achieve the ideal preparation effect.