3-Cyclohexylamino-1-Propanesulfonic Acid

3-Cyclohexylamino-1-propanesulfonic acid is often used as a biological buffer and has a wide range of uses in biochemical and medical fields.
In biochemical experiments, it is often selected because it can effectively maintain the stability of the pH value of the system. Many chemical reactions in organisms are extremely sensitive to pH, and slight changes may affect the reaction rate and even direction. This substance can create a suitable pH environment to ensure the normal structure and function of biomolecules. For example, in the study of enzyme activity, different enzymes have their best pH range. By adjusting the buffer system with 3-cyclohexylamino-1-propanesulfonic acid, the enzyme can be at the optimum pH, and the enzyme characteristics and catalytic mechanism can be accurately explored.
At the medical diagnostic level, it plays a key role in clinical test reagents. Such as common blood and urine detection reagents, in order to ensure accurate and reliable test results, the pH of the reaction system needs to be strictly controlled. The substance can maintain a stable pH environment, allow the detection chemical reaction to proceed smoothly, ensure accurate results, and help doctors accurately diagnose the disease.
In the field of cell culture, cell growth has strict environmental requirements, and pH stability is essential. 3-Cyclohexylamino-1-propanesulfonic acid can adjust the pH of the medium, create an acid-base environment similar to that in the body for cells, and support normal cell growth, proliferation and metabolism. It is an indispensable substance for large-scale cell culture in cell biology research and biopharmaceutical processes.

3 - Cyclohexylamino - 1 - Propanesulfonic Acid, Chinese name 3 - cyclohexylamino - 1 - propanesulfonic acid, often abbreviated as CAPS. This is an important biological buffer, its physical properties are as follows:
In appearance, it is mostly white crystalline powder with fine texture, like snow falling in the early winter, pure and free of impurities. This pure appearance is conducive to accurate control of dosage in various experimental and production applications, and avoids deviation of experimental or production results due to impurity interference.
Solubility is quite good, soluble in water. When dissolved in water, it is like fine sand merging into a torrent, quickly and evenly dispersed to form a uniform and stable solution. Its good solubility in water facilitates many biochemical reactions that need to be carried out in an aqueous environment, allowing the reactants to be fully contacted to ensure the smooth progress of the reaction. The melting point range is 300-305 ° C, and the melting point is quite high. This characteristic indicates that the substance has a relatively hot topic stability. In a relatively high temperature environment, the structure and properties can still remain stable, and it is not easy to decompose or undergo chemical changes due to heat. Just like a solid fortress, it remains immovable under the test of high temperature, ensuring reliability in experiments or production processes involving high temperature operation.
In addition, its pH buffer range is about 9.7 - 11.1. Within this pH range, it can effectively resist the influence of adding a small amount of acid, alkali or dilution on the pH value of the solution and maintain the pH value of the solution. Like a loyal guardian, always guarding the pH environment of the solution, escorting those biochemical reactions that are extremely sensitive to pH changes, ensuring that the reaction proceeds efficiently and orderly under suitable pH conditions.

3-Cyclohexylamino-1-propanesulfonic acid, which is a commonly used reagent for biochemical experiments, must pay attention to many matters when storing and transporting.
Let's talk about storage first. Because of its nature, it is easy to be influenced by the environment, so it should be placed in a cool, dry and well-ventilated place. Avoid light and shade to prevent light from causing it to decompose or deteriorate. If stored in a high temperature and humid place, or in excessive contact with moisture, it may affect its chemical stability and cause quality to decline. Furthermore, ensure that the storage container is tightly sealed to prevent mixing with air, water vapor and other impurities, thereby maintaining its purity.
As for transportation, relevant regulations and standards must be followed. Because it may have certain chemical activity, proper protective measures must be taken during transportation. The packaging must be firm and reliable to prevent the leakage of reagents due to damage to the container. At the same time, care should be taken to avoid violent vibration and collision to prevent damage to the packaging. The transportation environment should also maintain suitable temperature and humidity, and should not be exposed to extreme conditions, so as to ensure that the quality of 3-cyclohexylamino-1-propanesulfonic acid during transportation is not affected, and it can still maintain good chemical properties when it arrives at the destination, so that it can be suitable for subsequent experiments or production needs.

3-Cyclohexylamino-1-propanesulfonic acid, often referred to as CAPS, is synthesized as follows:
First take an appropriate amount of cyclohexylamine, place it in a reactor, and slowly inject a certain amount of 3-chloro-1-propanesulfonic acid. This process requires strict temperature control, and an ice-water bath or other temperature control device is used to maintain a low temperature environment. Because high temperature is prone to side reactions, it affects the purity and yield of the product.
Then, add an appropriate amount of acid binding agent, such as triethylamine, which aims to neutralize the hydrogen chloride generated during the reaction and promote the positive progress of the reaction. The dosage of acid binding agent should be precisely prepared according to the proportion of the reactants, so as not to make excess or insufficient, so as not to affect the chemical equilibrium of the reaction.
When reacting, stir at a moderate speed to promote full contact of the reactants and uniform reaction. After several hours or even dozens of hours of reaction, the reaction process should be monitored by means of analysis methods such as thin layer chromatography or high performance liquid chromatography. When the raw material point is almost gone, it is regarded as the end of the reaction.
After that, the reaction mixture is poured into the separation funnel and extracted with a suitable organic solvent. The choice of organic solvent depends on the difference in solubility between the product and the impurities, and must be immiscible with the water phase. After multiple extractions, the product is enriched in the organic phase.
The resulting organic phase is extracted and dried with a desiccant such as anhydrous sodium sulfate or magnesium sulfate to remove the remaining moisture. Subsequently, the organic solvent is removed by rotary evaporation to obtain a crude product.
The crude product needs to be further purified, and the method of recrystallization can be used. Choose an appropriate solvent to dissolve the crude product at a higher temperature. After cooling, the product crystallizes and precipitates, and impurities remain in the mother liquor. Filter and collect crystals, and dry at low temperature to obtain a pure 3-cyclohexylamino-1-propanesulfonic acid product. The entire synthesis process must follow the standard operation of chemical experiments, pay attention to safety and environmental protection, and obtain high-quality products.

3-Cyclohexylamino-1-propanesulfonic acid, the price in the market, it is difficult to say for sure. According to the method of Guanfu's "Tiangong Kaiwu", when it comes to the price of things, it often varies with time, place, and quality. The same is true for this acid.
At that time, if the supply is abundant during the peak season of production, the price may tend to be flat; if the supply is scarce during the time of famine, the price will surge.
At the place of origin, the price is very cheap, covering no transshipment fees or storage costs. And far from the place of origin, with the addition of freight and taxes, the price will increase.
As for the quality, those with high purity and excellent quality will have a price higher than that of ordinary products. To determine the price, one must carefully examine the supply and demand of the city, the quality of the quality, and the time of the place. The state of the city is changing rapidly, and it cannot be hidden by a word. Or consult the merchants of pharmaceutical companies, or observe the chemical market, to get a near-real price. Although it is difficult to determine the exact number, according to the common conditions of the city, the price varies from a gram or a few yuan to a few dozen yuan. This is an approximate number and is for reference only. The truth is still subject to the current market conditions.