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What is the use of (2R) -2-Hydroxy-3- {[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Ammonio} Propane-1-Sulfonate
(2R) -2-hydroxy-3- {[2-hydroxy-1,1-bis (hydroxymethyl) ethyl] ammonium} propane-1-sulfonate is widely used in biochemical research. It is mild in nature and has little interference with the biochemical system, so it is often used by researchers to maintain the acid-base balance of the system.
In human organisms, many biochemical reactions depend on a suitable acid-base environment. Due to its good buffering properties, this substance can effectively resist the impact of external acid-base changes, making the pH value of the reaction system as stable as Mount Tai. For example, in the research of proteins and enzymes, the activities of proteins and enzymes are highly susceptible to pH fluctuations, and their conformation and function will be damaged if they are not careful. And (2R) -2-Hydroxy-3- {[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Ammonium} Propane-1-Sulfonate can skillfully adjust and maintain the pH of the system within a specific pH range, maintaining the activity of proteins and enzymes, so that the experiment can proceed smoothly.
Furthermore, in the field of cell culture, the growth and metabolism of cells also have strict requirements on the pH of the environment. Adding this substance to the cell culture fluid can create a stable acid-base environment, create a comfortable growth home for cells, and assist in the normal proliferation and differentiation of cells. Therefore, it is also an indispensable reagent in the research and practice of cell biology.
What are the chemical properties of (2R) -2-Hydroxy-3- {[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Ammonio} Propane-1-Sulfonate
(2R) - 2-hydroxy-3- {[2-hydroxy-1,1-bis (hydroxymethyl) ethyl] ammonium} propane-1-sulfonate This substance has many unique chemical properties.
In terms of solubility, it has good solubility in water. Because its molecules contain multiple hydroxyl groups, ammonium groups, and sulfonic acid groups, these groups are hydrophilic and can form hydrogen bonds with water molecules, which promotes the substance to be easily soluble in water and form a homogeneous dispersion solution.
In terms of stability, the structure is quite stable at room temperature, pressure, and general environment. In case of strong acid and alkali, its molecular structure may change. Because the hydroxyl group, ammonium group and sulfonic acid group in the molecule are sensitive to acid and alkali, under the condition of strong acid and alkali, these groups may participate in the reaction and cause structural changes.
In terms of acidity and alkalinity, the sulfonic acid group can ionize hydrogen ions in water, making the solution acidic. However, the hydroxyl group and ammonium group in the molecule have certain alkalinity and can react with acid. Therefore, the overall acidity and alkalinity are restricted by the solution environment and the interaction of various groups.
In terms of redox properties, although there are no typical strong oxidation or strong reduction groups in the molecule, the hydroxyl group can be oxidized under specific conditions if it encounters a strong oxidizing agent.
This substance can participate in many chemical reactions, such as esterification and substitution, because it contains a variety of active groups, and has potential application value in the field of organic synthesis.
What is the market price of (2R) -2-Hydroxy-3- {[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Ammonio} Propane-1-Sulfonate
Today there are (2R) - 2 -hydroxy-3- {[2 -hydroxy-1,1 -bis (hydroxymethyl) ethyl] ammonium} propane-1 -sulfonate, what is the market price? This is a fine chemical with a wide range of uses and is used in biochemical research, pharmaceutical preparations and other fields. Its price varies depending on quality, purity, manufacturer, and purchase quantity.
If you look for various cities, common reagent-grade products with higher purity may cost tens of yuan per gram. If purchased in large quantities, in kilograms, the unit price may be reduced to tens of yuan per kilogram. However, this is not fixed, the market conditions are changeable, and different suppliers have different prices.
If there are manufacturers customizing high-purity special specifications, the price is higher, or due to customization costs, R & D investment and other factors, or more than 100 yuan per gram. To know the exact price, you need to consult the reagent supplier, chemical product distributor, and carefully check the quotation to obtain the accurate number.
What are the synthesis methods of (2R) -2-Hydroxy-3- {[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Ammonio} Propane-1-Sulfonate
The synthesis method of (2R) -2-hydroxy-3- {[2-hydroxy-1,1-bis (hydroxymethyl) ethyl] ammonium} propane-1-sulfonate is described in detail below.
To synthesize this compound, the common method is to obtain it from a starting material with a specific structure through a multi-step reaction. First, a compound containing suitable functional groups, such as hydroxyl groups and sulfonic acid groups, is selected and reacted with a reagent that can introduce ammonium groups.
At the beginning of the reaction, the compound with hydroxyl group and the reagent containing amine group with suitable substituent group can undergo nucleophilic substitution reaction under appropriate reaction conditions, such as suitable temperature, pH and catalyst. This reaction aims to introduce ammonium groups to specific positions of the target molecule.
Next, appropriate protection and deprotection operations are required for the sulfonic acid group to ensure the selectivity and yield of the reaction. When protecting the sulfonic acid group, a specific protective group can be selected. After the subsequent reaction is completed, it can be removed under mild reaction conditions to restore the structure of the sulfonic acid group.
For the hydroxyl moiety, caution is also required. The hydroxyl group can be protected in a timely manner according to the reaction process to prevent it from participating in unnecessary reactions and interfering with the main reaction process. After the construction of key parts such as ammonium group and sulfonic acid group is completed, the hydroxyl group is modified or deprotected accordingly to obtain the final target product.
The whole synthesis process requires precise control of the conditions of each step of the reaction, and accurate separation and identification of the reaction intermediates, so that (2R) - 2-hydroxy-3 - {[2-hydroxy-1,1-bis (hydroxymethyl) ethyl] ammonium} propane-1-sulfonate can be successfully synthesized with high yield and purity.
What are the storage conditions for (2R) -2-Hydroxy-3- {[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Ammonio} Propane-1-Sulfonate
(2R) - 2-hydroxy-3- {[2-hydroxy-1,1-bis (hydroxymethyl) ethyl] ammonium} propane-1-sulfonate, which is a rather special chemical substance. Its storage conditions are extremely critical to the stability of its chemical properties and the maintenance of its utility.
According to the simple wisdom contained in "Tiangong Kaiwu", all substances need to be adapted to their unique storage methods. This (2R) -2-hydroxy-3- {[2-hydroxy-1,1-bis (hydroxymethyl) ethyl] ammonium} propane-1-sulfonate should be placed in a cool and dry place. A cool place can avoid the chemical structure variation caused by high temperature, just like the ancients hid things in a secluded room to prevent the disturbance of heat. Dry places can avoid moisture erosion, because moisture often causes substances to deliquescence and deterioration, just like ancient books afraid of moisture, they need to be hidden in a dry place.
And it needs to be isolated from the air, because some of its components may easily react with oxygen and other components in the air, just like ancient medicinal pills, sealed and stored to prevent it from interacting with the outside air and changing. When stored, it should also be kept away from fire sources and strong oxidants. Fire sources can easily cause it to burn and explode, and strong oxidants come into contact with it, or cause violent chemical reactions, causing it to lose its original properties, just like ancient gunpowder is kept away from fire and strong objects. In this way, it can be properly preserved and its quality and effectiveness can be guaranteed.