2- [Tris (Hydroxymethyl) Methyl] -2-Aminoethane Sulfonic Acid
2-%5BTris%28Hydroxymethyl%29Methyl%5D-2-Aminoethane Sulfonic Acid, Chinese name 2- (N-alkaline) ethanesulfonic acid, MES. Its main use is in the field of biochemical research, and its role is high.
MES is often used as a biological agent. The transformation and reaction of biological agents need to be carried out in a specific pH environment. Because of its good performance, MES can support the determination of pH and avoid adverse effects due to the function of pH waves and biomolecules. For example, in enzymatic reactions, the activity of enzymes is pH-sensitive, and MES can determine the pH of the environment, so that the enzyme can optimize its activity and promote the development of biochemical research.
Furthermore, in the field of cellular culture, MES is also indispensable. Cell growth requires specific pH components. MES can be used to prepare cell culture bases to ensure cell growth and proliferation in a pH environment, and to maintain normal physiological functions. It provides a good solution for cell biology research.
In addition, in the study of protein crystallization, MES can be used to control the pH of the solution to promote the precipitation of proteins in a specific crystal form, which is convenient for the analysis of three proteins by X-ray crystallography technology, helping to make breakthroughs in protein function research. Therefore, MES, with its unique properties, plays an important role in research fields such as polybiology and cellular biology.
2- [Tris (Hydroxymethyl) Methyl] -2-Aminoethane Sulfonic Acid is used in which fields
2-%5BTris%28Hydroxymethyl%29Methyl%5D-2-Aminoethane Sulfonic Acid, Chinese name 2- (N-) ethanesulfonic acid, is often used for MES. This substance is used in the field of biological and molecular biology.
In the field of biological and molecular biology, MES is often used for MES. Because of its specific pH, it can support biological pH determination between 5.5 and 6.7. Biology is sensitive to multi-enzymatic pH, and small pH waves may affect enzyme activity and even the reaction process. For example, in the analysis process of some specific proteins, the use of MES solution can protect the activity of the protein and avoid pH differences.
The cell culture field is also often found in MES. Plant cytogenetic environment is demanding, and pH determination is of paramount importance. MES can be used to prepare the cell culture base to maintain the appropriate pH environment and ensure the normal growth and proliferation of cells. For example, in some cell cultures, the MES culture base can provide the cell with a fixed external environment to help it grow well.
In addition, in analytical analysis, it involves the analysis and determination of biological products, such as high-performance liquid chromatography analysis of biomolecules. MES solution can be used in the fluid phase or pre-treatment of the product to improve the quality of the product and improve the reliability of the analytical results. In addition, MES plays an indispensable role in the multi-phase biological field due to its high pH performance.
2- [Tris (Hydroxymethyl) Methyl] -2-Aminoethane Sulfonic Acid
2 - [Tris (Hydroxymethyl) Methyl] - 2 - Aminoethane Sulfonic Acid, Chinese name "2 - (trimethylamino) ethanesulfonic acid", often used for TES. This is a commonly used method in biological and chemical research, and its chemical properties are specific, as follows.
First of all, its acid properties are described. TES has a specific pKa value, which is between 7.5 and 7.7 at 25 ° C. This property makes it effective at near-neutral pH, which can effectively maintain the pH value of the solution and avoid large pH fluctuations due to the addition of a small amount of acid or alcohol. In the study of biological chemistry, such as proteins and enzymes, the pH environment of this determination is of paramount importance. The activity and activity of proteins and enzymes often depend on a specific pH.
Furthermore, its solubility. TES is easily soluble in water, and this property is convenient for operation. It can be easily prepared into aqueous solutions of different degrees to meet different needs. And its solubility in water is good, which can ensure uniform dispersion in the solution and full effect.
It is also qualitative. The chemical properties of TES are well determined, and it is not easy to generate many chemical reactions under normal conditions. This qualitative guarantee guarantees that it can still maintain its own performance in the biochemical system, and will not lose its effectiveness due to the reaction of other components of the system.
In addition, TES has little impact on biological macromolecules. In biological research, this is a big problem. When using it as an important function, it is generally not affected by the function of biological macromolecules such as proteins and nucleic acids, so it can make the results more reliable and truly reflect the original characteristics of biological macromolecules.
2- [Tris (Hydroxymethyl) Methyl] -2-Aminoethane Sulfonic Acid
2 - [Tris (Hydroxymethyl) Methyl] - 2 - Aminoethane Sulfonic Acid, Chinese name 2 - (trimethylamino) ethanesulfonic acid, often used for TES. This is commonly used in biological molecular biology, and has many physical properties.
First of all, its solubility, TES is easily soluble in water, which makes it easy to dissolve in aqueous solutions. It can be quickly dispersed and easily operated. And its solubility in water can effectively hold the solution, and it is not easy to form sedimentation or other chemical reactions, providing a reliable environment.
Re-evaluate its ability, and the performance of TES is roughly between pH 6.8 and 8.2. At this pH level, it can effectively resist the pH change caused by the addition of a small amount of acid or acid, and maintain the pH value of the solution. This property is very important for polybiodization, because the activity of polymolecules depends highly on a specific pH. TES can create a micro-environment of reaction and synthesis, and ensure the operation of reaction.
Also known as its melting temperature, it has a high melting temperature, which means that under normal conditions, TES is fixed, which is easy to preserve. And in general, it is not easy to decompose or decompose due to the increase in temperature, which further increases the reliability of its use.
In addition, the chemical properties of TES are not stable, and it is not easy to generate reaction of many normal chemicals. This makes it compatible with multiple biomolecules, enzymes, and other organisms, and can also function well in biochemical systems without interfering with other chemical reactions or biological processes.
2- [Tris (Hydroxymethyl) Methyl] -2-Aminoethane Sulfonic Acid
The method for preparing 2 - [Tris (Hydroxymethyl) Methyl] - 2 - Aminoethane Sulfonic Acid (referred to as TES) is described in detail as follows:
First take an appropriate amount of trihydroxymethylaminomethane (Tris) and place it in a clean reaction vessel. This is the starting material of the reaction, and it needs to be of high quality and high purity to be the foundation for a smooth reaction.
Add 2 - bromoethanesulfonic acid (2 - bromoethanesulfonic acid) dropwise to the above container containing Tris. The rate of dropwise addition should not be too fast to prevent the reaction from being overheated and losing control. In this process, the temperature should be strictly controlled to maintain a suitable reaction temperature. This temperature setting is related to the reaction rate and product purity. Generally speaking, it should be controlled within a specific temperature range to make the reaction proceed in the expected direction.
And when reacting, an appropriate amount of alkali, such as potassium carbonate, needs to be added to promote the reaction. The amount of alkali needs to be accurately measured, and too much or too little can cause the reaction to be biased.
When the reaction continues, it should be stirred continuously to make the reactants fully contact each other, so that the reaction is more uniform and efficient. The speed of stirring also needs to be appropriate. Too fast or too slow can affect the reaction effect.
After the reaction is completed, the reaction mixture is subjected to vacuum distillation to remove the solvent and unreacted raw materials. This step aims to preliminarily purify the product and lay the foundation for subsequent refining.
Then, the distilled product is recrystallized with an appropriate solvent. The choice of solvent is quite critical, and its solubility to the product varies significantly with temperature. In this way, by recrystallization, impurities can be effectively removed and the purity of the product can be improved. After several recrystallization, high purity 2 - [Tris (Hydroxymethyl) Methyl] - 2 - Aminoethane Sulfonic Acid can be obtained.
Every step of operation requires fine and careful control, and the control of each parameter is related to the quality and quantity of the product. Only by following this rigorous method can high-quality TES be obtained.