2-(N-Morpholino)Ethanesulfonic Acid Hemisodium Salt

2-%28N-Morpholino%29Ethanesulfonic Acid Hemisodium Salt is 2- (N-morpholino) ethanesulfonate hemisodium salt, often abbreviated as MES hemisodium salt. This substance has a wide range of uses and is a commonly used buffer in biochemical and molecular biology experiments.
In biochemical experiments, many enzymatic reactions are extremely sensitive to environmental pH. MES hemisodium salt can effectively maintain the pH stability of the reaction system with its specific pH buffer range, so that the enzyme maintains good activity and function. For example, in some protein hydrolysis experiments that depend on enzymes in a specific pH environment, MES hemisodium salt can create suitable reaction conditions to ensure the smooth progress of the hydrolysis reaction.
In the field of nucleic acid research in molecular biology, the extraction, amplification and analysis of nucleic acids require a precise pH environment. MES hemisodium salt can adjust the pH of the reaction solution to ensure the stability of nucleic acid structure and prevent its degradation. Like in polymerase chain reaction (PCR), the appropriate pH has a significant impact on the activity of Taq DNA polymerase. MES hemisodium salt can optimize the reaction conditions and improve the efficiency and accuracy of PCR amplification.
Cell culture is also an important application scenario. Cell growth requires strict pH in the culture environment. Adding MES hemisodium salt to the culture medium can stabilize the pH, create a good growth microenvironment for cells, and facilitate the normal development of physiological activities such as cell adhesion and proliferation. It is of great significance for maintaining the normal shape and function of cells.
In addition, in protein crystallization experiments, suitable pH conditions are one of the key factors for obtaining high-quality protein crystals. MES hemisodium salt can adjust the pH of the crystallization solution, helping researchers obtain ideal protein crystals for subsequent X-ray crystallographic analysis of protein structures.

2 - (N - morpholinic) ethanesulfonic acid semi-sodium salt, its physical and chemical properties are as follows:
This substance is in the state of white crystalline powder, pure in appearance and uniform in texture. In terms of solubility, it is easily soluble in water, just like snowflakes merging into a stream, quietly disappearing in the water, forming a clear solution. This property is convenient for application in experiments or production operations of various aqueous solution systems, and can quickly disperse and participate in reactions.
Its pH is quite critical. As a buffer, it can effectively maintain the pH stability of the solution within a specific pH range. Generally speaking, its applicable pH range is about 6.1-7.5, just like a loyal guard. When external factors try to change the pH of the solution, it will come forward to adjust the concentration of hydrogen ions, so that the pH value is stable as a rock. This can provide a suitable environment for many biochemical reactions or experiments sensitive to pH.
In terms of chemical stability, under conventional storage conditions, it is quite stable and can maintain its own chemical structure and properties for a long time. However, it should be noted that it should be avoided from contact with strong oxidants, strong bases and other substances, otherwise if dry wood encounters fire, it may cause a chemical reaction and cause it to deteriorate and fail.
Its melting point also has a certain range, roughly in a relatively high temperature range. This characteristic indicates that its structure has a certain stability. Under normal temperature fluctuations, it is not easy to change the physical state, thus ensuring that the solid state is maintained during storage and use.
In summary, the physicochemical properties of 2 - (N - morpholino) ethanesulfonic acid disodium salt make it indispensable in many fields such as biochemistry and molecular biology. It is like an exquisite tool to help researchers explore the mysteries of the microscopic world.

2-%28N-Morpholino%29Ethanesulfonic Acid Hemisodium Salt, which is 2- (N-morpholino) ethanesulfonic acid hemisodium salt, is a commonly used buffer for biochemical experiments. When storing, pay attention to the following numbers:
First, the temperature must be controlled. This agent should be stored in a low temperature environment, usually 2-8 ° C. If the temperature is too high, it may cause its chemical properties to change and its stability to be damaged. Because of high temperature, it will promote the molecular movement of its molecules to intensify, or cause decomposition and deterioration reactions, which will affect the accuracy of the experimental results.
Second, humidity should be prevented. It is easy to absorb moisture, so it should be placed in a dry place. Moisture intrusion, or causing deliquescence, not only affects the convenience of access, but also changes its concentration and purity. If the ambient humidity is high, a desiccant can be placed in the storage container to keep it dry.
Third, it is also necessary to avoid light. The substance is sensitive to light or under light, or induces luminescent chemical reactions, causing its structure to change and its activity to decrease. Therefore, brown bottles should be selected for storage or placed in a place protected from light to reduce the impact of light on it.
Fourth, the seal must be tight. The storage container should be well sealed to prevent contact with air. Oxygen, carbon dioxide and other components in the air may react with it. If carbon dioxide can cause its pH to change, oxygen or oxidation is not conducive to its preservation.
Fifth, classified storage. Do not mix with oxidizing agents, reducing agents, acids, alkalis and other chemicals. Due to its chemical properties, coexistence with the above substances, or violent reactions, may cause safety hazards and damage its own quality.
Storage of 2- (N-morpholino) ethanesulfonate semi-sodium salt requires attention to temperature, humidity, light, sealing and classification, so as to ensure its stability and obtain accurate and reliable results for experiments.

If you want to perform the quality test of 2 - (N - Morpholino) Ethanesulfonic Acid Hemisodium Salt, you can follow the following steps.
First sample. When taking this sample, make sure that the one taken is representative. If the sample is a large amount of solid, it should be sampled at multiple points in different parts, and then mixed; if it is liquid, stir well and then take an appropriate amount, so that the sample can truthfully reflect the overall characteristics.
Next, purity detection is indispensable. High-performance liquid chromatography (HPLC) can be used. The sample is properly prepared into a solution and injected into the HPLC system, and the purity is determined according to its peak condition. The standard product is used as a control. If the ratio of the peak area of the sample to the peak area of the standard product is within a specific range, the purity can be proved to meet the requirements.
Moisture content also needs to be determined. Carl Fischer method is commonly used. The Carl Fischer reagent reacts with the water in the sample, and the moisture content is calculated according to the dosage of the reagent. During operation, factors such as environmental humidity should be paid attention to to to prevent interference.
Impurity inspection is also key. In addition to HPLC detectable impurities, mass spectrometry (MS) can also be used. MS can accurately measure molecular mass and help identify impurity types. For possible metal impurities, atomic absorption spectrometry (AAS) or inductively coupled plasma mass spectrometry (ICP-MS) can be used to measure their content.
During the testing process, the instruments used need to be accurately calibrated, and the operator should follow the specifications to ensure that the data is accurate and reliable, so that the true quality of the sample can be obtained.

2-%28N-Morpholino%29Ethanesulfonic Acid Hemisodium Salt is 2- (N-morpholino) ethanesulfonate hemisodium salt, which has the following safety risks during use.
If this product is accidentally exposed to the eyes, it will cause irritation to the eyes, causing eye discomfort, pain, and in severe cases, it may damage vision. If it comes into contact with the skin, it may cause skin irritation, and some people may have allergic reactions, such as rash, itching, redness and swelling. If the dust is inhaled, it will irritate the respiratory tract, causing cough, asthma, breathing difficulties, and long-term or high-concentration inhalation, which will damage the health of the respiratory system. Accidental ingestion will irritate the mouth, throat and stomach, causing nausea, vomiting, abdominal pain, and diarrhea.
Be sure to take good protection when using. Wear appropriate protective glasses during operation to avoid contact with eyes; wear protective clothing and gloves to prevent skin contact; ensure that the operating environment is well ventilated, and it is best to operate in a fume hood to reduce the risk of dust inhalation. If inadvertently exposed, eye contact should be rinsed with a lot of water immediately, and if necessary, seek medical attention; skin contact should be rinsed with soap and water as soon as possible; quickly transfer to a fresh air after inhalation, breathing difficulties need to be oxygenated and seek medical attention; If you eat it by mistake, you should seek medical attention immediately, do not induce vomiting by yourself, and follow the doctor's instructions.