3-Cyclohexylaminopropanesulfonic Acid

3-Cyclohexylaminopropane sulfonic acid has a wide range of uses. In the field of biochemical research, it is an important buffer. The biochemical reaction is extremely sensitive to the environmental pH, and subtle pH changes may cause reaction imbalance and biomolecular activity changes. And 3-cyclohexylaminopropane sulfonic acid can effectively maintain the pH stability of the reaction system and make the biochemical reaction proceed smoothly. Experiments such as enzymatic reactions and protein crystallization depend on it to create a suitable acid-base environment.
In the pharmaceutical and chemical industry, it is also indispensable. In the pharmaceutical process, the synthesis, purification and preparation of drugs often require precise control of pH. 3-Cyclohexylaminopropane sulfonic acid, with its good buffering properties, ensures that the pH of all links of drug production meets the requirements, which is of great significance to ensure the quality and stability of drugs.
And it also plays a key role in the preparation of clinical diagnostic reagents. The performance of diagnostic reagents is closely related to pH. 3-cyclohexylaminopropane sulfonic acid can maintain a stable pH of reagents, improve the accuracy and reliability of test results, and help medical workers accurately diagnose diseases.
In summary, 3-cyclohexylaminopropane sulfonic acid has important uses in many fields such as biochemistry, pharmaceutical chemistry, and clinical diagnosis. It is an important chemical substance to promote the development of related industries.

3-Cyclohexylaminopropanesulfonic acid, its physical properties are as follows:
This substance is mostly white crystalline powder at room temperature, with pure color and no variegated spots. Its texture is delicate, smooth to the touch, and uniform like fine sand.
In terms of solubility, it exhibits good solubility in water, and can be miscible with water in a suitable ratio, just like a fish entering water, quietly fusing to form a uniform and stable solution. In some polar organic solvents, there is also a certain solubility, but compared with water, the degree of solubility may be different.
In terms of melting point, it has a specific melting point value. When heated to this temperature, the substance gradually changes from a solid state to a liquid state. This process is smooth and orderly, like melting snow and melting, showing the orderly changes in the internal structure of the substance due to heat.
Its density is also an inherent physical constant, reflecting the characteristics of the mass contained in the unit volume of the substance, which is like a scale to measure its compactness, giving it a unique physical occupancy property in a specific environment.
In addition, the powder of this substance has good fluidity, easy to dump and transfer during storage and transportation, and will not agglomerate easily, ensuring its smooth application in industrial production and experimental operations. It is like a smart quicksand that flows freely.

3-Cyclohexylaminopropanesulfonic acid, often referred to by its English abbreviation CAPS. This substance is widely used in the fields of biochemistry and molecular biology.
In protein research, protein electrophoresis often requires a specific pH environment to ensure protein stability and maintain its charge characteristics. CAPS is often used in the preparation of protein electrophoresis buffers because it can create a stable alkaline environment. For example, when separating basic proteins, the buffer system containing CAPS can provide suitable conditions to effectively separate proteins according to molecular weight and charge differences, enabling researchers to further explore protein properties.
Enzymatic research is also common. The activity of enzymes is closely related to the pH of the environment in which they are located. CAPS can be used to build an enzymatic reaction buffer system to stabilize the pH of the reaction environment and keep the enzyme in the best active state. Like some enzymes that function under alkaline conditions, the stable environment created by CAPS can ensure the smooth progress of enzymatic reactions, which is convenient for researchers to accurately determine enzyme activities and kinetic parameters.
In the field of cell culture, cell growth has strict environmental requirements and pH stability is essential. CAPS can be used in the cell culture medium buffer system to maintain the pH of the medium in a suitable range, create good conditions for cell growth and metabolism, ensure the normal physiological function of cells, and prevent damage to cells due to pH fluctuations.
In molecular biology experiments, such as nucleic acid extraction and purification, the pH of the buffer has a significant impact on the structure and stability of nucleic acids. The stable alkaline environment created by CAPS can prevent nucleic acid degradation and ensure that the extracted nucleic acid remains intact, laying the foundation for subsequent gene cloning, PCR amplification and other experiments.

The preparation method of 3-Cyclohexylaminopropanesulfonic Acid is not detailed in the ancient book Tiangong Kaiwu, but it can be deduced according to similar methods and chemical principles.
First, use cyclohexylamine and 3-chloropropanesulfonic acid as raw materials, and add an appropriate amount of solvent, such as ethanol or acetone, in an appropriate reactor to facilitate full contact between the two. Control the reaction temperature, preferably between 50-70 ° C. This temperature can make the reaction proceed smoothly without frequent side reactions due to overheating. At the same time, the stirring rate should also be moderate to ensure that the reactants are mixed evenly. During the reaction, the amino group of cyclohexylamine undergoes a nucleophilic substitution reaction with the chlorine atom of 3-chloropropanesulfonic acid, and the chlorine atom leaves to form 3-cyclohexylaminopropanesulfonic acid and hydrogen chloride. In order to make the reaction go to the right completely, the amount of cyclohexylamine can be appropriately increased, and attention should be paid to tail gas treatment during the reaction process to remove the escaped hydrogen chloride.
Second, cyclohexylamine and propanesulfonic acid lactone can also be used as raw materials. The two are placed in the reaction vessel without additional solvent and react at a lower temperature, about 30-40 ° C. The ring structure of propanesulfonic acid lactone is active, and it is easy to undergo ring-opening addition reaction with the amino group of cycloh The procedure of this method is relatively simple and the purity of the product is high, but the price of acrylic sulfonate lactone is relatively expensive and the cost is slightly higher. After the reaction, the pure 3-cyclohexylaminopropane sulfonic acid can be obtained through separation and purification methods, such as recrystallization, column chromatography, etc.

3-Cyclohexylaminopropanesulfonic Acid is a commonly used buffer in biochemical research. When using it, many aspects need to be paid attention to.
The first to bear the brunt is its pH buffer range. The buffer range of this agent is about pH 9.3 - 10.7. When using, it is necessary to ensure that the pH value of the system to be adjusted falls within this effective range to achieve the ideal buffering effect. If it exceeds this range, its buffering capacity will be greatly reduced, and it is difficult to maintain the pH stability of the system.
Furthermore, the weighing must be accurate. Its dosage has a great impact on the pH and buffering effect of the buffer system. The weighing process requires the help of a high-precision balance, and an appropriate amount of reagents is weighed strictly according to the experimental requirements to avoid errors in experimental results due to deviations in quantity.
Dissolution should not be ignored. Generally speaking, it can be dissolved in an appropriate amount of pure water. When dissolving, it should be stirred or heated moderately (but the temperature should not be too high to prevent the denaturation of the reagents) to accelerate the dissolution process. At the same time, it is necessary to ensure complete dissolution and no undissolved particles, so as not to affect the follow-up experiments.
In addition, it is also necessary to pay attention to its compatibility with other reagents. In complex biochemical systems, when coexisting with many substances, chemical reactions may occur and interfere with Therefore, before use, it is necessary to understand its chemical properties in detail, anticipate the possibility of interaction with other components in the system, and avoid adverse reactions.
Furthermore, storage conditions are also critical. It should be stored in a dry and cool place to avoid moisture and heat. Due to changes in humidity and temperature or their properties, the shelf life will be shortened and the use effect will be affected.
In short, the use of 3-cyclohexylaminopropanesulfonic acid, from pH range control, accurate weighing, to dissolution operation, attention to compatibility and proper storage, all need to be treated strictly to ensure the smooth progress of the experiment and the reliability of the results.