2-(N-Morpholino)Ethanesulfonic Acid Monohydrate

2-%28N-Morpholino%29Ethanesulfonic Acid Monohydrate is 2- (N-morpholino) ethanesulfonic acid monohydrate, which is not recorded in the classic "Tiangong Kaiwu", but according to today's chemical knowledge, its properties are clear.
This compound has specific chemical properties. The appearance is often white crystalline powder, with good solubility, soluble in water and some organic solvents. In aqueous solution, it can exhibit buffering properties and can effectively maintain the pH value of the solution in a specific range. This buffering property makes it widely used in biochemical research, cell culture and other fields, which can maintain the stability of pH in the system and protect the structure and function of biological macromolecules.
In its chemical structure, the morpholine ring is connected to the ethanesulfonic acid group, giving the compound unique reactivity. Because of the acidic sulfonic acid group, it can neutralize with the base to form the corresponding salt. And the compound has good thermal stability. Under normal experimental conditions, it can maintain its own structure and properties stable, which is conducive to various operations and applications.
In short, 2- (N-morpholine) ethanesulfonic acid monohydrate is an indispensable chemical reagent in modern chemistry, biology research and related industrial practices due to its buffering, dissolution and thermal stability properties.

2-%28N-Morpholino%29Ethanesulfonic Acid Monohydrate is 2- (N-morpholino) ethanesulfonic acid monohydrate, often referred to as MES monohydrate. In biological experiments, its common uses are as follows:
MES monohydrate is a biological buffer and is widely used in biochemical and molecular biology experiments. Because it has a suitable pKa value of about 6.15, it has good buffering ability in the range of pH 5.5-6.7, which can effectively maintain the pH stability of the reaction system and avoid pH fluctuations affecting the activity and reaction process of biomolecules. For example, in enzymatic reactions, the activity of enzymes is extremely sensitive to pH, and the MES monohydrate buffer system can ensure that the enzyme is in an optimal pH environment, so that the enzyme activity can be fully exerted and the reaction can be carried out smoothly.
Cell culture also uses MES monohydrate. Cells have strict requirements on the pH of the culture environment. MES monohydrate can adjust and stabilize the pH of the medium to create a suitable environment for cell growth. Especially for some cell lines with poor tolerance to pH changes, the use of MES monohydrate is particularly critical, which can help cells grow and proliferate normally, maintain cell physiological function and morphological integrity.
In nucleic acid-related experiments, such as DNA extraction and PCR amplification, it is also necessary to stabilize the pH environment. MES monohydrate can prevent nucleic acids from being degraded or denatured due to pH changes during operation, ensure the integrity of nucleic acid structure and function, and make subsequent analysis and experimental results accurate and reliable.
In the field of protein research, MES monohydrate has applications in protein isolation, purification and identification. In protein electrophoresis, chromatographic separation and other experimental links, by maintaining a stable pH, to ensure the stability of protein charge state and structure, to avoid protein aggregation or inactivation, to provide reliable conditions for protein research.

2-%28N-Morpholino%29Ethanesulfonic Acid Monohydrate, which is 2- (N-morpholino) ethanesulfonic acid monohydrate, is often referred to as MES monohydrate. The storage conditions of this substance are quite important, which is related to its quality and utility.
MES monohydrate should be stored in a dry and cool place. It should be stored in a dry and cool place due to moisture, which can easily cause deliquescence and damage its purity and characteristics. If placed in a place with high humidity, water molecules may interact with MES monohydrate, changing its chemical structure and properties, affecting subsequent use. And a cool environment can reduce its chemical reaction rate and maintain its chemical stability. High temperature can easily promote its decomposition or initiate other chemical reactions, so it should be avoided.
Furthermore, it must be stored in a dark place. Light or photochemical reactions can change the structure of MES monohydrate and reduce its activity. This drawback can be effectively prevented by storing it in an opaque container or in a dark place.
In addition, the storage place should be away from chemicals such as strong oxidants and strong bases. MES monohydrate has specific chemical properties. Contact with strong oxidants or initiate oxidation reactions. In case of strong bases, reactions such as acid-base neutralization may occur, which will destroy its original structure and function.
When storing 2- (N-morpholino) ethanesulfonic acid monohydrate, keep it dry, cool, shaded, and avoid contact with specific chemicals to ensure its long-term maintenance of good quality and performance for various experiments and applications.

2-%28N-Morpholino%29Ethanesulfonic Acid Monohydrate, which is 2- (N-morpholino) ethanesulfonic acid monohydrate, is often referred to as MES monohydrate. Its purity has a great impact on the experiment and cannot be ignored.
If the purity is insufficient, impurities exist in it. In terms of chemical reactions, impurities may participate in the reaction, resulting in impure products, which makes the reaction results deviate from expectations, just like mixing inferior materials, causing the delicate utensils to be imperfect. In biological experiments, impurities or interfere with the activity of biomolecules, distorting the experimental data, just like foreign objects disrupt the delicate order of living things.
High-purity MES monohydrate can ensure the repetition and reliability of experiments. In the same experiment, different batches of high-purity reagents are used, and the results are similar. For example, precision instruments operate accurately every time. On the contrary, the purity is different, the results fluctuate greatly, and the credibility of the experimental conclusions decreases. It is like a pavilion with an unstable foundation and is difficult to trust.
In pH-sensitive experiments, MES monohydrate is used as a buffer, and the purity affects the buffering capacity. High purity can accurately maintain the stability of the pH value of the system, like a loyal guard guarding the established range. Poor purity, pH fluctuations affect the structure and function of biological macromolecules, and many experiments that rely on stable pH are impacted.
In addition, purity affects the cost and efficiency of experiments. Low purity, or multiple repeated experiments are required to obtain reliable results, which is time-consuming, laborious, and wasteful of resources. It is like a detour and delays the journey. Therefore, in order to achieve the ideal experimental effect and ensure the accuracy, reliability and efficiency of the results, the selection of high-purity 2- (N-morpholine) ethanesulfonic acid monohydrate is a key move.

To prepare a suitable solution of 2 - (N -morpholino) ethanesulfonic acid monohydrate (MES), follow the steps below.
First take an appropriate amount of MES reagent. If you want to prepare a specific concentration solution, the required reagent quality should be accurately calculated according to its molar quality. If you want to make a solution of a certain volume and a specific molar concentration, you can use the formula\ (n = C\ times V\) (\ (n\) is the amount of the substance,\ (C\) is the molar concentration,\ (V\) is the volume of the solution) to calculate the amount of the required MES substance, and then calculate the mass from\ (m = n\ times M\) (\ (m\) is the mass,\ (M\) is the molar mass).
Take a clean volumetric flask and rinse it with deionized water several times. Place the calculated mass of MES reagent in a clean beaker, add an appropriate amount of deionized water, and stir it lightly with a glass rod to promote its dissolution. When the reagent is completely dissolved, slowly inject the solution into the volumetric flask along the glass rod.
Rinse the beaker and glass rod with a small amount of deionized water several times, and the lotion is also injected into the volumetric flask to ensure that the reagent is intact. Then add deionized water to about 1-2 cm below the scale line of the volumetric flask, and use a rubber head dropper to add water dropwise until the concave liquid level of the solution is tangent to the scale line.
Cover the volume cork tightly, press the cork with one hand, and hold the bottom of the bottle with the other hand. Repeatedly invert and shake well to make the solution uniform. In this way, the desired 2 - (N - morpholino) ethanesulfonic acid monohydrate solution is When using, according to the specific requirements of the experiment, perform relevant operations with the solution, and pay attention to the storage conditions of the solution to prevent its deterioration, so as to ensure the accuracy of the experimental results.