3-(4-Morpholino)Propane Sulfonic Acid，Sodium Salt

Sodium 3- (4-morpholinyl) propanesulfonate is a commonly used reagent in biochemical research and other fields. Its chemical properties are unique, let me tell them one by one.
First of all, it has good water solubility. This property makes it easy to disperse and dissolve in aqueous solutions, and can easily participate in various chemical reactions or biochemical experimental processes. Because it can be evenly distributed in water, it is conducive to interaction with other water-soluble substances.
Secondly, the compound has certain buffering properties. Within a specific pH range, it can play a buffering effect on the pH of the solution and maintain the relative stability of the pH value of the solution. This point is crucial in many pH-sensitive biochemical reactions or experimental systems. It can create a suitable and stable acid-base environment for the reaction, ensure the smooth progress of the reaction, and avoid adverse effects on the reaction results due to large fluctuations in pH.
Furthermore, the morpholine group and sulfonic acid group contained in its structure give it special chemical activity. Morpholine group has a certain alkalinity and can participate in some acid-base neutralization or nucleophilic reactions; sulfonic acid group has strong hydrophilicity and acidity, can enhance the water solubility of the compound, and can also be used as an activity check point in some reactions, substitution, condensation and other reactions with other substances.
In addition, the stability of this substance is relatively high. Under normal storage and use conditions, it generally does not decompose or deteriorate easily, making it easy to store and use for a long time. However, care should be taken to avoid contact with strong oxidizing agents, strong acids, strong bases and other highly corrosive or reactive substances to prevent unexpected chemical reactions and cause changes in its chemical properties.
In summary, sodium 3- (4-morpholino) propanesulfonate has shown important application value in many fields such as biochemistry and medicine due to its unique chemical properties.

Sodium 3- (4 -morpholinyl) propanesulfonate has many applications in the fields of biochemistry and molecular biology. The construction of a buffer system is crucial for the complicated biochemical experiments of Guanfu. This salt can be used as a buffer to maintain the pH of the system in a stable state in many biochemical reactions. Because the structure and function of biological macromolecules, such as proteins and nucleic acids, are extremely sensitive to the pH of the environment, slight changes may cause their activity to be damaged or even inactivated.
Furthermore, in molecular biology experiments, such as polymerase chain reaction (PCR), gel electrophoresis and other common operations, a stable acid-base environment is indispensable. The buffer system constructed by 3- (4-morpholinyl) propanesulfonate sodium salt can provide suitable acid-base conditions for various enzymatic reactions to ensure that the reactions proceed smoothly in sequence.
In the field of cell culture, cell growth and metabolism also have strict requirements on the pH of the environment in which they are located. This salt can be used to prepare cell culture medium to create an excellent microenvironment for cell growth, so that cells can proliferate and differentiate normally in a stable acid-base atmosphere, and avoid the harm of acid-base imbalance.

The method for preparing the sodium salt of 3- (4-morpholinyl) propanesulfonate is described below.
First, prepare the raw materials. 4-morpholinesulfonate (MES) and sodium hydroxide (NaOH) are required. Both are common chemicals and are available from most chemical reagent suppliers. MES is the key starting material, and its structure contains morpholine groups to provide the necessary part of the target product; NaOH is used in the neutralization reaction to form a sodium salt.
Next, perform the reaction step. In a suitable reaction vessel, dissolve MES in an appropriate amount of deionized water, stir until completely dissolved, and obtain a homogeneous solution. In this step, it is necessary to pay attention to the fact that the water temperature should not be too high or too low, and room temperature is better to ensure that the MES dissolves well and does not affect its chemical properties. Then, slowly add the pre-prepared NaOH solution dropwise to the MES solution, and continue to stir. This process requires strict control of the dropwise rate of NaOH to prevent the reaction from being too violent. During the reaction, the sulfonic acid group of MES neutralizes with NaOH to generate 3- (4-morpholino) sodium propane sulfonate.
Furthermore, reaction monitoring. The pH of the reaction system can be monitored with the help of pH test paper or pH meter. When the pH value of the solution reaches a specific range (about 7-8, fine-tuned according to the specific reaction conditions), it indicates that the reaction is close to completion. At this time, the MES in the reaction system is basically converted into the target product 3- (4-morpholino) propanesulfonate sodium salt.
Finally, the product is separated and purified. After the reaction is completed, the evaporation crystallization method is used. The reaction solution is placed in an evaporation dish, and the solvent water is slowly evaporated under the heating condition of a water bath. As the water evaporates, the 3- (4-morpholino) propanesulfonate sodium salt gradually crystallizes and precipitates. When most of the solvent evaporates and more crystals appear, stop heating and cool naturally to make the crystals further precipitate completely. Subsequently, the Brinell funnel and suction filtration device are used for suction filtration to separate the crystals from the mother liquor. Then the crystals are washed several times with an appropriate amount of cold deionized water to remove impurities and Finally, the crystals were placed in an oven and dried at a suitable temperature (about 50-60 ° C) to obtain a pure 3- (4-morpholino) propane sulfonate sodium salt product.

Sodium 3- (4-morpholinyl) propanesulfonate, when storing and using, many things need to be paid attention to. This is a chemical reagent with special properties, which is related to experimental accuracy and safety, and should not be underestimated.
In terms of storage, the first priority is dry environment. Moisture is easy to cause deliquescence and affect quality, so it should be stored in a dry and ventilated place, away from water sources and humid corners. Secondly, temperature is also critical, and it should be placed in a cool environment to avoid high temperature. High temperature may deteriorate the ingredients and reduce the efficacy. Furthermore, it needs to be sealed and stored to prevent excessive contact with air to avoid oxidation and other reactions.
When using, the operating specifications are essential. Before use, make sure that the utensils used are clean and dry to prevent impurities from mixing in. When weighing, make sure to be accurate, and strictly weigh according to the amount required by the experiment. Too much or too little may affect the results. When dissolving, proceed with the solvent according to the specified method, and the stirring speed and time should also be moderate to ensure full dissolution. At the same time, the experimenter must be protected and wear suitable protective equipment, such as gloves, goggles, etc., because the substance may be irritating to the human body. In addition, the use process should pay attention to the reaction conditions, strictly control the temperature, pH and other factors to ensure the smooth progress of the experiment and obtain accurate results. In this way, 3 - (4 - morpholinyl) propane sulfonate sodium salt can be properly stored and used.

Sodium 3- (4-morpholinyl) propanesulfonate is an important biochemical reagent, and its Quality Standards are more elegant.
First, the appearance must be excellent. It should be a white crystalline powder with pure color and no variegated or foreign matter. This is the basic sign. If the appearance is not good, or contains impurities, it will affect the follow-up experiment.
Second, purity is very important. Purity often needs to be ≥ 99.0%, high purity can ensure the accuracy and reliability of the experimental results. Impurities can interfere with the reaction and cause experimental deviations. It can be measured by high performance liquid chromatography (HPLC), mass spectrometry (MS) and other methods.
Third, pH value is strict. At 25 ℃, the pH value of 1% aqueous solution is about 7.0 - 9.0. Improper pH affects the buffer system and interferes with the activity and reaction process of biomolecules.
Fourth, the drying weight loss is normal. Usually ≤ 0.5%, there is a lot of weight loss, or there is too much water, which affects the stability and concentration accuracy of the reagent. It can be measured by oven drying method.
Fifth, the content of heavy metals is strictly controlled. The total amount of heavy metals such as lead and mercury is ≤ 10ppm. Heavy metals can poison the biological system and cause experimental illusions or wrong conclusions.
Sixth, the water solubility is better. It is easily soluble in water and reaches a uniform solution quickly. If the solubility is poor, it will affect the dispersion and reaction efficiency of the reagent.