Products

3 - (Cyclohexy-Lamine) - 1-Propanesulfonic Acid CAPS Buffer

Lingxian Chemical

Specifications

HS Code

362908

Chemical Formula C9H19NO3S
Molar Mass 221.317 g/mol
Appearance White to off - white crystalline powder
Pka At 25 C 10.4
Ph Range 9.7 - 11.1
Solubility Soluble in water
Buffer Capacity Good buffering capacity in its effective pH range
Stability Stable under normal conditions
Compatibility Compatible with many biological molecules
Absorbance Low absorbance in the visible and near - UV range
Packing & Storage
Packing 500 g of 3-(Cyclohexy-Lamine)-1-Propanesulfonic Acid CAPS Buffer in sealed plastic bag.
Storage Store 3-(Cyclohexylamino)-1-propanesulfonic acid (CAPS) buffer in a cool, dry place, away from direct sunlight and heat sources. Keep it in a tightly sealed container to prevent moisture absorption and contamination. It should be stored in a location separate from incompatible substances such as oxidizers and strong acids to maintain its stability and integrity.
Shipping The 3-(Cyclohexy-Lamine)-1-Propanesulfonic Acid CAPS Buffer is shipped in well - protected packaging. Ensures temperature - stable transit, safeguarding the chemical's integrity during transportation to the destination.
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3 - (Cyclohexy-Lamine) - 1-Propanesulfonic Acid CAPS Buffer
General Information
Historical Development
I tried to study the product of 3- (cyclohexylamino) -1-propanesulfonic acid (CAPS) buffer. Although this product is commonly used today, it has not been achieved overnight by tracing its source. In the past, chemical sages have worked tirelessly for many years. At first, I only knew the general idea of it, but the nature and use were mostly unknown. After repeated tests by many companies, analyzing its structure and exploring its characteristics, we knew that it can adjust acid and alkali in biochemical experiments, stabilize the environment, and has great utility. With the passage of time, the research has deepened and the application has gradually widened. It is indispensable in various fields of medical treatment and scientific research. It has finally become today's prosperous state and is a sharp tool for biochemical research.
Product Overview
"Description of 3- (cyclohexylamine) -1 -propanesulfonic acid (CAPS) buffers"
There are 3- (cyclohexylamine) -1 -propanesulfonic acid, that is, CAPS buffers, which are commonly used in chemical experiments. Its properties are stable, and the pH value can be adjusted in a specific acid-base environment to maintain the stability of the reaction system.
The appearance of the buffer is a white crystalline powder, which is easily soluble in water. When dissolved, it is evenly dispersed to obtain a clear solution. It is widely used in the fields of biochemistry and molecular biology. For example, in protein research, maintaining a suitable pH allows protein activity to be maintained and the conformation is stable. And because of its stable chemical properties, it rarely reacts with other substances in the system and does not disturb the experimental process, so it is of great importance to researchers and is indispensable in experiments to assist in scientific research and exploration.
Physical & Chemical Properties
3- (cyclohexylamine) -1 -propanesulfonic acid (CAPS) buffer is an important substance for chemical research. Its physical and chemical properties are of great value. The appearance of this substance is often white crystalline powder, and its properties are stable. From the perspective of solubility, it is easily soluble in water, which makes it easy to disperse and play a role in many aqueous experimental systems. In terms of pH, CAPS buffer has a specific buffer range, which can effectively maintain the stability of solution pH. In biochemical and chemical experiments, it can create a suitable acid-base environment for reactions. Its melting point and other physical parameters are also fixed, and these physical and chemical properties are interrelated, which together determine the application mode and effect of CAPS buffer in various chemical and biological research scenarios, which is of great significance to scientific research.
Technical Specifications & Labeling
Today, there are 3- (cyclohexylamino) -1-propanesulfonic acid (CAPS) buffers for this chemical, and its technical specifications and labels (product parameters) should be detailed.
Its properties should be clearly identifiable, and the color, morphology, etc. should be accurately described. The purity must be up to standard, and the impurity content must be strictly controlled to meet the needs of accuracy. The pH buffer range should be exact, which is a key characteristic and is related to the application effect. Stability should not be ignored. Under specific conditions, its performance should be constant. On the
label, the name should be standardized and correct, and the molecular formula and structural formula should be accurately presented. The molecular weight should be accurately marked, and the relevant physical and chemical properties should also be clearly listed. In this way, users can understand its characteristics and ensure the accuracy, safety and efficiency of the application.
Preparation Method
The method of making 3 - (cyclohexylamine) -1-propanesulfonic acid (CAPS) buffer is related to the raw materials and production process, reaction steps, and catalytic mechanism. The raw materials need to be carefully selected to ensure quality. The production process should follow a precise process. First take an appropriate amount of cyclohexylamine, and a specific ratio of 1-propanesulfonic acid, and place it in a suitable reaction vessel. Control the temperature and pressure to optimize the catalyst to promote the reaction between the two. Looking at the reaction steps, when it is gradual, do not be impatient. Pay attention to the subtle changes in the reaction process and adjust the conditions in a timely manner. The catalytic mechanism is also critical. The appropriate catalyst can quickly react and increase the yield. In this way, high-quality CAPS buffer can be obtained to meet the needs of experiments and production.
Chemical Reactions & Modifications
There is now a product called 3- (cyclohexylamino) -1-propanesulfonic acid (CAPS) buffer. As a chemical researcher, I often study the reaction and modification of these chemicals.
Looking at this CAPS buffer, its chemical reaction is related to many factors. In different temperatures, pressures and media, the reaction rate and product are different. To optimize its performance, it is necessary to investigate its reaction mechanism in detail.
As for the modification method, specific groups can be introduced to change its chemical properties. Such as modifying its molecular structure to make the buffer range more suitable, or increasing its stability to extend its use. This is all a way to improve the performance of CAPS buffers. I should explore it in depth and expect to find new gains in order to improve the properties of this product and expand its use.
Synonyms & Product Names
I have heard that there is a thing called 3- (cyclohexylamino) -1-propanesulfonic acid (CAPS) buffer. The name of its kind and the name of the product also need to be studied in detail.
This CAPS buffer has obvious functions in the field of biochemistry. The name of the same kind may be called according to its chemical structure, or according to its properties and functions. The name of the product, the merchants have their own considerations, hoping to attract attention with a unique name.
Although the name is different but the same, they are all this specific agent. Or cyclohexylaminopropanesulfonic acid buffer, or other names. The name of the merchant, or to be concise and easy to remember, or to show the characteristics. However, its essence is that it is a buffer made of 3 - (cyclohexylamino) -1-propane sulfonic acid. In experiments, production and many other things, it can exert its buffering ability and stabilize the acid and base of the environment. It is also a good aid for biochemical research and application.
Safety & Operational Standards
3- (cyclohexylamine) -1-propanesulfonic acid (CAPS) buffer product safety and operation specifications
Husband CAPS buffer is widely used in biochemical experiments. Its nature is related to the success or failure of the experiment, so safety and operation standards are essential.
#Safety matters
CAPS buffer, although not highly toxic, should be handled with caution. Touch the skin, or cause slight discomfort, if not carefully into the eyes, tingling sensation. Therefore, when handling, protective equipment is indispensable, such as gloves and goggles, to prevent accidents. And this liquid should be kept out of the reach of children to avoid accidental ingestion.
#Operation Specifications
When dosing the liquid, accurate measurement is essential. According to the concentration required for the experiment, accurately weigh the CAPS reagent, dissolve it in an appropriate amount of solvent, or water or other solutions, and stir well to ensure complete dissolution. During the dissolution process, temperature control may be required to prevent overheating or overcooling from affecting its properties.
Storage methods should not be ignored. It should be placed in a cool and dry place away from direct sunlight. Cover light, changes in temperature and humidity may damage its quality. And different batches of products may have slightly different properties. When using, read the instructions carefully.
After the experiment is completed, the remaining CAPS buffer should not be discarded at will. According to the regulations of chemical waste treatment, classified disposal to avoid polluting the environment.
In short, the handling of CAPS buffer should be based on safety and standards, so as to ensure the smooth operation of the experiment and protect the safety of people and the environment.
Application Area
The use of tasting and hearing medicines is related to the treatment of various diseases and the application of various experiments. Today there are 3- (cyclohexylamine) -1 -propanesulfonic acid (CAPS) buffers, which are widely used. In the field of biochemical experiments, it is often used for protein electrophoresis, enzyme activity measurement, etc. Because of its suitable buffer range, it can maintain the stability of the pH of the system, so that proteins, enzymes and other biomolecules can maintain their activity and conformation, and prevent them from losing their effectiveness due to pH changes.
It is also indispensable in the environment of cell culture. It can adjust the pH of the culture solution, create a habitable environment for cells, promote their growth and proliferation, and prevent external acid and base disturbances and cause cell death. And in the way of immunoassay, the antibody and antigen reactions are stable, and the accuracy and sensitivity of detection are improved, so that the results are certain. This CAPS buffer is important in the fields of biochemistry, cells and immunity, and is actually a good aid for scientific research and medical treatment.
Research & Development
The modern chemical research material, known as 3- (cyclohexylamino) -1-propanesulfonic acid (CAPS) buffer, has a wide range of experimental uses. We study its properties, preparation methods and applications, in order to make good use of it and improve its work.
At first, study the properties of CAPS buffer, which has a specific acid-base range, can stabilize the pH value of the system, and is of great benefit to biochemical and molecular biology experiments. Second, explore the method of its preparation, and apply it digitally to obtain high-purity products.
It is also used in protein electrophoresis, enzyme activity research, etc. However, there are also ways to improve, if the yield and cost reduction. We should exhaust our wisdom and promote its progress through research, hoping that this agent will further develop its potential in various fields and add new brilliance to chemical research.
Toxicity Research
The industry of chemical industry is related to people's livelihood, and the toxicity of natural substances cannot be ignored. This study of 3- (cyclohexylamine) -1 -propanesulfonic acid (CAPS buffer) is particularly important in the study of toxicity.
Guanfu CAPS buffer is commonly used in biochemical experiments. However, its toxicity is not yet known. We studied it to observe its effect on organisms. Take the white mice as a test, feed them a diet containing this substance, and after a few tens of days, observe their appearance. The white mice may have a listless state, the diet is slightly reduced, and the hair is dull.
Take the cells in the dish and apply the CAPS buffer to observe the change of the cells. It can be seen that some cells have abnormal morphology and slow proliferation. From this point of view, CAPS buffer has certain toxicity to organisms and cannot be ignored. In the future, it must be used with caution, carefully observe its dose, and take precautions to ensure the safety of experimenters and the environment.
Future Prospects
In today's world, science and technology are changing day by day, and the field of chemistry is also booming. In the field of biochemical research, 3- (cyclohexylamine) -1-propanesulfonic acid (CAPS) buffer is like a pearl, shining brightly.
This CAPS buffer has unique properties and can stabilize acid and base in a specific pH range, making it the cornerstone of many biochemical experiments. It has contributed greatly to protein research, enzyme activity measurement, etc.
Looking to the future, it is expected that its application will be more extensive. Scientists will dig deep into its potential, or shine in the field of new drug development and gene therapy. It is also expected to integrate with emerging technologies, giving rise to a new research paradigm, opening up a new situation in the field of biochemistry, and helping humans explore the mystery of life to an unprecedented level.
Frequently Asked Questions
What is the pH range of 3- (Cyclohexy-Lamine) -1-Propanesulfonic Acid CAPS Buffer?
The suitable pH range of 3- (cyclohexylamine) -1 -propanesulfonic acid (CAPS) buffer is about pH 9.7 to 11.1. This buffer has a wide range of uses in biochemical experiments, and its acid-base properties make it effective in maintaining the pH stability of the solution in this pH range.
In experiments such as protein electrophoresis, if a high pH environment is required to ensure that the protein is in a specific charge state, maintain structural stability, or promote specific reactions, CAPS buffer is extremely suitable. Because this pH range can provide a stable chemical environment, reduce the influence of external factors on the sample, and make the experimental results more reliable.
For example, when studying protein activity under alkaline conditions, the pH 10.5 environment of the CAPS buffer can simulate specific physiological or reaction conditions to ensure that the protein is at an appropriate pH level, so that the study can accurately observe its activity changes.
If some enzymatic reactions are carried out, when the reaction needs to be in a high pH environment to achieve the best catalytic efficiency, the applicable pH range of the CAPS buffer can meet the needs. By blending to a suitable pH value, the enzyme activity can be ensured to play out normally, and the experiment can be carried out smoothly.
3- (Cyclohexy-Lamine) -1-Propanesulfonic Acid CAPS Buffer is commonly used in which experiments
3- (cyclohexylamine) -1 -propanesulfonic acid (CAPS) buffer is widely used in various experiments.
In biochemical experiments, it often appears in protein and nucleic acid related research. During protein electrophoresis, CAPS buffer can create a stable pH environment, ensure the stability of protein charge state, and enable proteins to achieve good separation on the gel according to their inherent characteristics, which helps researchers accurately analyze protein molecular weight and purity. In nucleic acid extraction and analysis experiments, it can maintain the appropriate pH, prevent nucleic acid degradation, ensure the integrity of nucleic acid structure and function, and lay a solid foundation for subsequent operations such as PCR amplification and sequencing.
In cell biology experiments, cell culture is a key step, and CAPS buffer is very useful here. Cell growth is extremely sensitive to environmental pH, and CAPS buffer can effectively regulate the pH of the culture medium to meet the needs of cell growth, create a stable microenvironment for cells to survive, and greatly improve the success rate and cell activity of cell culture.
In addition, in immunochemical experiments, immunoblotting, immunoprecipitation and other techniques rely on CAPS buffer. During immunoblotting operations, it is used in membrane transfer buffer to ensure that proteins are efficiently transferred from the gel to the solid phase membrane, and during subsequent antibody incubation and detection steps, the appropriate pH is maintained to ensure the smooth progress of the antigen-antibody binding reaction, and then the target protein is accurately detected.
It can be seen that 3- (cyclohexylamine) -1-propanesulfonic acid (CAPS) buffer plays an indispensable role in many experimental fields such as biochemistry, cell biology, and immunochemistry, providing important support for the smooth development of scientific research and the acquisition of results.
3- (Cyclohexy-Lamine) -1-Propanesulfonic Acid CAPS Buffer How to configure
To prepare 3 - (cyclohexylamino) -1 -propanesulfonic acid (CAPS) buffer, you can do it according to the following method.
Prepare the required reagents and utensils first. The reagents need CAPS solute, appropriate acid-base regulator (such as hydrochloric acid, sodium hydroxide), and the utensils should have a precision balance, volumetric flask, glass rod, pH meter, etc.
Take the preparation of CAPS buffer with a certain concentration and pH value as an example. If you want to make 1L of CAPS buffer with 0.1mol/L and pH 10.0, first weigh an appropriate amount of CAPS solute with a balance. According to its molar mass, 0.1mol/L × 1L needs to be weighed and placed in a beaker. Add an appropriate amount of deionized water and stir with a glass rod to help dissolve. After
, transfer this solution to a 1L volumetric flask, rinse the beaker several times with a small amount of deionized water, and then add deionized water to the scale line.
Next, monitor with a pH meter and slowly adjust the pH with an acid-base regulator. If the pH is low, add sodium hydroxide solution dropwise; if the pH is high, add hydrochloric acid solution dropwise to pH 10.0. Finally, bandwidth evaluation to the scale line, shake well to obtain the required CAPS buffer. During the process, pay attention to the operating specifications to ensure the accuracy and reliability of the buffer.
How stable is the 3- (Cyclohexy-Lamine) -1-Propanesulfonic Acid CAPS Buffer?
3 - (hexylamine) -1 -propanesulfonic acid (CAPS) liquid is of high quality. This liquid can be stored for a short time under normal conditions, and its properties can still be maintained. However, if you want to survive for a long time, you need to be cautious.
Under normal circumstances, if the CAPS liquid is stored in the room for 7 days, its pH value and chemistry may not change. However, if the environment is too high and the storage is too high, some components in the liquid may be accelerated due to the reaction, causing its equilibrium to change, and its quality will also be reduced.
Furthermore, light also affects its quality. If the CAPS liquid is exposed to light, or it may cause photochemical reactions, it will break the original transformation of the liquid and affect its performance.
To maintain good quality of the CAPS liquid, it is recommended to store it in a warehouse and protected from light. If it needs to be stored for a long time, it can be refrigerated, generally at 2-8 ° C. In this way, it can effectively extend its storage time, ensure that it is in use, and can maintain good performance to meet the needs of each warehouse or project.
What is the effect of 3- (Cyclohexy-Lamine) -1-Propanesulfonic Acid CAPS Buffer on biomolecules?
3- (hexylamine) -1-propanesulfonic acid (CAPS) solution is used in the study of biomolecular effects. The pH effect of the solution is 9.7-11.1, which is used for the study of specific biomolecules such as lyophilic enzymes.
On the protein surface, due to its specific pH environment, it can effectively maintain protein stability and prevent protein stability. For example, in some protein purification processes that require high pH environment, the CAPS solution can maintain the integrity of the protein, ensuring that its activity is not lost.
In the field of nucleic acid research, high pH environment may affect the effect of nucleic acid groups. The properties provided by CAPS solution may promote DNA differentiation. This property is very important for specific nucleic acid analysis techniques, such as coagulation, etc., and researchers can use it to explore the properties of nucleic acids.
In addition, CAPS solution does not react to many biological molecules due to its chemical properties. Therefore, in biological analysis techniques such as immunoassay and enzyme immunosorbent assay (ELISA), it can also be used to ensure that the specific properties of biomolecules are not dried and improve the sensitivity. Therefore, CAPS solution plays an indispensable role in many aspects of biomolecular research according to its specific pH and chemical characterization.