What are the chemical properties of 2- [Tris (Hydroxymethyl) Methyl] Amino] Ethanesulfonic Acid
2-%5BTris%28Hydroxymethyl%29Methyl%5DAmino%5DEthanesulfonic Acid, Chinese name 2- [tris (methyl) methylamino] ethanesulfonic acid, is often used for TES. Its chemical properties are unique, and it is widely used in biochemical research and other fields.
This compound is a white crystalline powder. In terms of acid properties, it is a good solution, and has the ability to determine the phase. The pKa value of TES is between 7.5 and 7.7, which makes it effective in maintaining the pH value of the solution at a pH close to neutral. In biochemistry, such a pH environment is essential for the activity of many biological macromolecules, such as proteins, enzymes, etc. It is usually at a specific pH to exhibit the best activity, and the TES solution can provide a suitable environment.
TES has good water solubility and can be miscible in water in any ratio, which is convenient for researchers to operate in liquids of different degrees. Moreover, its chemistries are not easy to react due to the interaction of other substances. In addition, the toxicity of TES is low, and in the system involving organisms such as cell culture, it is not caused by normal physiological activities such as cell production and generation. Therefore, it can be used as a component in the cell culture solution.
2- [Tris (Hydroxymethyl) Methyl] Amino] Ethanesulfonic Acid is commonly used in which experiments
2-%5BTris%28Hydroxymethyl%29Methyl%5DAmino%5DEthanesulfonic Acid, Chinese name 2- "tris (methyl) methylamino" ethanesulfonic acid, is often used to represent TES. This compound is used in many applications, as described below.
In biological chemistry, it is often used as a chemical. Biological chemistry is sensitive to acidity, and small pH fluctuations may affect the activity of enzymes and the function of proteins. Due to its specific pKa value, TES can effectively support the activity of enzymes at a certain pH. For example, in the study of some specific enzymatic reactions, it is necessary to carefully control the pH environment. TES can be used to make enzymes under the most suitable catalytic components, so as to ensure the effectiveness of enzymes.
Cell culture is also a common place for TES. The pH requirements of the environment in which cells are produced are harsh, and inappropriate pH values can inhibit cell proliferation and even cause cell death. Adding TES to the culture base of cells can help maintain a certain pH environment, provide good components for cell generation and generation, and ensure the normal physiological functions of cells.
In addition, in molecular biology, such as DNA reprocessing technology, polymerase reaction (PCR) and other processes, there is also no shortage of TES. These reactions need to be performed efficiently and effectively under specific pH conditions. TES can be used as a component to ensure the pH determination of the system, and help the operation of biological macromolecules to complete the analysis.
Therefore, 2-" tris (methyl) methylamino "ethanesulfonic acid, with its excellent performance, plays a crucial role in many fields such as biochemistry, cell culture, and molecular biology, providing a reliable and reliable environment for researchers' research.
2- [Tris (Hydroxymethyl) Methyl] Amino] Ethanesulfonic Acid
2 - [Tris (Hydroxymethyl) Methyl] Amino Ethanesulfonic Acid, that is, 2 - (N - tris (hydroxymethyl) methylamino) ethanesulfonic acid, often referred to as TES. This substance is a commonly used buffer in biochemical experiments. In order to ensure its quality and performance, it is essential to store it correctly.
It is advisable to store TES in a dry place. Because humidity can affect its stability, if the environment is humid, it may cause it to absorb moisture, which may change its chemical properties and reduce its buffering capacity.
Temperature is also a key factor. It usually needs to be placed in a cool place. Generally, the recommended storage temperature is 2-8 ° C. If the temperature is too high, it may cause a chemical reaction and cause it to deteriorate; if the temperature is too low, although it does not often cause it to freeze, it may affect its crystal structure and have an adverse effect on its solubility and buffering effect.
In addition, it should be avoided from contact with strong oxidants, strong acids, strong bases and other substances during storage. Because of the presence of active groups such as amino groups and sulfonic acid groups in its chemical structure, it is easy to react with the above substances, thereby destroying its structure and reducing its buffering performance.
Containers for storing TES should be made of well-sealed materials, such as glass bottles or high-quality plastic bottles. Glass bottles are chemically stable and will not react with TES; high-quality plastic bottles have good sealing and corrosion resistance, which can effectively prevent their leakage and deterioration.
In short, the correct storage of 2 - [Tris (Hydroxymethyl) Methyl] Amino Ethanesulfonic Acid requires attention to environmental humidity and temperature, avoid contact with incompatible substances, and choose a suitable storage container, so as to ensure that it plays a good buffering role in the experiment.
What is the pH Buffer Range of 2- [Tris (Hydroxymethyl) Methyl] Amino] Ethanesulfonic Acid
2 - [Tris (Hydroxymethyl) Methyl] Amino] Ethanesulfonic Acid, Chinese name 2 - (N - tris (methyl) methylamino) ethanesulfonic acid, often used for TES. This is commonly used in biochemical research.
Its pH is generally between 6.8 and 8.2. At this pH level, TES can effectively maintain the pH value of the solution and resist the pH reduction caused by the addition of a small amount of acid or water. The reason for this is due to the chemical properties of TES. There are soluble bases in its molecules. At a specific pH level, these groups can react to the H or OH levels in the solution, and the pH value of the solution can be determined.
In many fields of biochemical research, such as protein research, enzyme reaction, etc., because the activity of protein enzymes is often sensitive to pH value, it is necessary to precisely control the pH environment. The pH of TES meets the needs of many biochemical reactions, and can provide fixed pH components to ensure the reliability and reliability of results.
2- [Tris (Hydroxymethyl) Methyl] Amino] Ethanesulfonic Acid
To prepare 2 - [Tris (Hydroxymethyl) Methyl] Amino] Ethanesulfonic Acid (referred to as TES), the common method is as follows:
First, use trimethylol aminomethane (Tris) and 1,2 - ethane sulfonate lactone as raw materials. In a suitable reaction vessel, accurately measure an appropriate amount of Tris and dissolve it in water or an organic solvent to form a uniform solution. Then add 1,2 - ethane sulfonate lactone slowly according to stoichiometry. When adding, be sure to pay attention to the reaction temperature, which is usually controlled in the low temperature range, generally about 0 - 10 ° C, to prevent side reactions from breeding.
Add it up, gradually heat up to a moderate temperature, usually 30 - 50 ° C, and continue to stir the reaction at this temperature. The reaction time varies depending on the proportion of raw materials and reaction conditions, roughly between a few hours or even ten hours. During the reaction process, thin layer chromatography (TLC) or high performance liquid chromatography (HPLC) can be used to monitor. When the raw material Tris is significantly reduced and the amount of product TES is stabilized, the reaction can be regarded as basically completed.
After that, the reaction solution needs to be treated. First, adjust the pH value of the reaction solution to a suitable range with an appropriate acid-base regulator to separate the product. Then, the solvent is removed by vacuum distillation or rotary evaporation. The residue can be purified by recrystallization or column chromatography to obtain a pure TES product. During recrystallization, select a suitable solvent, such as ethanol-water mixed solvent, heat to dissolve the residue, and then slowly cool down, so that TES crystallization precipitates, suction filtration, drying, to obtain the finished product. Column chromatography selects the appropriate fixed phase and mobile phase according to the polarity difference between the product and the impurity, in order to achieve the purpose of separation and purification.