What are the main uses of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid
2-%28Tris%28Hydroxymethyl%29Methylamino%29Ethane - 1 - Sulphonic Acid, Chinese name 2 - (tris (methyl) methylamino) ethanesulfonic acid, TES. This is a commonly used method in biological molecular biology, and its main uses are as follows:
First, it can determine the pH of the solution. Biology is sensitive to the pH value of multiple biochemical reactions, and it may affect the activity of enzymes, proteins, and biomolecules. TES has good performance and can effectively control the pH value of the solution, so that it can be kept at a specific level, so as to protect the biochemical reaction. For example, in some enzymatic reactions, a specific pH is necessary for the activity of enzymes, and TES can be used to maintain this environment.
Second, it can be used for protein research. In the study of protein extraction, determination and function, it is necessary to determine the pH of proteins to prevent protein accumulation or aggregation. TES solution can provide a suitable environment to help proteins maintain their natural image activity, so that scientists can deeply explore their characteristics.
Third, nucleic acid research is also commonly used. Nucleic acid extraction, PCR amplification and swimming analysis, etc., all require specific pH components to ensure the efficiency of nucleic acid characterization and reaction. TES solution can be used to determine the pH value of both nucleic acid and nucleic acid-holding phase, making the results more reliable.
Therefore, 2 - (tris (methyl) methylamino) ethanesulfonic acid, by virtue of its pH-determining properties, plays a crucial role in the operation of biological chemistry, molecular biology, and related fields, providing a powerful tool for researchers to explore the secrets of biomolecules.
What are the advantages of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid in biological experiments
2 - (tris (hydroxymethyl) methylamino) ethane-1 -sulfonic acid, often called TES, has many advantages in biological experiments.
First, this substance has good buffering properties. Within a specific pH range, it can effectively resist the influence of adding a small amount of acid, base or moderate dilution, so that the pH of the system remains relatively stable. In many biochemical reactions, the activity of enzymes and the structure and function of proteins are extremely sensitive to pH. For example, when cells are cultured, the appropriate pH is the key to normal cell growth and metabolism. TES buffer can create and maintain such a suitable environment to ensure the stable development of cell experiments.
Second, the chemical properties of TES are stable. Under normal experimental conditions, it is not easy to chemically react with other substances in the system, and does not interfere with the experimental process and results. This is particularly important in complex biological system experiments, because it can ensure the stability of the buffer itself, and will not react with reactants, products or other reagents to generate interfering by-products, thus ensuring accurate and reliable experimental results.
Third, it has good compatibility with biomolecules. It has little effect on the structure and function of biological macromolecules such as proteins and nucleic acids, and will not cause denaturation or inactivation of biomolecules. In experiments such as protein purification and enzyme activity determination, it can provide a stable environment for biomolecules without changing their inherent characteristics and activities, which is helpful for obtaining true and accurate experimental data.
Fourth, good solubility. It is easily soluble in water and can conveniently configure buffers of different concentrations to meet various experimental needs. And the dissolution process is fast, which can quickly form a uniform and stable solution, which brings many conveniences to the experimental operation and improves the experimental efficiency.
What is the optimal concentration range for 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid?
2-%28Tris%28Hydroxymethyl%29Methylamino%29Ethane - 1 - Sulphonic Acid, Chinese name tris (methyl) methylaminoethanesulfonic acid, TES. The optimum degree of use varies depending on the needs and purposes.
In protein research, it is often used at a degree of 0.05 - 0.1M. Because of this degree of stability, it can effectively maintain the activity of the protein, and can reduce the acidity of the solution, so that it can be maintained at the appropriate pH of the protein.
If used in cell culture, the degree of resistance is usually 0.01 - 0.05M. This degree can not only provide the required normalization environment for cell production, but also do not cause adverse effects on cell transmission, etc., to ensure the normal production of cells.
However, in some enzymes, the optimum degree of resistance may fall within 0.1-0.2M. In this case, it can be used to effectively counteract the pH waves caused by factors such as substitution generation in the reaction process, so that the enzyme can develop high-efficiency catalytic activity at the most appropriate pH.
Therefore, the optimum degree of use of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1 - Sulphonic Acid can only be determined according to factors such as resistance and research.
What is the stability of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid?
2 - (tris (hydroxymethyl) methylamino) ethane-1 -sulfonic acid, often referred to as TES, has good stability.
This substance contains a special functional group structure and has a certain chemical stability. The sulfonic acid group (-SOH) is stable and can enhance the stability of the molecule in solution. The sulfonic acid group is a strong acidic group, which is not easy to be oxidized or reduced under common conditions, and the structure is stable.
From the perspective of the molecule as a whole, the tris (hydroxymethyl) methylamino part is an amino group connected to multiple hydroxymethyl groups. Hydroxymethyl has a certain electron donor effect, which can increase the density of the amino electron cloud, enhance the interaction with the sulfonic acid group, and improve the overall stability of the molecule.
TES is chemically stable and can maintain its own structural integrity in aqueous solution. In a wide pH range (about pH 6.0 - 8.5), it has good buffering performance and the structure is not easily damaged. And it is inert to most common chemical reagents. Under ordinary oxidation, reduction, acid-base reaction conditions, its molecular structure is stable. However, under extreme conditions such as high temperature and strong oxidants, the structure may be affected.
In addition, TES has good stability in solid state. If stored in a dry and cool environment, avoid light and moisture, it can maintain its own structure and properties for a long time, and is not prone to decomposition and deterioration.
Characteristics of 2- (Tris (Hydroxymethyl) Methylamino) Ethane-1-Sulphonic Acid Compared to Other Buffers
2 - (tris (hydroxymethyl) methylamino) ethane - 1 - sulfonic acid, often abbreviated as TES, has the following characteristics compared with other buffers:
First, it exhibits buffering advantages in a specific pH range. The effective buffering range of TES is about pH 6.8 - 8.2, which is suitable for many biochemical and molecular biology experiments. Compared with phosphate buffer, its buffer range is narrow but the pH is precisely regulated in the neutral alkali region. In enzymatic reactions such as enzymatic reactions that need to maintain the stability of this pH range, it can prevent the enzyme activity from being affected by pH fluctuations.
Second, the chemical stability is quite good. The molecular structure of TES makes it difficult to decompose or have side reactions with other substances under common experimental conditions. Like Tris buffer at high temperature or in the presence of some metal ions, chemical reactions may occur to affect the buffering effect, while TES has strong stability and can stably maintain pH in biochemical systems containing metal ions or experiments that require high temperature treatment of samples.
Third, it has little interference with biological systems. In biological experiments such as cell culture and protein purification, many buffers may interact with biomolecules and interfere with experimental results. TES charge distribution and molecular size make it weakly interact with biological macromolecules such as proteins and nucleic acids, and do not interfere with the activity and structure of biomolecules, which is conducive to obtaining accurate experimental data.
Fourth, good solubility. Whether it is in water or common organic solvents, TES can be well dissolved, which is convenient for the experimental configuration of buffer solutions in different solvent systems to meet various experimental needs.