2- [Tris (Hydroxymethyl) Methylamino] -1-Ethanesulfonic Acid
2-%5BTris%28Hydroxymethyl%29Methylamino%5D-1-Ethanesulfonic Acid, Chinese name 2- [tris (methyl) methylamino] ethanesulfonic acid, is often used to control TES. Its main use is often used in biochemical research and in room-like situations.
In biological molecular biology, TES is often used for high performance. Because of its specific characteristics, it can effectively maintain the pH value of the solution, so that the biochemical reaction can be carried out in a suitable acid environment. Where protein extraction, enzyme activity research, or nucleic acid phase is used to control the pH value, TES can play an important role. The function of biochemical molecules is sensitive to pH value, and a slight deviation may lead to adverse effects such as protein resistance and enzyme activity loss.
Furthermore, in the field of cell culture, TES is also useful. The generation of cells requires a certain environment, in which pH is an important factor. TES can be used to prepare cell culture bases to ensure that cells can grow and multiply normally at an appropriate pH, so as to avoid damage to cells due to pH waves.
In addition, in some chemical research and development processes, TES may also be used as a aid, used to improve the pH value of the chemical solution, improve the qualitative and bioavailability of the chemical, and help the chemical to be more effective.
2- [Tris (Hydroxymethyl) Methylamino] -1-Ethanesulfonic Acid What is the purity standard?
2-%5BTris%28Hydroxymethyl%29Methylamino%5D-1-Ethanesulfonic Acid, or TES, is a commonly used buffer in biochemical research. Its purity standard is quite critical, which is related to the accuracy and reliability of the experiment.
Under the text of "Tiangong Kaiwu", the purity standard of TES needs to be considered in many aspects. The purity of the first chemical composition. If impurities exist, they will mess up the image of the experiment. In an ideal state, the content of its main components should be extremely high, almost pure and beautiful, such as fine gold and jade, with less impurity disturbance.
Furthermore, physical properties are also important. The appearance should be uniform and stable, the color should be pure, and there should be no variegated colors. The particle size distribution should be uniform, so that it can be quickly soluble and uniform when dissolved and other operations, such as fine sand entering water, instant fusion.
Solubility is also the key. In commonly used solvents, it should be able to dissolve rapidly and completely without precipitation residue, like ice and snow in the warm sun, completely melting.
Moisture content is also determined. Excessive moisture can easily cause deterioration and affect the buffering performance. Therefore, it is necessary to strictly control and maintain an appropriate range, such as seedlings moisturizing the soil and moderate moisture.
The method of purity detection should be accurate and reliable. With modern instrument analysis, such as high-performance liquid chromatography, mass spectrometry, etc., the proportion of impurities and main components can be measured in detail. The process of operation requires rigor like the ancient method of casting swords, and it is necessary to investigate every detail.
All these are the essentials of 2-%5BTris%28Hydroxymethyl%29Methylamino%5D-1-Ethanesulfonic Acid purity standards to ensure the smoothness of the experiment and the certainty of the results.
2- [Tris (Hydroxymethyl) Methylamino] -1-Ethanesulfonic Acid What are the precautions when storing
2 - [Tris (Hydroxymethyl) Methylamino] - 1 - Ethanesulfonic Acid, Chinese name, tris (methyl) methylaminoethanesulfonic acid, often used for TES. If this material is stored, it is necessary to pay attention to it.
First, the degree is very important. It is suitable for low dryness, and it is usually recommended to be placed in a refrigerated environment of 2-8 ° C. If the degree is high, it may cause changes in its chemical properties, causing it to lose its original effect. For example, high temperature may cause its molecules to dissolve, affecting its ability to reduce pH in biochemistry.
Furthermore, pay attention to moisture resistance. This material is easy to absorb moisture. Once it is damp, it is impossible to produce an image, and it will be reversed due to water intervention, breaking its temperature. Therefore, it should be stored in a well-sealed container to avoid moisture and empty.
In addition, it should not be ignored when protected from light. Light may cause actinic reactions, making the nature of TES uncertain. Therefore, it is best to store it in a brown bottle or a container with a shading function to prevent light from causing damage.
The same, the storage needs to be oxidized, acid and other chemical substances. Due to the chemical properties of TES, it is easy to cause some substances to react, which affects their products. For example, oxidation, or strong reaction, such as oxidation.
Therefore, proper storage of 2 - [Tris (Hydroxymethyl) Methylamino] - 1 - Ethanesulfonic Acid requires more attention in terms of temperature, moisture, light, and other chemical barriers, so as to ensure its chemical properties and long-term effectiveness in high temperature and related applications.
2- [Tris (Hydroxymethyl) Methylamino] -1-Ethanesulfonic Acid
2-%5BTris%28Hydroxymethyl%29Methylamino%5D-1-Ethanesulfonic Acid, often used to produce TES, is commonly used in biological chemistry. The correct method of use is very important, and the following is generally necessary.
First, the solution should be prepared. The required degree of TES must be calculated according to the required degree of TES. If you want to prepare a specific degree of TES solution, you can take a large amount of TES powder and dissolve it in water. Dissolve it, or need to be mixed, to promote its instant dissolution.
The pH value of TES is low. The temperature of TES is between pH 6.8 and 8.2. To determine the required pH value, you can add acid or solution slowly, and use the refined pH. Do not worry about it to prevent the pH value from being biased.
Furthermore, consider the degree of solution. The degree of solution is very high, the water used must be high-grade water, and the utensils used also need to be cleaned and sterilized to avoid dry fruit.
In addition, the quality of the solution should be determined. The TES solution should be prepared for use. If it needs to be stored, it should be placed in a low temperature and protected from light, and its pH value and microbial contamination should be checked regularly.
At the end of use, it is necessary to follow the safe procedure of the room when using it. If you accidentally connect the skin or eyes, wash it with a lot of water immediately, and it is necessary to ask for treatment. In this way, you can use 2-%5BTris%28Hydroxymethyl%29Methylamino%5D-1-Ethanesulfonic Acid properly to ensure the safety of the product.
2- [Tris (Hydroxymethyl) Methylamino] -1-Ethanesulfonic Acid Reaction contraindicated with other chemicals
2 - [Tris (Hydroxymethyl) Methylamino] - 1 - Ethanesulfonic Acid, known as TES, is commonly used in biochemical research. Its properties are determined by the phase, and usually the polymers are less likely to react, but there are also specific situations that need to be paid attention to.
In common biochemical terms, the synthesis of TES-gold particles is weak, and generally no sedimentation or complex is formed. In the case of gold catalysis, the reaction is very small. The oxidation of TES meets, because TES has a certain originality, or the oxidation reaction occurs, resulting in the change of its performance. In the same way, if it encounters the original reaction, or the reaction, it will affect its own performance.
In the field of protein and nucleic acid research, TES liquid can maintain the pH environment, do not affect the biological characteristics of proteins and nucleic acids, and ensure the function of biological macromolecules.
In the enzymatic reaction system, under multiple conditions, TES does not inhibit enzyme activity, and can provide pH components. However, for some environment-sensitive enzymes, TES may have a slight impact on enzyme activity due to factors such as temperature, so it needs to be checked.
For example, 2 - [Tris (Hydroxymethyl) Methylamino] -1 - Ethanesulfonic Acid generally has less reaction to polymers, and is used for multiple biochemical reactions. However, in the system, it is necessary to consider its compatibility according to the physical properties and anti-demand, and to ensure the profitability of the system.