3-(4-Morpholine)Propanesulfonic Acid, Sodium Salt

Sodium 3- (4-morpholinyl) propane sulfonate is a useful chemical substance. It has excellent buffering properties and is widely used in biochemical and molecular biology experiments.
This substance is stable in properties, not easy to decompose under common experimental conditions, and can effectively maintain the pH stability of the system. Its buffer range is suitable, usually between pH 6.5 and 7.9, which can meet the strict requirements of many biochemical reactions for specific pH.
has good solubility in water, and can quickly dissolve with water to form a uniform solution, which provides convenience for experimental operation. And it has good compatibility with most biomolecules, rarely interacts with biological macromolecules such as proteins and nucleic acids, and does not affect their structure and function. Therefore, it is widely used in protein purification, enzyme activity determination, cell culture and many other experimental scenarios.
Because of its stable chemical properties and specific functions, it is an indispensable and important reagent in the field of life science research, helping researchers accurately simulate the environment in organisms and promoting scientific research.

3 - (4 - morpholinyl) propanesulfonate sodium salt, which has a wide range of uses, is often used as a buffer in the field of biochemical research. It can maintain the stability of the pH of the reaction system and make the biochemical reaction proceed smoothly. In the research experiments of proteins and nucleic acids, the stability of the buffer environment is essential to ensure the accuracy of the experimental results. This salt can create appropriate acid-base conditions to avoid changes in the structure and function of proteins and nucleic acids due to pH fluctuations.
In the process of cell culture, 3 - (4 - morpholinyl) propanesulfonate sodium salt is also indispensable. Cell growth is extremely sensitive to the pH of the environment, and a slight deviation will affect the proliferation, differentiation and even survival of cells. The salts can effectively regulate the pH value of the medium, create a stable and suitable growth environment for cells, and ensure normal physiological activities of cells.
Furthermore, in the experimental stage of drug development, the stability and activity of drugs are often related to the pH of the environment. Sodium 3 - (4 - morpholino) propane sulfonate can be used to simulate the pH of the physiological environment, helping researchers to explore the behavior of drugs in vivo, such as drug solubility, stability, and interaction with targets, providing key data support for drug development.

3 - (4 - morpholinyl) propanesulfonate sodium salt, this is a commonly used reagent for biochemical experiments, and many points need to be paid attention to when storing.
Bear the brunt, temperature control is essential. It should be stored in a low temperature environment, usually 2-8 ° C. Due to high temperature, it is easy to cause chemical changes in the reagent, or cause its structure to be damaged and its activity to be reduced, which in turn affects the accuracy of the experimental results. For example, if food is not properly refrigerated on a hot summer day, it is very perishable and deteriorated.
Furthermore, humidity cannot be ignored. It needs to be stored in a dry place to prevent moisture. Because of its certain water absorption, if the ambient humidity is high, it will absorb water, causing the concentration of the reagent to change, and some components may undergo reactions such as hydrolysis due to the action of water, which will damage the quality of the reagent. Just like wood tools are prone to mildew and decay in a humid environment, the reagent will also "deteriorate" when damp.
It is also key to store in the dark. The reagent may be sensitive to light, and it may cause luminescent chemical reactions under light to change its chemical properties. Therefore, it should be placed in a brown bottle or other dark container and stored in a dark place to avoid direct light, just like precious calligraphy and paintings need to be stored in the dark to prevent fading.
In addition, when storing, keep away from strong oxidizing agents, strong acids and alkalis and other Due to their chemical properties or violent reactions with these substances, the reagents become ineffective and even dangerous. This is like water and fire are incompatible, and the two types of substances with very different properties are close to the trouble.
During storage, the status of the reagents should also be checked regularly to see if there are any abnormalities such as discoloration and precipitation. If there is any abnormality, it needs to be dealt with in time to avoid affecting subsequent use. In this way, it can ensure that the properties of 3 - (4 - morpholino) propane sulfonate sodium salt are stable during storage and play its due role in the experiment.

3- (4-morpholinyl) propanesulfonate sodium salt, the preparation method, although the ancient books did not directly describe this medicine, but looking at the principles of chemistry and pharmaceutical methods, we can find a way.
First, take morpholine and sodium propenesulfonate as the starting materials. First, mix the morpholine with a suitable solvent, and slowly add sodium propenesulfonate at the appropriate temperature. Stir to make the two blend and promote the reaction. Both temperature and stirring rate are required. If the temperature is too high, the reaction will be too fast, and it is easy to produce impurities; if the temperature is too low, the reaction will be slow and take a long time. Stir well to make the raw materials fully contact and the reaction will be complete.
Second, or use 3- (4-morpholino) propanesulfonic acid to react with sodium hydroxide. Dissolve 3- (4-morpholino) propanesulfonic acid in a solvent and gradually add sodium hydroxide solution. This process requires the agent indicated by acid and base to observe the reaction progress. When the appropriate pH value is reached, the reaction is completed. After separation and purification, a pure 3- (4-morpholino) propanesulfonic acid sodium salt is obtained.
When separating, evaporation and crystallization can be used. The reaction solution evaporates the solvent at a specific temperature, and the solute gradually resolves into crystals. Then it is filtered and washed to remove its impurities. At the time of purification, or by recrystallization, it is dissolved in a suitable solvent and then slowly crystallized to obtain a pure product.
Pharmaceutical work requires caution and compliance with procedures at all steps to obtain high-quality 3- (4-morpholinyl) propane sulfonate sodium salt.

Sodium 3- (4-Morpholine) Propanesulfonic Acid (Sodium Salt), often used as a biological buffer, maintains the pH stability of the system in biochemical experiments. Its reaction with other reagents varies depending on the nature of the reagent, as follows:
1. ** Reacts with acid **: This salt is weakly basic. In case of strong acid, the morpholine ring nitrogen atom will bind to the proton to form the corresponding conjugated acid, and at the same time form a sodium salt. In case of hydrochloric acid, the reaction formula is:\ [C_7H_ {14} NNaO_4S + HCl\ longrightarrow C_7H_ {15} NO_4S + NaCl\] The product is 3- (4-morpholino) propanesulfonic acid and sodium chloride. The amount of strong acid is large, and the pH of the solution is significantly reduced.
2. ** Reaction with base **: Because of the acidic nature of the sulfonic acid group, it can react with strong bases. For example, when reacted with sodium hydroxide, the sulfonic acid hydrogen is replaced by sodium to form water and another sodium salt. The reaction formula:\ [C_7H_ {14} NNaO_4S + NaOH\ longrightarrow C_7H_ {13} NNa_2O_4S + H_2O\] The amount of strong base is large, and the pH of the solution increases.
3. ** Reaction with metal ions **: Sulfonic acid can complex with some metal ions. If it reacts with calcium ions, an insoluble precipitate or complex is formed, which affects the properties of the solution and the activity of metal ions. The reaction formula:\ [2C_7H_ {14} NNaO_4S + Ca ^ {2 +}\ longrightarrow (C_7H_ {14} NO_4S) _2Ca + 2Na ^ +\]
4. ** Reacts with an oxidizing agent **: The morpholine ring and the sulfonic acid group may be oxidized under the action of a strong oxidizing agent. In the case of hydrogen peroxide, the morpholine ring may open the ring or oxidize the sulfonic acid group to a higher valence sulfur compound, changing the molecular structure and properties.
5. ** Reacts with a reducing agent **: Under certain conditions, certain groups of the compound can be reduced. For example, under the action of a strong reducing agent, the morpholine ring may be hydrogenated and reduced, altering its chemical activity and molecular configuration.