3-(Cyclohexylamino)Propane-1-Sulfonic Acid

3- (cyclohexylamino) propane-1 -sulfonic acid is often used as a biological buffer and has a wide range of uses in biochemical research. It has many characteristics and is suitable for specific scenarios.
For protein and nucleic acid research, it can stabilize the pH value of the environment. Proteins and nucleic acids are sensitive to pH, and small changes can affect the structure and function. This substance can maintain a stable pH to ensure normal research. For protein crystallization experiments, precise pH is the key. It can create a suitable environment to promote crystallization and help resolve protein structures.
Enzyme activity research is also commonly used. Enzymes are best active in a specific pH range, and they can control the pH of the system, allowing enzymes to exert the best activity, which is convenient for studying enzyme kinetics and mechanism of Like the determination of amylase activity, use it to maintain pH and accurately measure the hydrolysis of starch by enzymes.
In the field of cell culture, create a stable microenvironment for cells. Cell growth requires strict pH, which can prevent pH fluctuations from damaging cells and ensure normal cell growth and metabolism. In animal cell culture, adding this substance can improve cell survival and growth state.
As a buffer component in electrophoresis experiments, it ensures pH stability during electrophoresis, and effectively separates biological macromolecules according to charge and size. Nucleic acid electrophoresis, stabilizing pH guarantees the separation effect of nucleic acid fragments, which is conducive to analysis and detection.
In summary, 3- (cyclohexylamino) propane-1 -sulfonic acid is indispensable in many fields of biochemical experiments, and is of great significance to obtain accurate and reliable experimental results.

3 - (cyclohexylamino) propane-1 -sulfonic acid is a commonly used biological buffer. Its physical properties are quite unique, let me tell you one by one.
Looking at its shape, under room temperature and pressure, it is mostly in the shape of white crystalline powder, with a fine texture, like the first snow in winter, pure and simple. This state is easy to use and store, and it is also conducive to subsequent experimental operations.
When it comes to solubility, it shows good solubility in water. Just like a fish entering water, it quickly melts with water to form a uniform and stable solution. This property allows it to be easily formulated into a buffer of the required concentration in many biochemical experiments and industrial production, creating a suitable acid-base environment for the reaction.
Furthermore, the melting point is also one of its important physical properties. After precise determination, its melting point is within a specific range. This value is the inherent property of the substance, just like the unique fingerprint of each person, which can help identify and determine the purity. The precise melting point also reflects the stability and regularity of the structure of the substance.
Its density also has a specific value. Although it is invisible and colorless, it builds a bridge between the quantity and volume of the substance. The stability of the density ensures the accurate measurement of the quantity of the substance in different batches of production and use, laying the foundation for the accuracy of experiments and production.
In addition, 3- (cyclohexylamino) propane-1 -sulfonic acid also has a certain chemical stability. Under normal experimental conditions and storage environments, it can maintain its own structure and properties unchanged for a long time. However, just as everything in the world has its limits, its structure and properties may also change when exposed to special conditions such as extreme temperatures, pH or strong oxidants.
In summary, the physical properties of 3- (cyclohexylamino) propane-1 -sulfonic acid, such as morphology, solubility, melting point, density and chemical stability, are interrelated and together determine its wide application and important position in the field of biochemistry.

3- (cyclohexylamino) propane-1 -sulfonic acid, this material is stable, in common temperature environments, can persist for a long time without melting. In its structure, cyclohexyl is as stable as a rock, and the structure of the ring makes it chemically blunt, and it should slow down with other substances. Although the sulfonic acid group is alive, its stability is increased by the whole state of the molecule.
At room temperature, if it is protected from water and fire, strong acid and alkali and strong oxidative reducing agents, it will be safe like Mount Tai. At high temperatures, or from decomposition, the sulfonic acid group gradually cracks, generating sulfur-containing gas and other small molecules. Strong acid and alkali are also restless, and both the sulfonic acid group and the amino group should react to its structure and change its essence.
Under light, the molecule can absorb light, call the electron jump, or induce chemical changes, but usually under light intensity, it changes very little. In humid atmosphere, due to the hydrophilicity of the sulfonic acid group, or a little moisture absorption, it will not greatly improve the physical properties.
In general, 3- (cyclohexylamino) propane-1 -sulfonic acid is stable under general conditions. However, in special circumstances, such as high temperature, strong acid and alkali, strong light and high humidity, or changes, it should be used for storage. When the environment is carefully inspected to ensure its purity and stability.

3- (cyclohexylamino) propane-1 -sulfonic acid, often referred to as CAPS, is a zwitterionic buffer frequently used in biochemical research. The synthesis method is as follows:
Starting materials, acrylic acid and cyclohexylamine are selected as the main ones. Acrylic acid has the active groups of alkenyl and carboxyl groups; cyclohexylamine contains nitrogen atoms and can be introduced into amino groups. These two are the basis for synthesis.
Addition reaction of acrylic acid and cyclohexylamine is performed first. The alkenyl group of acrylic acid can be nucleophilically added to the amino group of cyclohexylamine with the help of suitable temperature and catalyst. This reaction needs to be controlled at temperature, generally between room temperature and 50 ° C. If the temperature is too high, it may cause side reactions and cause impure products. The catalyst can be selected as an organic base, such as triethylamine, which can promote the reaction and speed up the reaction rate. During the reaction, the two are put in a molar ratio of 1:1, stirred thoroughly to make them contact evenly, and the reaction time is about 2-4 hours until the pH value of the reaction solution is stable, indicating that the addition reaction is generally complete.
The addition product is then sulfonated with sodium bisulfite. Sodium bisulfite provides a sulfonic acid group and is integrated into the molecule. This reaction needs to be carried out in an aqueous solution, and the temperature should be controlled at 60-80 ° C. During the reaction, sufficient stirring is required to ensure the uniformity of the material. The molar ratio of sodium bisulfite to the addition product is about 1.2:1. A slight excess of sodium bisulfite can promote the reaction to move in the direction of generating the target product. The reaction time is about 3-5 hours. The reaction progress is monitored by thin layer chromatography. When the raw material point disappears, the sulfonation reaction is completed.
After the reaction is completed, the product needs to be separated and purified. First, water and low boiling point impurities in the reaction system are removed by vacuum distillation. Then, an organic solvent extraction, such as ethyl acetate, can remove unreacted raw materials and some by-products. The extract was then dried by anhydrous sodium sulfate, the desiccant was filtered out, and then separated by column chromatography. Silica gel was used as the stationary phase, and the mixture of petroleum ether and ethyl acetate was used as the mobile phase. According to the polarity of the product, it was separated from impurities. Finally, pure 3- (cyclohexylamino) propane-1-sulfonic acid was obtained.

3- (cyclohexylamino) propane-1 -sulfonic acid, often known by its abbreviation CAPS, is widely used in many fields such as biochemical research.
It is an indispensable buffer in the field of biochemical experiments. In the study of biological macromolecules such as proteins and nucleic acids, it can create and maintain a stable pH environment. Because of the structure and function of proteins and nucleic acids, it is extremely sensitive to the pH of the environment. Appropriate pH conditions are the key to ensuring that biological macromolecules maintain their natural conformation and biological activity. For example, in protein electrophoresis experiments, the CAPS buffer system can precisely regulate the pH of the electrophoresis solution, so that proteins can be separated efficiently according to their charge and molecular weight differences, providing clear and accurate results for protein analysis.
In the biopharmaceutical industry, it also plays an important role. In the process of drug development and production, many biological agents require a specific pH environment to maintain their stability and activity. CAPS can be used to prepare drug buffers to ensure that the quality and efficacy of drugs are not affected by pH fluctuations during storage and use. For example, some enzyme drugs need to maintain their activity in a specific alkaline environment, and CAPS can create suitable conditions for them.
In the field of cell culture, there are also many figures. Cell growth has strict pH requirements in the culture environment. CAPS can help maintain the pH stability of the culture medium, create a good growth microenvironment for cells, and help normal cell metabolism and proliferation. It is of great significance in cell biology research and biological product production.
In the field of environmental monitoring, 3- (cyclohexylamino) propane-1-sulfonic acid is also useful. When detecting some pH-sensitive substances in environmental samples, its buffering properties can be used to stabilize the pH of the sample, avoid deviation of detection results due to pH changes, and improve the accuracy and reliability of environmental monitoring data.