Products

2-(Tris(Hydroxymethyl)Methylamino)Ethane-1-Sulphonic Acid

Lingxian Chemical

Specifications

HS Code

664908

Chemical Formula C6H15NO6S
Molar Mass 229.25 g/mol
Appearance White crystalline powder
Solubility Soluble in water
Pka 7.5 - 7.7
Ph Range For Buffer 6.8 - 8.2
Melting Point 190 - 195 °C
Density 1.39 g/cm³
Stability Stable under normal conditions
Hazard Class Non - hazardous for most common uses
Packing & Storage
Packing 100g of 2-(Tris(Hydroxymethyl)Methylamino)Ethane - 1 - Sulphonic Acid in resealable chemical - grade pouch.
Storage 2-(Tris(Hydroxymethyl)Methylamino)Ethane - 1 - Sulphonic Acid should be stored in a cool, dry place away from heat and sources of ignition. Keep it in a tightly sealed container to prevent moisture absorption. Avoid storing near incompatible substances. Ideal storage temperature is around room temperature (15 - 30°C). This preserves its chemical integrity for optimal use in laboratory or industrial applications.
Shipping 2-(Tris(Hydroxymethyl)Methylamino)Ethane - 1 - Sulphonic Acid should be shipped in well - sealed, corrosion - resistant containers. Ensure proper labeling indicating its chemical nature. Transport under conditions that avoid exposure to moisture and extreme temperatures.
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2-(Tris(Hydroxymethyl)Methylamino)Ethane-1-Sulphonic Acid
General Information
Historical Development
The origin of 2 - (tris (hydroxymethyl) methylamino) ethane - 1 - sulfonic acid can also be investigated. In the past, various sages worked tirelessly in the field of chemistry. At first, I never heard of this thing. Later, after the efforts of many scholars, in the back and forth of experiments and exploration, there are gradually clues. From ignorance to clarity of its nature and understanding of its system. From the beginning of its creation, to today's application, during which it has been tossed and turned, step by step difficult. However, due to the unswerving determination of everyone, this 2 - (tris (hydroxymethyl) methylamino) ethane - 1 - sulfonic acid has emerged in the chemical industry, and its use has become increasingly widespread. It is also important in the academic and industrial circles.
Product Overview
Today there is a thing called 2- (Tris (hydroxymethyl) methylamino) ethane-1-sulfonic acid. This is a delicate chemical with a wide range of uses. Its properties are unique, and it has its own state and characteristics. In the field of experimental investigation, it is often used as a buffer, which can stabilize the pH of the system, just like the needle of the sea, making the reaction environment quiet and stable. In many aspects of biochemical research, it plays a key role, helping researchers to understand the mysteries of the microscopic. With its characteristics, it can regulate many reaction processes and ensure the accuracy of experiments. It is an indispensable product in the field of chemical research. It has made great contributions to exploring the unknown and revealing the truth. It is also a good aid for scientific research.
Physical & Chemical Properties
2 - (Tris (Hydroxymethyl) Methylamino) Ethane - 1 - Sulphonic Acid, this substance is quite specific in nature. Its shape or crystalline, pure and moist in color, soluble in water, when dissolved, silent change, liquid clear and uniform. Its heat is quite self-sustaining, under high temperature, the structure is still stable, not rapid change. Regarding its chemical properties, it can react with alkalis to form a genus of salts, and in many reactions, it can be used as a buffering agent to balance the acid and base of the system without sudden change. Such physical properties and chemical properties are of great use in the research of biochemistry, the preparation of pharmaceuticals, etc., and are also important things that cannot be ignored.
Technical Specifications & Labeling
Today there is a thing named 2- (Tris (Hydroxymethyl) Methylamino) Ethane - 1 - Sulphonic Acid. Its technical specifications and identification (product parameters) should be reviewed in detail. Its quality needs to meet specific regulations, with pure color, stable sex, few impurities and accurate content. The marker should indicate its name, show its nature, and mark its parameters, so that everyone can distinguish it. Those who produce this must follow precise methods, control temperature, speed, monitor quality, and follow the rules, so as to obtain a good product. At the time of inspection, the standard shall prevail, measure its degree and test its performance, and there will be no error before it can be released. In this way, the quality of this product can be guaranteed, and it can be adapted to all uses.
Preparation Method
The method of preparing 2 - (tris (hydroxymethyl) methylamino) ethane - 1 - sulfonic acid is as follows: Prepare the required raw materials, take an appropriate amount of starting materials, and proceed according to the specific production process. Start with a certain reaction step, carefully control the temperature, pressure and reaction time conditions. Such as the interaction of starting materials, through delicate chemical reactions, gradually transformed. During this period, close attention should be paid to the reaction process, and the conditions should be adjusted in a timely manner according to the characteristics of the reaction. When the reaction reaches a specific stage, purification is carried out. A variety of purification methods, such as filtration, crystallization, chromatography, etc., can be used to remove impurities and improve the purity of the product. In this way, after many steps and rigorous operation, high purity 2 - (tris (hydroxymethyl) methylamino) ethane - 1 - sulfonic acid products can be obtained.
Chemical Reactions & Modifications
Today there is a thing called 2- (Tris (Hydroxymethyl) Methylamino) Ethane - 1 - Sulphonic Acid. The reaction and modification of the chemical is what we are studying. Its properties are in the chemical process, or there are some unsatisfactory aspects, we should think about changing it.
Looking at the chemical process, or the harshness of the conditions, or the small yield, are all points that can be changed. To seek the excellence of its properties, we must investigate the mechanism of the response in detail, and explore the change of its bond and the change of energy. If the conditions are not suitable, adjust the temperature, pressure, and amount of agent to make it go smoothly. In the way of modification, either introduce other groups, or change its structure, in the hope of better properties, for all kinds of use. Although the road ahead may be difficult, those of us who study chemistry must be exhausted, seeking the wonders of materialization and modification to benefit the world.
Synonyms & Product Names
2 - (tris (hydroxymethyl) methylamino) ethane - 1 - sulfonic acid, this substance is very important in biochemical research. It has many other names and is also well known in the industry. Its function is remarkable. In a specific reaction system, it can effectively regulate the pH and maintain the stability of the system. It is just like the boat that stabilized the rudder in ancient times to ensure smooth navigation.
From the perspective of characteristics, it has good solubility and buffering ability, just like the ancient sages, it can accommodate all parties and reconcile things. In many experiments and production processes, with its unique chemical structure, it plays a key role, just like the ancient art of war, with exquisite layout to win thousands of miles. Its trade names are also diverse, with different uses and sources in the industry, each with its own name, but they all refer to the same divine substance, which together contribute to scientific research and production.
Safety & Operational Standards
Specifications for the Safety and Operation of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1 - Sulphonic Acid
Fu 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1 - Sulphonic Acid, a chemical product. To understand its safe operation, how to study it.
The first word is safety. This object should be stored in the room. It should be dry and clear. It should be a source of fire and oil, so as not to cause danger. If it is hard to see, quickly wash it with plenty of water. If you don't feel it, ask for it. If it enters the eye, wash it immediately with physiological water or water. If it cannot be delayed, it will be treated. If you accidentally eat it, do not urge vomiting, and send it to the hospital quickly.
Next operation. When operating, wear and prevent equipment, such as gloves, gloves, etc., to avoid body damage. When preparing the solution, according to the proportion of fine, dissolve it into the solution, and do not mix it, so that it is homogeneous. Use it, store it properly, clean the equipment, and do not leave it.
Therefore, for the research and use of 2- (Tris (Hydroxymethyl) Methylamino) Ethane - 1 - Sulphonic Acid, it is necessary to follow the safe operation, so that the risk can be avoided before it occurs, so that the research can be carried out.
Application Area
2 - (tris (hydroxymethyl) methylamino) ethane - 1 - sulfonic acid is a wonder of chemistry. Its use is quite extensive, in the field of biochemical research, often used as a buffer. In the field of cell culture, it can stabilize the pH of the environment, make cells grow safely, such as a quiet harbor, and protect the integrity of cells. In the study of proteins, it helps proteins maintain their inherent state, just like skilled craftsmen, maintaining both their form and spirit. In the process of enzymatic reactions, it creates suitable acid-base conditions, just like a pilot light, which promotes the reaction to run unimpeded. From this point of view, this chemical substance has an indispensable position in many application fields, and it is actually the right assistant of the scientific research journey.
Research & Development
Recently, a chemical product has been developed, which is called 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1 - Sulphonic Acid. We have dedicated ourselves to studying its characteristics and exploring its uses.
At the beginning, in the conditions of compatibility and reaction of various raw materials, we have repeatedly inferred, hoping to obtain a good method. After several years, the test has been frequent, or failed or succeeded, and there has been no slack. Gradually, this product can stabilize the pH of the solution in biochemical experiments, and its effect is outstanding.
Looking at its development now, the prospect is quite good. With the advance of biochemical technology, its demand will grow day by day. We should continue to refine our research, seeking the goodness of craftsmanship and the improvement of quality, hoping that this product can be widely used in the world, adding to the biochemical industry, promoting the prosperity of science and technology, and living up to the heart of research.
Toxicity Research
Today there is a thing called 2- (Tris (Hydroxymethyl) Methylamino) Ethane - 1 - Sulphonic Acid. As a chemical researcher, I explore its toxicity. The toxicity of this thing is related to the safety of all living beings and must not be ignored.
The process of research is carefully and cautiously. Observe its response to various things, and observe its effect on living beings. After repeated experiments, record the results in detail. See that it may have an effect on microorganisms under specific conditions. Although the poison of evil has not been found, it should not be ignored.
The study of toxicity is to ensure the harmony of all things. When using this thing in the future, you should follow what you get and do it carefully. So that the benefits can be recognized, and the harm can be avoided, it will live up to the heart of the researcher, and it will be in line with the way of heaven and earth.
Future Prospects
Today there is a thing called "2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid". Our generation studied it chemically to see its future prospects, and I had some thoughts.
This material is strange and has a wide range of uses. In the field of biochemistry, it can be used as a buffer agent to stabilize the acid and alkali of the system. Looking at today's world, biochemistry has developed rapidly, and scientific research depends on this agent.
Looking to the future, science and technology are changing day by day. This substance may make great progress in the development of medicine, help new drugs become successful, and solve people's diseases. Or in the science of materials, it can make great achievements, create new materials, and meet the needs of all parties. Our chemical researchers, when dedicated to exploring its potential, hope that the material in the unfinished time, shine, for the well-being of the world, into the future of the grand cause.
Frequently Asked Questions
What are the main uses of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid
2-%28Tris%28Hydroxymethyl%29Methylamino%29Ethane - 1 - Sulphonic Acid, Chinese name 2 - (tris (methyl) methylamino) ethanesulfonic acid, TES. This is a commonly used method in biological molecular biology, and its main uses are as follows:
First, it can determine the pH of the solution. Biology is sensitive to the pH value of multiple biochemical reactions, and it may affect the activity of enzymes, proteins, and biomolecules. TES has good performance and can effectively control the pH value of the solution, so that it can be kept at a specific level, so as to protect the biochemical reaction. For example, in some enzymatic reactions, a specific pH is necessary for the activity of enzymes, and TES can be used to maintain this environment.
Second, it can be used for protein research. In the study of protein extraction, determination and function, it is necessary to determine the pH of proteins to prevent protein accumulation or aggregation. TES solution can provide a suitable environment to help proteins maintain their natural image activity, so that scientists can deeply explore their characteristics.
Third, nucleic acid research is also commonly used. Nucleic acid extraction, PCR amplification and swimming analysis, etc., all require specific pH components to ensure the efficiency of nucleic acid characterization and reaction. TES solution can be used to determine the pH value of both nucleic acid and nucleic acid-holding phase, making the results more reliable.
Therefore, 2 - (tris (methyl) methylamino) ethanesulfonic acid, by virtue of its pH-determining properties, plays a crucial role in the operation of biological chemistry, molecular biology, and related fields, providing a powerful tool for researchers to explore the secrets of biomolecules.
What are the advantages of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid in biological experiments
2 - (tris (hydroxymethyl) methylamino) ethane-1 -sulfonic acid, often called TES, has many advantages in biological experiments.
First, this substance has good buffering properties. Within a specific pH range, it can effectively resist the influence of adding a small amount of acid, base or moderate dilution, so that the pH of the system remains relatively stable. In many biochemical reactions, the activity of enzymes and the structure and function of proteins are extremely sensitive to pH. For example, when cells are cultured, the appropriate pH is the key to normal cell growth and metabolism. TES buffer can create and maintain such a suitable environment to ensure the stable development of cell experiments.
Second, the chemical properties of TES are stable. Under normal experimental conditions, it is not easy to chemically react with other substances in the system, and does not interfere with the experimental process and results. This is particularly important in complex biological system experiments, because it can ensure the stability of the buffer itself, and will not react with reactants, products or other reagents to generate interfering by-products, thus ensuring accurate and reliable experimental results.
Third, it has good compatibility with biomolecules. It has little effect on the structure and function of biological macromolecules such as proteins and nucleic acids, and will not cause denaturation or inactivation of biomolecules. In experiments such as protein purification and enzyme activity determination, it can provide a stable environment for biomolecules without changing their inherent characteristics and activities, which is helpful for obtaining true and accurate experimental data.
Fourth, good solubility. It is easily soluble in water and can conveniently configure buffers of different concentrations to meet various experimental needs. And the dissolution process is fast, which can quickly form a uniform and stable solution, which brings many conveniences to the experimental operation and improves the experimental efficiency.
What is the optimal concentration range for 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid?
2-%28Tris%28Hydroxymethyl%29Methylamino%29Ethane - 1 - Sulphonic Acid, Chinese name tris (methyl) methylaminoethanesulfonic acid, TES. The optimum degree of use varies depending on the needs and purposes.
In protein research, it is often used at a degree of 0.05 - 0.1M. Because of this degree of stability, it can effectively maintain the activity of the protein, and can reduce the acidity of the solution, so that it can be maintained at the appropriate pH of the protein.
If used in cell culture, the degree of resistance is usually 0.01 - 0.05M. This degree can not only provide the required normalization environment for cell production, but also do not cause adverse effects on cell transmission, etc., to ensure the normal production of cells.
However, in some enzymes, the optimum degree of resistance may fall within 0.1-0.2M. In this case, it can be used to effectively counteract the pH waves caused by factors such as substitution generation in the reaction process, so that the enzyme can develop high-efficiency catalytic activity at the most appropriate pH.
Therefore, the optimum degree of use of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1 - Sulphonic Acid can only be determined according to factors such as resistance and research.
What is the stability of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid?
2 - (tris (hydroxymethyl) methylamino) ethane-1 -sulfonic acid, often referred to as TES, has good stability.
This substance contains a special functional group structure and has a certain chemical stability. The sulfonic acid group (-SOH) is stable and can enhance the stability of the molecule in solution. The sulfonic acid group is a strong acidic group, which is not easy to be oxidized or reduced under common conditions, and the structure is stable.
From the perspective of the molecule as a whole, the tris (hydroxymethyl) methylamino part is an amino group connected to multiple hydroxymethyl groups. Hydroxymethyl has a certain electron donor effect, which can increase the density of the amino electron cloud, enhance the interaction with the sulfonic acid group, and improve the overall stability of the molecule.
TES is chemically stable and can maintain its own structural integrity in aqueous solution. In a wide pH range (about pH 6.0 - 8.5), it has good buffering performance and the structure is not easily damaged. And it is inert to most common chemical reagents. Under ordinary oxidation, reduction, acid-base reaction conditions, its molecular structure is stable. However, under extreme conditions such as high temperature and strong oxidants, the structure may be affected.
In addition, TES has good stability in solid state. If stored in a dry and cool environment, avoid light and moisture, it can maintain its own structure and properties for a long time, and is not prone to decomposition and deterioration.
Characteristics of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid Compared to Other Buffers
2 - (tris (hydroxymethyl) methylamino) ethane - 1 - sulfonic acid, often abbreviated as TES, has the following characteristics compared with other buffers:
First, it exhibits buffering advantages in a specific pH range. The effective buffering range of TES is about pH 6.8 - 8.2, which is suitable for many biochemical and molecular biology experiments. Compared with phosphate buffer, its buffer range is narrow but the pH is precisely regulated in the neutral alkali region. In enzymatic reactions such as enzymatic reactions that need to maintain the stability of this pH range, it can prevent the enzyme activity from being affected by pH fluctuations.
Second, the chemical stability is quite good. The molecular structure of TES makes it difficult to decompose or have side reactions with other substances under common experimental conditions. Like Tris buffer at high temperature or in the presence of some metal ions, chemical reactions may occur to affect the buffering effect, while TES has strong stability and can stably maintain pH in biochemical systems containing metal ions or experiments that require high temperature treatment of samples.
Third, it has little interference with biological systems. In biological experiments such as cell culture and protein purification, many buffers may interact with biomolecules and interfere with experimental results. TES charge distribution and molecular size make it weakly interact with biological macromolecules such as proteins and nucleic acids, and do not interfere with the activity and structure of biomolecules, which is conducive to obtaining accurate experimental data.
Fourth, good solubility. Whether it is in water or common organic solvents, TES can be well dissolved, which is convenient for the experimental configuration of buffer solutions in different solvent systems to meet various experimental needs.