3-[Bis(2-Hydroxyethyl)Amino]-2-Hydroxypropanesulphonic Acid

3- [Bis (2-hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, often referred to as DIPSO, is a key zwitterion buffer in biochemical research. It has a wide range of uses and plays an important role in many fields.
In biochemical and molecular biology experiments, this buffer has a wide range of uses. First, it can effectively maintain the pH stability of the reaction system. Many biochemical reactions are extremely sensitive to pH value, and even a slight change in pH value may have a significant impact on the reaction process and enzyme activity. With its specific acid-base balance characteristics, DIPSO can stabilize the pH value of the reaction system within a specific range, creating excellent conditions for the smooth progress of biochemical reactions. For example, in enzymatic reaction experiments, by adding an appropriate amount of DIPSO buffer, the enzyme can be ensured to be in the optimum pH environment, so as to give full play to its catalytic activity and make the reaction proceed efficiently.
Second, in the field of protein and nucleic acid research, DIPSO is also indispensable. The structure and function of proteins and nucleic acids are highly susceptible to environmental factors, and pH is a key one. DIPSO can create a stable pH environment, which helps to maintain the natural structure and function of proteins and nucleic acids, and avoid their denaturation or inactivation due to pH fluctuations. In protein crystallization experiments, a suitable DIPSO buffer system helps proteins form regular crystal structures, which lays the foundation for subsequent analysis of protein three-dimensional structures by X-ray crystallography.
Third, DIPSO is also often used in the field of cell culture. Cells have strict pH requirements for the growth environment, and a suitable pH environment is the basis for normal cell growth, metabolism and proliferation. DIPSO buffers can effectively regulate the pH value of cell culture medium, providing a stable living environment for cells and facilitating the smooth development of cell culture experiments.
In addition, DIPSO is also used in clinical diagnostic reagents. Some diagnostic methods need to be carried out under specific pH conditions, and DIPSO can be added to the diagnostic reagent as a buffer component to ensure that the detection reaction occurs in a suitable pH environment, thereby improving the accuracy and reliability of the detection results.

3- [Bis (2-hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, often referred to as DIPSO, is a commonly used zwitterion buffer in biochemical research. Its physical properties are worth exploring.
Looking at its properties, under normal temperature and pressure, this substance is a white crystalline powder with a clean appearance, fine texture, and no impurities visible to the naked eye. This property is crucial in experimental operation, because its pure state can ensure the accuracy of experimental results.
In terms of solubility, DIPSO is highly soluble in water, and can be quickly and evenly dispersed in water to form a clear and transparent solution. This good water solubility makes it convenient to prepare a buffer at the required concentration in various biochemical experiments. However, it has little solubility in common organic solvents such as ethanol and ether. This difference in solubility helps to select the appropriate solvent for separation, purification or reaction according to the needs during the experimental process.
Melting point is also an important physical property. The melting point of DIPSO is quite high, about 225-228 ° C. A higher melting point means that its chemical structure is relatively stable. It can maintain solid state stability within the temperature fluctuation range of the general experimental environment, and is not easy to change the morphology due to temperature changes, thus ensuring the stability of the experimental system.
The density of DIPSO is about 1.399g/cm ³. This density data is of great significance for accurately weighing and configuring a solution of a specific concentration. In the experimental operation, knowing its density accurately can accurately calculate the mass of the required DIPSO according to the required solution volume and concentration, thereby ensuring the consistency and accuracy of the experimental conditions.
In addition, its aqueous solution exhibits good buffering properties, which can effectively maintain the pH stability of the solution within a certain pH range. Generally speaking, the buffer range of DIPSO is about pH 6.9 - 8.3. This buffering property is crucial in many biochemical reactions. The structure and function of many biomacromolecules such as proteins and nucleic acids are extremely sensitive to the pH of the environment in which they are located. With its stable buffering performance, DIPSO creates a suitable and stable acid-base environment for these biochemical reactions, which helps to carry out the experiment smoothly and ensure the scientific and reliable experimental results.

3- [Bis (2-hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, which is a white crystalline powder, is often used as a buffer in the field of biochemical research.
It has good buffering properties and can effectively maintain the pH stability of the solution within a specific pH range. The water solubility of this substance is quite good, and it can be easily dissolved in water to form a uniform solution. This property makes it easier to apply in experimental operations.
Its chemical stability is also excellent. Under conventional storage conditions, its properties are relatively stable, and it is not easy to decompose or deteriorate. It can be stored for a long time without losing its effectiveness.
Furthermore, 3- [bis (2-hydroxyethyl) amino] -2-hydroxypropanesulfonic acid has little effect on biological systems and does not cause significant interference with the structure and function of biological macromolecules. Therefore, it is widely used in many experiments in biochemistry and molecular biology to ensure the suitability of the experimental environment and lay the foundation for the accuracy and reliability of the experimental results.

3 - [Bis (2 - Hydroxyethyl) Amino] - 2 - Hydroxypropanesulphonic Acid, Chinese name, diethanolamine-propyl sulfonic acid, often used in DIPSO, is commonly used in biological and chemical research. To save this material, you need to pay attention to the following general things:
First environmental protection. It should be placed in the environment and dry, avoid direct light exposure. Because light can induce actinic reaction of the material, and affect its transformation and characterization. If exposed to light, DIPSO molecules are damaged or damaged, and their performance can be changed to maintain the pH environment in the environment.
Furthermore, pay attention to degree control. The high temperature can accelerate the molecular temperature, promote the acceleration of the reaction rate, and may cause decomposition or degradation; the low temperature, or cause its crystals to precipitate, and use the same color. Therefore, it is generally recommended to store in the room (15-30 ° C), and it can be stored according to the requirements of the product specification.
Sealed storage is also important. DIPSO is easy to interact with certain components in the air, such as water evaporation, carbon dioxide, etc. Once moisture is absorbed, or its temperature is changed, the effect will be affected; if carbon dioxide is reversed, carbonate and other substances may be formed, which will break the original resistance. Therefore, after use, the container must be sealed.
In addition, it is necessary to store oxidized, original and other incompatible materials. DIPSO chemical properties or part of the product can be reversed. If it is oxidized, it may oxidize DIPSO and improve its molecular properties. Therefore, in the storage, it is necessary to ensure that the product is not in the same room or near the same location.
The most important, check its external appearance and valid period regularly. If there is a color, taste or valid period, even if it has not been used, it cannot be reused, so as not to affect the effectiveness of the fruit.

To prepare 3- [bis (2-hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, the method is as follows:
First, prepare the required materials, such as compounds containing sulfonic acid groups, reactants with bis (2-hydroxyethyl) amino groups, and appropriate solvents, catalysts, etc. Among them, the reactants containing sulfonic acid groups and the reactants with bis (2-hydroxyethyl) amino groups should be selected with high purity to ensure the quality of the products.
times, in a clean and dry reaction vessel, add the materials in a certain proportion. Usually, the molar ratio of the reactants is controlled to achieve the best reaction effect. For example, compounds containing sulfonic acid groups and reactants with bis (2-hydroxyethyl) amino groups can be mixed according to a specific molar ratio, and according to the characteristics of the reaction, an appropriate amount of solvent should be added to make the material fully dispersed to facilitate the progress of the reaction.
Next, adjust the conditions of the reaction. It is often necessary to control the temperature, pressure and time of the reaction. The temperature depends on the reaction mechanism and the activity of the selected reactants, or gradually rises or falls within a specific temperature range to promote the reaction to produce the target product. Pressure is also the key, or normal pressure, or a certain positive or negative pressure is applied, depending on the needs of the reaction. The control of time is related to the yield and purity of the product, and the reaction time needs to be monitored according to the experiment.
During the reaction, it can be stirred in a timely manner to make the material fully contact and the reaction is uniform. And analytical means, such as chromatography, are often used to monitor the progress of the reaction to understand the degree of reaction and the formation of the product.
After the reaction is completed, the steps of post-treatment are performed. Or first separated, such as filtration, centrifugation, etc., to remove unreacted solid impurities or catalysts. After purification, the purity of the product is extracted by distillation, crystallization, extraction, etc. For example, by crystallization, the cooling rate of the solution and the crystallization temperature are controlled, so that the product can be precipitated with high purity crystals.
In this way, through various steps, 3- [bis (2-hydroxyethyl) amino] -2-hydroxypropanesulfonic acid can be obtained.