3-[4-(2-Hydroxyethyl)-1-Piperazine]Propanesulfonic Acid

3 - [4- (2-hydroxyethyl) -1 -piperazine] propanesulfonic acid, its chemical structure is as follows:
The core of this compound is a piperazine ring, which is connected to a 2-hydroxyethyl group at the 4-position of the piperazine ring, and a propanesulfonic acid group at the 1-position. Structurally, the piperazine ring is a six-membered nitrogen-containing heterocycle with two nitrogen atoms in the para-position. In the 2-hydroxyethyl group, the hydroxyl group is connected to the ethyl group at the end, and the ethyl group is connected to the 4-position nitrogen atom of the piperazine ring, giving the structure a certain hydrophilicity. The hydroxyl group can participate The 1-linked propanesulfonic acid group is composed of propyl group and sulfonic acid group, and the sulfonic acid group-SO-H is a strong acidic group, which makes the compound have good water solubility and ionic characteristics, and plays an important role in biochemical research and buffer system construction. The overall structure makes it both hydrophilic and ionic, and has unique application value in many fields.

3 - [4- (2-hydroxyethyl) -1 -piperazine] propanesulfonic acid, which is a commonly used biological buffer. Its physical properties are unique and worth exploring.
Looking at its properties, it is mostly in the state of white crystalline powder under normal conditions, with a fine texture, like the first snow in winter, pure and uniform. This form is easy to weigh and use, and it is very convenient for experimental operation.
When it comes to solubility, it exhibits good solubility in water. Just like a fish entering water, it quickly fuses with water to form a uniform and stable solution. This property makes it possible to quickly reach the required concentration when preparing the buffer, laying the foundation for the efficient development of the experiment. And its aqueous solution is clear and transparent, almost colorless, like pure lake water, without impurity interference, providing a clear medium for experimental observation and detection.
In addition to the melting point, its melting point is within a specific range, which is of great significance for its stability and purity. The precise melting point value is similar to the label of product quality, ensuring the reliability and stability of its performance in various application scenarios.
The density of this substance is also an important physical property. The appropriate density allows it to be integrated into the system in an appropriate proportion when participating in chemical reactions or solution preparation to maintain the overall balance and stability.
In addition, its hygroscopicity cannot be underestimated. In the air, it absorbs water like a sponge, and has a tendency to absorb water. Therefore, it needs to be properly sealed during storage to prevent moisture deterioration and ensure that its properties are not disturbed by moisture and remain consistent.
3- [4- (2-hydroxyethyl) -1 -piperazine] propanesulfonic acid plays an indispensable role in many experimental fields such as biochemistry and molecular biology due to these physical properties, providing a strong guarantee for the smooth progress of scientific research.

3- [4- (2-hydroxyethyl) -1-piperazine] propanesulfonic acid, often referred to as HEPPS, is widely used in biochemical research, drug research and development, food and beverage, agricultural science and other fields.
In the field of biochemical research, this is a commonly used biological buffer. Because it can effectively maintain the stability of the pH value of the system and create a suitable environment for biochemical reactions, many enzymatic reactions and protein crystallization experiments rely on it to stabilize the pH to ensure accurate and reliable experimental results.
In the process of drug development, this substance has a significant effect. In the process of drug preparation, it can adjust the pH value of drug solution, improve drug stability and solubility, and ensure drug quality and efficacy. For example, for some injections, HEPPS is required to maintain a suitable pH to avoid drug deterioration or inactivation.
In the food and beverage industry, it can be used as an acidity regulator. It can regulate the pH of food and beverages, improve taste, and prolong shelf life. Like some fruit juice drinks, adding HEPPS can stabilize pH, prevent microbial growth, and optimize flavor.
In the field of agricultural science, when plant cells are cultured, HEPPS can adjust the pH of the medium and promote plant cell growth and differentiation. In soil improvement research, it can also be used to adjust soil pH to create a good soil environment for plant growth.
In summary, 3- [4- (2-hydroxyethyl) -1-piperazine] propanesulfonic acid plays a key role in many fields and has made outstanding contributions to the development of various fields.

3- [4- (2-hydroxyethyl) -1 -piperazine] propanesulfonic acid, often referred to as HEPPS, is a commonly used zwitterion buffer in biochemical research. Many matters need to be paid attention to when using it, as detailed below:
First, the pH range is considered. The buffer range of HEPPS is about pH 7.8-9.2. When using it, it is necessary to ensure that the pH value of the system you want to maintain falls within this range in order to exert its good buffering efficiency. If the pH of the system deviates too much from this range, the buffering capacity of HEPPS will be greatly reduced, and it is difficult to effectively maintain the stability of the pH of the system.
Second, the precise control of the concentration. The concentration of buffering agent has a great influence on its buffering capacity. If the concentration is too low, the buffering capacity will be insufficient in the face of acid-base changes; if the concentration is too high, it may interfere with the experimental system, such as affecting protein activity. Therefore, before the experiment, the appropriate HEPPS concentration should be determined by calculation or pre-experiment according to specific needs.
Third, solubility issues. Although HEPPS has a certain solubility in water, the dissolution process may be affected by factors such as temperature and water quality. In order to ensure its full dissolution, it can be properly heated and stirred. If the water quality is poor and contains impurities, it may also affect its dissolution effect. At the same time, after dissolution, it is necessary to pay attention to whether the solution is clear, whether there are insolubles, and if so, it may need to be filtered.
Fourth, compatibility with other substances. When using HEPPS in complex biochemical systems, it is necessary to pay attention to its interaction with other components in the system, such as proteins, enzymes, metal ions, etc. In some cases, HEPPS may chemically react with these substances, changing their chemical properties or biological activities, and then interfering with the experimental results. Before the experiment, the components in the system should be fully understood, and compatibility pre-experiment should be carried out if necessary.
Fifth, the importance of storage conditions. HEPPS should be stored in a dry, cool and well-ventilated place to avoid moisture, heat and direct sunlight. Moisture can easily cause it to agglomerate and affect the use; too high temperature or too much light, or cause changes in its chemical structure, reducing buffer performance. It is also necessary to pay attention to the shelf life of expired products. If their performance has changed, their quality should be checked before use.

To prepare 3- [4- (2-hydroxyethyl) -1 -piperazine] propanesulfonic acid, the following method can be followed.
First take an appropriate amount of 1-piperazine ethanol and place it in a suitable reaction vessel. In addition, prepare propylation reagents containing sulfonic acid groups, such as 3-halopropanesulfonic acid or its corresponding active derivatives. Under suitable reaction conditions, such as in an organic solvent, add an appropriate amount of base to promote the reaction. The base can be selected from common inorganic bases such as potassium carbonate and sodium carbonate, which can promote the smooth progress of nucleophilic substitution reactions.
When reacting, the temperature and reaction time need to be controlled. The temperature should not be too high or too low, generally under moderate heating conditions, such as between 40 and 80 degrees Celsius, depending on the activity of the specific reactants. Continuous stirring allows the reactants to fully contact and accelerate the reaction process. The reaction time depends on the monitoring of the reaction process. The reaction can be tracked by thin layer chromatography (TLC) and other means until the raw material point is basically eliminated, that is, the reaction tends to be complete.
After the reaction is completed, the reaction mixture is post-processed. First extract with a suitable solvent to separate the organic phase. Then wash and dry the organic phase to remove impurities. Subsequently, the organic solvent is removed by vacuum distillation and other methods to obtain a crude product.
For further purification of the crude product, column chromatography can be selected. With a suitable eluent system, the separation of the product and the impurity is achieved according to the difference in the partition coefficient between the fixed phase and the mobile phase, and the final product is pure 3- [4- (2-hydroxyethyl) -1 -piperazine] propanesulfonic acid. In this way, the desired product can be obtained through multi-step operation.