What is the common Chinese name for 2- (N-Morpholino) Ethanesulphonic Acid?
The common Chinese name of 2-%28N-Morpholino%29Ethanesulphonic+Acid is 2- (N-morpholino) ethanesulfonic acid, often referred to as MES. This substance is commonly used in the field of biochemical research and belongs to a biological buffer.
MES is a white crystalline powder with good water solubility, and its pH buffering ability is relatively stable over a wide temperature and concentration range. In biochemical experiments, it is often used to maintain a specific pH environment to ensure the activity and stability of biomolecules. For example, in the separation, purification and analysis of biological macromolecules such as proteins and nucleic acids, MES can effectively control the pH value of the solution and avoid the impact of pH fluctuations on the structure and function of biological macromolecules. Due to its stable chemical properties and low toxicity to biological systems, MES is widely used in many biological experimental scenarios such as cell culture and enzyme reactions. Whether it is simulating the in vivo environment of organisms or providing suitable conditions for biochemical reactions, MES plays an important role in helping researchers obtain accurate and reliable experimental results.
What are the main uses of 2- (N-Morpholino) Ethanesulphonic Acid?
2-%28N-Morpholino%29Ethanesulphonic Acid, Chinese name 2- (N-) ethanesulfonic acid, is often used for MES. This substance has important uses in multiple fields such as biochemistry.
For biological and molecular biology, MES is often filled. Because of its specific pH, it can effectively maintain the pH value of the solution. Biochemical reactions such as enzyme reaction and protein analysis provide a specific acid environment to ensure that the activity and function of biological macromolecules are not affected by large pH waves.
MES also plays an important role in cell culture. The pH value of the cell environment is sensitive, and MES can improve the pH value of the cell culture base, so that it can meet the needs of cell production, promote normal cell production and proliferation, and prevent cell production or death due to pH insufficiency.
In the study of protein crystal production, it is important to control the pH value of the solution. MES acts as a key factor, which can precisely control the pH of the solution and help the protein to form a high amount of crystals, so as to analyze the three levels of protein through X-ray crystal production technology, and promote the development of biological research.
In addition, in some chemical synthesis reactions, if the reaction needs to be performed at a specific pH, MES can be used as a part of the reaction system to maintain the reaction environment and improve the reaction efficiency.
What are the chemical properties of 2- (N-Morpholino) Ethanesulphonic Acid?
2-%28N-Morpholino%29Ethanesulphonic Acid is 2- (N-morpholino) ethanesulfonic acid, often abbreviated as MES. Its chemical properties are unique, let me tell you one by one.
MES is a white crystalline powder, soluble in water, and widely used in the field of biochemical research. The first characteristic of this substance is that it has good buffering properties. It can effectively maintain the stability of the pH of the system within the pH range of 5.5-6.7. This property is crucial for many biochemical reactions in organisms. Because many biological enzymes are only active in a specific pH range, MES buffer can simulate the pH of the environment in organisms to ensure the smooth progress of enzymatic reactions.
Secondly, the chemical properties of MES are relatively stable, and it is not easy to react with common chemicals at room temperature and pressure. This allows it to maintain its own chemical structure and properties for a long time during storage and use. However, it should be noted that although its stability is good, it should also avoid contact with strong oxidants, strong bases and other substances to prevent chemical reactions.
Furthermore, MES biocompatibility is quite good. In biological experiments such as cell culture, MES buffers have no significant interference with cell growth and metabolism. This property allows researchers to use MES to create an environment suitable for cell growth and facilitate cell biology research. In conclusion, 2- (N-morpholino) ethanesulfonic acid occupies an important position in many scientific research fields such as biochemistry and cell biology due to its unique chemical properties such as buffering properties, stability and biocompatibility, providing a powerful tool for researchers to carry out various experiments.
2- (N-Morpholino) Ethanesulphonic Acid What should I pay attention to when storing?
2-%28N-Morpholino%29Ethanesulphonic Acid is 2- (N-morpholino) ethanesulfonic acid, often abbreviated as MES, which is a commonly used buffer in biochemical research. When storing, pay attention to the following numbers:
First, the control of temperature is very critical. MES should be stored in a low temperature and dry place. Generally speaking, it is recommended to store at a temperature between 2-8 ° C. If the temperature is too high, it may cause its chemical properties to be unstable and accelerate deterioration; if the temperature is too low, it may cause its crystallization to precipitate, which affects the use. If placed in a high temperature environment, it is easy to cause molecular structure changes, which in turn affects the buffering performance.
Second, moisture resistance cannot be ignored. Because of its certain water absorption, if it is damp, it will not only change its own concentration, but also may breed microorganisms and contaminate reagents. Therefore, it should be stored in a well-sealed container. After taking it, seal it in time.
Third, avoid light. Light may cause photochemical reactions to occur, which will adversely affect its structure and properties. It is best to store it in a brown bottle or in a dark place.
Fourth, isolate it from other substances. MES should avoid contact with strong oxidants, strong acids, strong bases and other substances to prevent chemical reactions from occurring and causing deterioration. When storing, it should be placed separately from such substances.
Fifth, check it regularly. Even if the correct storage conditions are followed, it is necessary to check its appearance, properties, etc. Once abnormal color changes or precipitation are found, it should be avoided.
What are the synthesis methods of 2- (N-Morpholino) Ethanesulphonic Acid?
2-%28N-Morpholino%29Ethanesulphonic Acid is 2- (N-morpholine) ethanesulfonic acid, often referred to as MES. Its synthesis method is as follows:
1. ** Morpholine and 2-chloroethanesulfonic acid are used as raw materials **:
- Take an appropriate amount of morpholine first, place it in the reactor, add an appropriate amount of organic solvent, such as ethanol or acetone, and stir to make the morpholine evenly dispersed in the solvent.
- Then slowly add 2-chloroethanesulfonic acid to it. This process requires strict control of the reaction temperature, usually maintained between 50-70 ° C, to avoid the occurrence of side reactions. During the dripping process, continue to stir to ensure that the two are fully in contact with the reaction.
- After the dropwise addition is completed, continue the reaction at this temperature for 3 - 5 hours. After the reaction is completed, the reaction liquid is cooled to room temperature, followed by reduced pressure distillation to remove the organic solvent.
- The remaining product is dissolved with an appropriate amount of water, and then purified by ion exchange resin to remove impurity ions. Finally, after concentration and crystallization, 2- (N-morpholino) ethanesulfonic acid can be obtained.
2. ** With morpholine and ethylene sulfonic acid as raw materials **:
- In the reaction vessel with a stirrer, thermometer and reflux condenser, add a certain amount of morpholine and an appropriate amount of catalyst, such as p-toluenesulfonic acid.
- Ethylene sulfonic acid gas is passed into it, and the reaction temperature is controlled at 40-60 ° C. During the process of passing ethylene sulfonic acid, continue to stir to fully react the gas with morpholine.
- After the reaction lasts for 2-4 hours, stop passing ethylene sulfonic acid and continue to stir for a period of time to ensure complete reaction. After that, add an appropriate amount of water to the reaction system to dilute the reaction solution.
- Adjust the pH value to neutral with sodium hydroxide solution, and then concentrate under reduced pressure to remove excess water. Then add an appropriate amount of organic solvent, such as ethyl ether or ethyl acetate, so that the product crystallizes and precipitates. After filtration and drying, 2- (N-morpholine) ethanesulfonic acid is obtained.