As a leading 2-(N-Morpholino)Ethanesulfonic Acid,Sodium Salt supplier, we deliver high-quality products across diverse grades to meet evolving needs, empowering global customers with safe, efficient, and compliant chemical solutions.
2- (N-Morpholino) Ethanesulfonic Acid, Sodium Salt What is the main use of
2-%28N-Morpholino%29Ethanesulfonic+Acid%2CSodium+Salt is 2- (N-morpholine) ethanesulfonate sodium salt, often referred to as MES sodium salt. Its main uses are as follows:
MES sodium salt is widely used in the field of biochemical research. First, it is effective in biological buffers. Biochemical reactions in organisms require a specific pH environment to proceed smoothly. MES sodium salt has good buffering ability and can maintain the stability of solution pH in a specific pH range (usually between pH 5.5 and 6.7). This property is crucial in enzymatic reactions, protein crystallization, cell culture and many other experiments. Taking cell culture as an example, cell growth requires a suitable and stable pH environment. MES sodium salt can create such conditions to ensure normal cell growth and metabolism, and avoid the adverse effects of pH fluctuations.
Second, it is also indispensable for the preparation of electrophoresis buffer. The purpose of electrophoresis experiments is to separate and analyze biological macromolecules, such as proteins, nucleic acids, etc. As a component of electrophoresis buffer, MES sodium salt can stabilize the pH value of the buffer system, ensuring that biological macromolecules can be effectively separated according to their own charges and sizes in the electric field. If the pH value is unstable, the charge state of biological macromolecules will change, which will affect the separation effect and the accuracy of experimental results.
Third, MES sodium salts are also useful in molecular biology experiments, such as the extraction and purification of DNA and RNA. It can help maintain the appropriate pH conditions of the reaction system, ensure the stability and integrity of nucleic acids, avoid nucleic acid degradation due to pH discomfort, and provide high-quality nucleic acid samples for subsequent molecular biology research, such as gene cloning, sequencing, etc.
What are the physical properties of 2- (N-Morpholino) Ethanesulfonic Acid, Sodium Salt
2-%28N-Morpholino%29Ethanesulfonic+Acid%2CSodium+Salt that is 2- (N-morpholine) ethanesulfonate sodium salt, the following is an ancient saying for you to describe its physical properties:
This agent is a white crystalline state, pure and uniform in quality. Its solubility is quite good, in water like fish get water, can form a homogeneous solution, but in common organic solvents, such as ethanol and ether, it is insoluble.
On its melting point, about 280 degrees Celsius, at this temperature, the crystalline state begins to change, and it looks like melting. Its stability is also considerable, and it can last for a long time without deterioration at room temperature and pressure. However, in case of strong acid and alkali, it may react, causing its nature to change.
As for hygroscopicity, it should not be underestimated. Exposed to high humidity air, it is easy to absorb moisture, resulting in gradual changes in its properties, or the risk of agglomeration. Therefore, when storing, it should be placed in a dry place to avoid moisture.
This agent is widely used in many fields, and its physical properties are the foundation of its effectiveness. It is related to the user's operation method and storage.
2- (N-Morpholino) Ethanesulfonic Acid, Sodium Salt What to pay attention to when storing
2-%28N-Morpholino%29Ethanesulfonic Acid% 2CSodium Salt is 2- (N-morpholino) ethanesulfonate sodium salt. When storing this substance, pay attention to the following things.
First, it should be placed in a cool place. If the temperature is too high, it may cause its chemical properties to change and affect the use effect. This is due to high temperature or can cause changes in the internal structure of the substance, such as chemical bond breaking or rearrangement, causing it to deteriorate.
Second, it should be stored in a dry place. Because it has a certain degree of hygroscopicity, if the environment is humid, it is easy to absorb water. Once it absorbs moisture, or causes it to agglomerate, it is unfavorable to use, and or due to the participation of water, it triggers unnecessary chemical reactions and destroys the purity of the substance.
Third, it needs to be sealed and stored. Oxygen, carbon dioxide and other gases in the air may react with the substance. Oxygen may cause oxidation, and carbon dioxide may participate in chemical reactions to change its composition.
Fourth, keep away from sources of ignition and strong oxidants. Although this substance is not flammable and explosive, it is dangerous in case of ignition or strong oxidants, or due to accidental reactions.
In short, proper storage of 2- (N-morpholino) ethanesulfonate sodium salt is essential to maintain its quality and performance. It is related to the subsequent use effect. The above requirements must be strictly followed when storing.
2- (N-Morpholino) Ethanesulfonic Acid, Sodium Salt
To prepare 2- (N - Morpholino) Ethanesulfonic Acid (2- (N - Morpholino) Ethanesulfonic Acid, Sodium Salt, referred to as MES sodium salt), the common method is as follows:
Take an appropriate amount of 2- (N - Morpholino) ethanesulfonic acid (MES acid) and place it in a clean container, and slowly add the sodium hydroxide solution according to the stoichiometric ratio. This process requires continuous stirring, because the reaction will generate heat, and the reaction temperature needs to be controlled to avoid overheating affecting the product. The temperature can be adjusted by ice bath or slow dropwise addition of sodium hydroxide solution.
The reaction formula is: MES acid + NaOH → MES sodium salt + H2O O.
Wait until the reaction is complete and the solution is neutral or near neutral (can be accurately monitored by pH test paper or pH meter, the target pH is about 7). After that, the reaction solution is distilled under reduced pressure to remove excess water. When a large number of crystals precipitate, stop distillation and cool to room temperature to allow the crystals to be fully analyzed.
Next, collect the precipitated crystals by filtration and wash them with an appropriate amount of cold organic solvent (such as ethanol) to remove impurities. After washing, place the crystals in a vacuum drying oven and dry them at a suitable temperature (such as 40-50 ° C) for a certain period of time until the crystals are constant weight. The resulting product is 2- (N-morpholino) ethanesulfonate sodium salt. The entire operation process needs to ensure a clean environment to avoid impurities from being mixed in and affecting the purity of the product.
What are the common reactions of 2- (N-Morpholino) Ethanesulfonic Acid, Sodium Salt and other compounds?
2-%28N-Morpholino%29Ethanesulfonic Acid, Sodium Salt is 2- (N-morpholine) ethanesulfonate sodium salt, often referred to as MES sodium salt. This substance is a commonly used buffer in biochemical research, and can maintain the pH stability of solutions in a specific pH range. Common reactions with other substances are as follows:
One is to react with acids. Because MES sodium salt is weakly basic, when encountering strong acids, nitrogen atoms on the morpholine ring will bind protons. For example, when reacting with hydrochloric acid, the chemical equation can be expressed as: $C_6H_ {12} NNaO_4S + HCl\ longrightarrow C_6H_ {13} NO_4S + NaCl $. After the reaction, 2- (N-morpholine) ethanesulfonic acid and sodium chloride are formed. This is a genus of acid-base neutralization, which can be used to adjust the pH of the solution.
The second is to react with metal ions. The sulfonate in the sodium salt of MES can complex with some metal ions, like with copper ions, to form a stable complex. This reaction may affect the chemical properties and activity of metal ions, which is crucial for some systems that need to control the activity of metal ions.
The third is to react with strong oxidants. The sodium salt has certain reducing properties. When exposed to strong oxidizing agents, the sulfonic acid group or morpholine ring may be oxidized, resulting in structural and property changes. For example, with hydrogen peroxide under certain conditions, the sulfonic acid group may be oxidized to a higher valence sulfur-containing group.
The fourth is to react with certain enzymes or proteins. In the biochemical environment, the MES sodium salt buffer system may interact with enzymes and proteins, or affect their activity and structure. Although it is not a chemical reaction, it has a great impact on biological experiments. When using it, it is necessary to consider the impact on biological macromolecules, select the appropriate buffer concentration and conditions, and ensure the stability of the experimental system.