3-(Cyclohexylamino)-2-Hydroxy-1-Propanesulfonic Acid

3- (Cyclohexylamino) -2-hydroxy-1-propane sulfonic acid (3- (Cyclohexylamino) -2-Hydroxy-1-Propanesulfonic Acid), often referred to as CAPSO, is an important biological buffer. Its chemical structure can be viewed as follows:
The main chain of this compound is propane structure, and there is a sulfonic acid group (-SO 🥰 H) attached to the 1-position of propane, which gives the substance a certain degree of acidity and water solubility. There is a hydroxy group (-OH) at the 2-position, which is hydrophilic and can participate in the formation of hydrogen bonds, which has a great influence on the solubility of compounds and intermolecular interactions. The 3-position is connected to the cyclohexyl amino group (-NH - C H). The cyclohexyl group is a cyclic structure with certain rigidity and hydrophobicity, and the amino group (-NH -) can participate in the acceptance and release of protons, which is of great significance to its buffering performance.
Overall, the chemical structure of 3- (cyclohexylamino) -2-hydroxy-1-propanesulfonic acid integrates functional groups with different characteristics. The interaction between sulfonic acid groups, hydroxy groups, and cyclohexylamino groups makes it have unique physical and chemical properties, especially in the pH buffer interval required for biochemical experiments. It can play a stable buffering role, so it is widely used in biochemical research, diagnostic reagents and other fields.

3- (cyclohexylamino) -2-hydroxy-1-propanesulfonic acid, often referred to as CAPSO, is a biological buffer. Its main application fields are quite extensive, as detailed below:
In biochemistry and molecular biology experiments, this agent has a wide range of uses. Many biochemical reactions in organisms are extremely sensitive to environmental pH, and small changes may affect the reaction process and biological macromolecule activity. CAPSO has good buffering ability, which can stabilize the pH value of the reaction system within a specific range, ensuring the smooth development of enzymatic reactions, protein purification and analysis, and nucleic acid research. For example, in some enzyme activity measurement experiments that require strict pH value, it is possible to accurately determine enzyme activity and explore its kinetic parameters by building a stable pH environment with CAPSO.
The field of cell culture is also indispensable. Cell growth has strict requirements on the pH value of the microenvironment in which it is located. CAPSO can effectively stabilize the pH value of cell culture medium, create a suitable environment for cell growth, reduce the adverse effects of pH fluctuations on cell growth, metabolism and function, and help cell culture experiments to advance stably and improve the success rate and quality of cell culture.
In the field of clinical diagnosis, this agent also plays an important role. In many diagnostic detection methods such as biochemical analyzer detection, immunoassay, etc., it is necessary to precisely control the pH value of the reaction system to ensure the accuracy and reliability of the detection results. As a buffer, CAPSO can ensure that the diagnostic reagents are at the appropriate pH conditions, so that the detection reaction can be carried out efficiently, thus providing accurate data support for disease diagnosis.
In the process of drug development, CAPSO also plays a key role. Drug development involves many experimental links, such as drug stability research, drug formulation optimization, etc., all of which need to control the pH value of the system. CAPSO can simulate the physiological pH environment of the human body, help to study the stability, solubility and interaction of drugs with other components under different pH conditions, and provide key references for drug dosage form design, prescription screening and quality control, and promote the drug development process.

3- (cyclohexylamino) -2-hydroxy-1-propanesulfonic acid, often referred to as CHAPS, is a surfactant with zwitterionic properties and is widely used in the field of biochemical research. Its physicochemical properties are as follows:
- ** Properties **: Usually presented as a white crystalline powder, it is pure and uniform in texture, which is convenient for accurate weighing and experimental operation.
- ** Solubility **: This substance exhibits good solubility in water and can be rapidly and uniformly dispersed between water molecules to form a uniform and stable solution. At the same time, it also has a certain solubility in organic solvents such as methanol, which makes it suitable for a variety of experimental systems and reaction environments.
- ** Melting point **: The melting point of this substance is about 190-195 ° C. In this temperature range, the substance transitions from solid to liquid. This characteristic is of great significance for experimental operations involving temperature control, such as heating reactions, separation and purification.
- ** pH Buffering Range **: CHAPS has a certain pH buffering capacity, and its effective buffering range is about pH 6.5 - 8.5. Within this pH range, it can effectively resist the violent change of pH value caused by the addition of a small amount of acid or base, thus providing a stable acid-base environment for many biochemical reactions and experimental systems that require strict pH conditions.
- ** Critical Micelle Concentration (CMC) **: This is a key parameter to measure the characteristics of surfactants. The critical micelle concentration of CHAPS is relatively low, about 6.6 mM. When the concentration of CHAPS in the solution reaches or exceeds this value, the surfactant molecules will spontaneously aggregate to form a micelle structure. This property is widely used in the fields of protein solubilization and membrane protein research. It can be used to simulate the biofilm environment with the help of micelle structure to effectively maintain the natural conformation and biological activity of membrane proteins.

3- (cyclohexylamino) -2-hydroxy-1-propanesulfonic acid, this is a commonly used buffer for biochemical experiments. During storage and transportation, many key matters need to be paid attention to.
First, the storage temperature is very important. It should be stored in a cool and dry place, generally refrigerated at 2-8 ° C. If the temperature is too high, or its chemical properties change, the buffering performance will be reduced; if the temperature is too low, it may freeze and affect the use.
Second, moisture resistance is also the key. Because it has a certain water absorption, it may decrease the purity after being damp, which will affect the experimental effect. Therefore, be sure to ensure that the storage container is well sealed and avoid exposure to humid environment.
Third, during transportation, it is necessary to prevent violent vibration and collision. Although the chemical properties of this substance are relatively stable, violent vibration may damage the packaging, which will affect its quality.
Fourth, pay attention to isolation from other substances. Do not mix with oxidants, acids, alkalis, etc., because or chemical reactions with these substances, not only damage themselves, but also may cause safety accidents.
Fifth, the storage period cannot be ignored. It should be used within the specified shelf life. If it is overdue or due to changes in ingredients, the buffering capacity will be reduced or other unpredictable conditions will occur.
In short, proper storage and transportation of 3- (cyclohexylamino) -2-hydroxy-1-propanesulfonic acid can ensure its quality and performance, and provide a solid guarantee for the smooth development of the experiment.

3- (cyclohexylamino) -2-hydroxy-1-propane sulfonic acid, often abbreviated as CAPSO, is an important biological buffer. Its synthesis method is quite critical, which is related to its application in biochemical research and other fields. In ancient Chinese, the synthesis method is as follows:
The classic route is first introduced, using a propanol derivative containing a hydroxyl group and a sulfonic acid group as the starting material. First, the raw material undergoes a nucleophilic substitution reaction with cyclohexylamine. In a suitable reaction vessel, accurately measure the raw material and cyclohexylamine and put it in a certain molar ratio. Add an appropriate amount of catalyst, such as some organic bases, to promote the reaction. Adjust the reaction temperature and duration, usually control the temperature in a moderate range, such as between 40 and 60 degrees Celsius, and react for several hours (several hours) to make the two fully react. After the reaction is completed, the separation and purification method, such as column chromatography, removes the unreacted raw materials and by-products, and obtains a relatively pure 3- (cyclohexylamino) -2 -hydroxy-1-propanesulfonic acid.
Another improved method is to protect the hydroxyl group of the starting material first to prevent unnecessary side reactions during the reaction. Commonly used protective groups, such as tert-butyl dimethyl silyl, etc. The protected raw material reacts with cyclohexylamine, and the reaction conditions are similar to the above classical method. After the reaction is completed, the deprotection step is carried out. Appropriate reagents are used to remove the protective group under mild conditions to obtain the target product. This improved method can improve the purity and yield of the product, but the operation is more complicated and each step needs to be carefully controlled.
Furthermore, there are also ways to synthesize through multi-step reactions using other related compounds as starting materials. If a specific olefin derivative is used as the starting point, the addition reaction is first carried out to introduce the sulfonic acid group, and then a series of reactions such as cyclization and amination are carried out to finally synthesize 3- (cyclohexylamino) -2-hydroxy-1-propane sulfonic acid. Although there are many steps in this route, if the reaction conditions of each step are properly optimized, ideal results can be obtained.