3-Morpholino-2-Hydroxypropanesulfonic Acid

3 - Morpholino - 2 - Hydroxypropanesulfonic Acid, Chinese name 3 - morpholine - 2 - hydroxypropanesulfonic acid, often abbreviated as MOPSO, is a commonly used zwitterion buffer in biochemical research. Its main application fields are as follows:
First, in biochemistry and molecular biology experiments, MOPSO is widely used. Many enzymatic reactions are extremely sensitive to environmental pH, and MOPSO can create and maintain a stable pH environment to ensure that enzymes can exhibit optimal activity and stability. For example, in some nucleic acid-related experiments, such as RNA isolation and analysis, the MOPSO buffer system can effectively prevent RNA from being degraded due to pH fluctuations, ensuring the accuracy and reproducibility of experimental results.
Second, MOPSO is also common in the field of cell culture. Cell growth has strict requirements on the pH of the microenvironment, and MOPSO can adjust and maintain the pH stability of the cell culture medium, providing suitable conditions for cell growth. Whether it is adherent cells or suspended cell culture, MOPSO helps cells maintain normal morphology and function, and improves the success rate and quality of cell culture.
Third, in protein research, MOPSO buffer plays an important role. Protein purification, crystallization and properties research require specific pH conditions. MOPSO can precisely regulate the pH of the environment and help proteins maintain their natural conformation and biological activity, which is of great significance for in-depth exploration of the relationship between protein structure and function.
Fourth, in electrophoresis experiments, MOPSO is also indispensable. As an electrophoresis buffer component, MOPSO can maintain a stable pH environment during electrophoresis, ensuring that biological macromolecules (such as proteins, nucleic acids, etc.) can be effectively separated in the gel according to their own charge and size, so as to obtain a clear and accurate electrophoresis map, which lays the foundation for subsequent analysis.

3 - Morpholino - 2 - Hydroxypropanesulfonic Acid, the Chinese name is often called 3 - morpholino - 2 - hydroxypropanesulfonic acid, its physical and chemical properties are as follows:
This substance is a white crystalline powder with good solubility in water and can be dissolved in some organic solvents. Its melting point is in a specific range, which is helpful for identification and purification. In aqueous solution, it will exhibit buffering capacity and can effectively maintain the stability of the pH value of the solution within a specific pH range.
When it comes to chemical stability, it is quite stable under normal conditions. However, when exposed to strong oxidizing agents, strong acids or bases, or under extreme conditions such as high temperature and high humidity, chemical reactions may occur, causing changes in its structure and properties.
This substance has little impact on the environment and will not cause serious harm to the environment under normal use and disposal conditions. However, for safety reasons, proper environmental protection procedures should still be followed. For storage, it should be stored in a cool, dry and well-ventilated place away from incompatible substances to prevent deterioration or dangerous reactions. In short, knowing its physical and chemical properties is essential for the correct use, storage and rational application of this substance in related fields.

3 - Morpholino - 2 - Hydroxypropanesulfonic Acid, that is, 3 - morpholino - 2 - hydroxypropanesulfonic acid, often referred to as MOPSO, compared with similar products, it has many advantages.
First, the acid-base buffering ability is excellent. The pKa value of MOPSO is about 7.2 to 7.6, which is in the pH range commonly used in physiological and biochemical research, and the buffering efficiency is quite good. Compared with ordinary buffers, such as phosphoric acid buffer, MOPSO can more stably maintain a constant pH when dealing with acid-base changes in the system, ensuring a stable reaction or experimental environment. This is essential for pH-sensitive biochemical reactions.
Second, the chemical properties are stable. Under common experimental conditions, MOPSO is not easy to chemically react with other substances in the system, such as metal ions, proteins, nucleic acids, etc. On the other hand, some buffers containing active groups are easy to react with the components of the system and cause deviations in experimental results. MOPSO can avoid this situation and provide protection for the accuracy of experimental results.
Third, biocompatibility is excellent. In biological-related experiments such as cell culture and enzyme activity determination, MOPSO is non-toxic and interferes with cells and biological macromolecules. Compared with some buffers that affect cell growth or enzyme activity, MOPSO can create a more natural environment for cells and make the experimental results more suitable for the real situation in organisms.
Fourth, good solubility. Whether it is in water or some organic solvents, MOPSO has good solubility, which makes it convenient for experimenters to flexibly select suitable solvent systems for preparation according to different experimental needs and broaden its application range.

3 - Morpholino - 2 - Hydroxypropanesulfonic Acid, or 3 - morpholino - 2 - hydroxypropanesulfonic acid, is often abbreviated as MOPSO. The market price range varies depending on a number of factors.
In terms of raw material costs, if the price of the basic raw materials required for production fluctuates, the price of MOPSO will also be affected. If the starting materials for synthesizing the substance are abundant and inexpensive, the price of the resulting MOPSO may be relatively low; conversely, if the raw materials are scarce and difficult to obtain, the price will increase.
Product purity is the key factor. High-purity MOPSO, such as 99% purity and above, is often used in scientific research experiments, high-end biopharmaceuticals and other fields that are very sensitive to impurities, and its price is often higher, with a price of tens or even hundreds of yuan per gram. And products with slightly lower purity, such as 95% - 98% purity, may be used in general industrial production, and the price is relatively close to the people. The price per gram may range from a few yuan to more than ten yuan.
Packaging specifications also affect the price. Small packages, such as 1 gram, 5 grams and other packages suitable for small-scale trial by scientific research institutions, usually have higher unit prices; large packages, such as 500 grams, 1 kilogram or even larger, will reduce the unit price due to the elimination of multiple packaging costs.
Prices are also different in different sales channels. Purchased from well-known chemical reagent suppliers, the product quality is strictly controlled and the price is relatively high; while purchased from some small chemical raw material distributors, the price may be more competitive, but the product quality needs to be carefully screened.
Overall, the MOPSO market price range fluctuates greatly, from a few yuan per gram to hundreds of yuan. The specific price needs to be comprehensively judged in combination with the above factors.

3 - Morpholino - 2 - Hydroxypropanesulfonic Acid, Chinese name 3 - Morpholino ethanesulfonic acid, often abbreviated as MOPSO, its preparation process is of moderate complexity in the field of fine chemicals.
This compound is prepared by taking morpholine and epichlorohydrin, dissolving it in a suitable organic solvent, such as ethanol or dichloromethane. Under a certain temperature and catalyst action, the nucleophilic substitution reaction occurs between the two. This step requires precise control of the temperature and the ratio of the reactant. If the temperature is too high, the side reaction will increase and the yield will decrease. If the ratio is improper, the raw material will remain and the subsequent separation will be complicated. After the reaction is completed, the obtained product reacts with sodium sulfite under specific conditions to form the target product MOPSO. The reaction conditions in this step are also critical, such as reaction temperature, time, and the amount of sodium sulfite, which have a great impact on the purity and yield of the product.
After the reaction is over, it is not easy to separate and purify the product. Usually, the organic solvent is removed by vacuum distillation, and then further purified by suitable methods, such as recrystallization or column chromatography. During recrystallization, the appropriate solvent and crystallization conditions need to be selected, and column chromatography should consider the selection of stationary phase, mobile phase, and elution conditions, which will affect the quality of the final product.
From the perspective of raw material acquisition, morpholine, epichlorohydrin, and sodium sulfite are all common chemical raw materials and are easy to purchase. However, the production process needs to follow strict operating specifications and quality control standards to ensure stable product quality. In short, the preparation process of 3 - Morpholino - 2 - Hydroxypropanesulfonic Acid has a certain complexity, and each link is closely interlocked, which requires fine operation and strict control.