What are the chemical properties of 3-Cyclohexylamino-Propane-1-Sulfonic Acid?
3-Cyclohexylamino-propane-1-sulfonic acid is an organic compound with stable properties and is commonly found in biochemical studies.
This substance is solid at room temperature, appears or is a white crystalline powder, has certain solubility, is soluble in water, and can form a uniform solution. This property makes it very convenient in experimental operation and reaction system construction, which can ensure its effective participation in biochemical reactions.
It is acidic, and the sulfonic acid group can release protons in the solution, making the solution acidic. This acidic property is crucial in the regulation of the acid-base environment of many biochemical reactions. It can act as a buffer component to maintain the stability of the pH value of the reaction system and ensure the normal structure and function of biological macromolecules (such as proteins and nucleic acids).
In chemical reactions, its cyclohexyl and amino groups are also of great significance. Cyclohexyl is a rigid cyclic structure, which can provide spatial resistance and hydrophobicity, and affect the spatial configuration of molecules and interact with other substances. Amino groups have certain nucleophilic properties and can participate in reactions such as nucleophilic substitution. They play a role in organic synthesis and biochemical labeling. Due to its structure containing a variety of active groups, it has shown wide application potential in many fields such as medicinal chemistry and biochemical analysis, and can provide key chemical tools for new drug development and biomolecular detection.
What are the main uses of 3-Cyclohexylamino-Propane-1-Sulfonic Acid?
3 - Cyclohexylamino - Propane - 1 - Sulfonic Acid, or 3 - cyclohexylamino propane sulfonic acid, is an important biochemical reagent. Its uses are quite extensive and are described as follows:
In the field of biochemistry and molecular biology experiments, this reagent is often used as a buffer. Because it can effectively maintain the stability of the pH value of the solution, it can ensure that various biochemical reactions can proceed smoothly in a suitable pH environment. Many enzymatic reactions are extremely sensitive to pH values. If there is a slight deviation, the activity of enzymes will be affected. The stable buffer system constructed by 3-cyclohexylaminopropane sulfonic acid is like a stable "hotbed" for enzymatic reactions, so that the reaction process is not disturbed by pH fluctuations, thus ensuring the accuracy and reliability of experimental results.
is also indispensable in the study of proteins and nucleic acids. The structure and function of proteins and nucleic acids are highly susceptible to changes in environmental pH. Using this reagent to adjust and maintain a suitable pH value can maintain the natural structure and activity of proteins and nucleic acids. For example, in protein crystallization experiments, precise control of pH value is a key factor in obtaining high-quality protein crystals. 3-cyclohexylaminopropane sulfonic acid plays an important role in this process, enabling researchers to deeply explore the fine structure of proteins and lay the foundation for revealing the mysteries of life.
In the field of cell culture, it is also of great value. Cell growth requires strict environmental pH values, and an appropriate pH range is essential for the normal metabolism and proliferation of cells. Adding this reagent to the cell culture fluid can maintain the pH value of the culture fluid and create a suitable growth environment for cells, just like creating a comfortable "home" for cells, ensuring the smooth development of cell culture experiments. It is of great significance to cell biology research and biopharmaceutical industries.
In summary, 3-cyclohexylaminopropane sulfonic acid plays a crucial role in many scientific research fields such as biochemistry, molecular biology, cell biology, and related industries, and has made significant contributions to the development of life sciences.
3-Cyclohexylamino-Propane-1-Sulfonic Acid is widely used in which areas
3-Cyclohexylamino-propane-1-sulfonic acid, its English name is 3-Cyclohexylamino-Propane-1-Sulfonic Acid, often referred to as CAPS. This substance is widely used in many fields such as biochemistry and medical research.
In the field of biochemical experiments, it is a class of extremely important biological buffers. Because it has a specific pH buffer range, about pH 9.7-11.1, it can effectively maintain the stability of solution pH in this range. For example, in the research experiments of biological macromolecules such as proteins and nucleic acids, the structure and function of many biological macromolecules are extremely sensitive to environmental pH. CAPS can create a suitable pH environment to ensure the structural stability and biological activity of these biological macromolecules, so that relevant experiments can be carried out accurately. Just like in some enzymatic reaction experiments, specific enzymes need to exhibit the best activity under specific pH conditions, CAPS can provide a stable pH environment for these enzymatic reactions, enabling researchers to accurately explore the characteristics and reaction mechanisms of enzymes.
In the field of pharmaceutical research and development, CAPS is also of great significance. In the development process of pharmaceutical preparations, in order to ensure the stability and effectiveness of drugs, the pH value of the preparation needs to be strictly controlled. CAPS can act as a buffer to adjust the pH of drug preparations and ensure the stability of drugs during storage and use. Just like some injections, a precise pH environment is required to prevent drug deterioration and precipitation. CAPS can play a key role in maintaining the quality and stability of injections, thereby ensuring drug safety and efficacy.
In addition, in molecular biology experiments such as gene sequencing and PCR amplification, a suitable pH environment is of great significance to the smooth progress of the reaction and the accuracy of the results. As a buffer, CAPS can ensure the pH stability of the reaction system, laying the foundation for the successful implementation of these experiments, just like calibrating environmental parameters for precision experimental instruments, making the experimental results more reliable and accurate.
What is the preparation method of 3-Cyclohexylamino-Propane-1-Sulfonic Acid?
The method for preparing 3-cyclohexylamino-propane-1-sulfonic acid is described in past books. The method is as follows:
First take an appropriate amount of 3-chloropropane-1-sulfonic acid and place it in a clean reactor. Take cyclohexylamine and slowly add it to the kettle in a certain proportion. The ratio of the two needs to be accurately measured, which is related to the effectiveness of the reaction. If the ratio is not correct, the product is impure or the yield is low.
In the reactor, adjust the temperature to a suitable environment. Generally speaking, it is appropriate to control the temperature at [X] ° C. If the temperature is too high, side reactions will occur frequently, and the product will be difficult to purify; if the temperature is too low, the reaction will be slow and take a long time. Control the temperature in a water bath or an oil bath to ensure that the temperature is constant.
At this temperature, stir evenly. The stirring rate also needs to be appropriate. If it is too fast, the energy consumption will increase greatly. If it is too slow, the reactants will be unevenly mixed and the reaction will be difficult to be sufficient. Make the two fully contact, and a nucleophilic substitution reaction will occur. After several hours, the reaction will be completed asymptotically.
After the reaction is completed, the reaction liquid will be poured out and extracted with an appropriate solvent. It is often extracted with an organic solvent such as [specific name of organic solvent] to remove impurities. After distillation and concentration, the solvent
The crude product needs to be further purified. The method of recrystallization is preferred, and a suitable solvent is selected, such as [name of specific recrystallization solvent]. Dissolve the crude product in a hot solvent, wait for cooling, and the pure 3-cyclohexylamino-propane-1-sulfonic acid crystallizes out. The crystals are collected by filtration and dried at low temperature to obtain a pure product. This preparation method and careful handling of each step can obtain high-quality products.
3-Cyclohexylamino-Propane-1-Sulfonic the market price of Acid
3 - Cyclohexylamino - Propane - 1 - Sulfonic Acid, 3 - cyclohexylamino propane sulfonic acid, often referred to as CAPS, is a commonly used buffer in biochemical research. Its market price varies depending on quality, purity, packaging specifications and suppliers.
Looking at the market conditions in the past, for high-purity products with a purity of 99%, if the packaging is 100 grams, the price is about 80 to 120 yuan. Due to the complicated preparation process of high-purity products and strict requirements on raw materials and production environment, the cost is high and the price is not cheap.
If the packaging is increased to 500 grams, the price is roughly in the range of 300 to 500 yuan. This is because when the quantity is large, the cost of packaging, transportation, etc. is reduced, and the unit price will decrease.
As for products with slightly lower purity, such as 98% purity, the price of 100 grams of packaging may be around 60 to 90 yuan. Because of its slightly lower purity, the production difficulty and cost are slightly reduced, so the price is also reduced.
There are also small packages, such as 25 grams, with 99% purity, the price is about 30 to 50 yuan. This package is suitable for those who only need a small amount of experimental exploration. Although the unit price is higher, it is better than accurate dosage and cost control.
Furthermore, different suppliers have different prices due to different brand influence, production scale and sales strategies. Well-known brands have strict quality control and good reputation, and their prices may be slightly higher than ordinary brands. However, buyers need to comprehensively consider price and quality to choose the most suitable product.