What is 3-Morpholino-2-Hydroxypropanesulfonic Acid Sodium Salt?
3 - Morpholino - 2 - Hydroxypropanesulfonic Acid Sodium Salt, 3 - morpholine - 2 - hydroxypropane sulfonate sodium salt, has a wide range of uses.
In the field of biochemistry and molecular biology, it is often used as a biological buffer. Because it can precisely maintain the pH stability of solutions within a specific pH range, it plays a key role in various biological experiments. For example, in the research of proteins and nucleic acids, the pH of the reaction system has a significant impact on the structure and function of biological macromolecules. This buffer can ensure the pH stability of the experimental environment, so that proteins and nucleic acids maintain their natural conformation and activity, thus ensuring the accuracy and reliability of experimental results.
Cell culture work is also inseparable from it. When cells are cultured in vitro, they are extremely sensitive to environmental pH. This substance can create a suitable pH environment and help cells grow and multiply healthily. Like common mammalian cell cultures, the precise pH range is of great significance for physiological processes such as cell adhesion and proliferation. Sodium 3-morpholine-2-hydroxypropane sulfonate provides cells with stable pH conditions.
In the study of enzymatic reactions, it is also indispensable. The activity of enzymes is closely related to the pH of the reaction system, and different enzymes often have their optimum pH value. This salt can effectively regulate the pH of the reaction environment, so that the enzyme is in the best active state, which is conducive to in-depth exploration of the catalytic mechanism and kinetic properties of the enzyme.
In the field of analytical chemistry, it also has important applications. In some analytical detection methods, maintaining the stability of solution pH is crucial to ensure the sensitivity and accuracy of detection. For example, when analyzing biological samples by high performance liquid chromatography, the pH of the mobile phase needs to be strictly controlled. Sodium 3-morpholine-2-hydroxypropanesulfonate can adjust and maintain the pH on demand to ensure the accuracy of analytical results.
What are the physical and chemical properties of 3-Morpholino-2-Hydroxypropanesulfonic Acid Sodium Salt
3 - Morpholino - 2 - Hydroxypropanesulfonic Acid Sodium Salt, that is, 3 - morpholino - 2 - hydroxypropane sulfonate sodium salt, its physicochemical properties are as follows:
This substance is in the state of white crystalline powder. Its solubility is quite good, and it is easily soluble in water. This characteristic makes it easy to disperse and dissolve in many aqueous systems in experimental and industrial applications.
In terms of pH, its aqueous solution can exhibit a specific pH range. In the field of biochemical research, this property is particularly critical. Because many biochemical reactions need to be carried out in a precise pH environment, it is often used to build a stable and appropriate buffer system to maintain the acid-base stability of the reaction environment and ensure the activity of biomolecules and the normal advancement of the reaction.
From the perspective of chemical stability, under conventional storage conditions, in a dry, cool and ventilated place, it can maintain a relatively stable state, and the chemical structure is not prone to significant changes. However, exposure to extreme conditions, such as high temperature, high humidity or strong acid-base environments, may affect its stability and cause changes in chemical properties.
Its melting point is also one of the important physical and chemical properties. The specific melting point makes it possible to control the temperature conditions according to this physical parameter during heating or melting operations, ensuring that the substance is in the desired physical state for subsequent processing and application.
These physical and chemical properties of this substance determine that it has a wide range of applications in scientific research fields such as biochemistry and molecular biology, as well as in some industrial production links, such as drug research and development, biological product production, etc.
3-Morpholino-2-Hydroxypropanesulfonic Acid Sodium Salt is widely used in which areas
3 - Morpholino - 2 - Hydroxypropanesulfonic Acid Sodium Salt, which is 3 - morpholino - 2 - hydroxypropane sulfonate sodium salt, has been widely used in many fields such as biochemistry, pharmaceutical research and development.
In the field of biochemical research, because of its good buffering properties, it can effectively maintain a specific pH environment, just like a stable "stage" for biochemical reactions, so it is highly relied on in the study of biological macromolecules such as proteins and enzymes. Proteins and enzymes are extremely sensitive to the pH of their environment, and slight deviations may affect their structure and function. This salt can ensure the pH stability of the reaction system, allowing researchers to accurately explore the characteristics and behavior of biomacromolecules in a suitable environment, just like creating a comfortable and stable "home" for them.
In the field of pharmaceutical research and development, drug stability and solubility are crucial in the process of drug development. This salt can adjust the pH of drug formulations, enhance drug stability, improve drug solubility, and help drugs work more efficiently. For example, in the development of injectables, it can ensure the stable existence of drugs in solution, avoid drug degradation or precipitation due to pH fluctuations, and act as an escort for drug transportation in the body, so that it can smoothly reach the target of action and exert its therapeutic effect.
Furthermore, its presence is also common in the field of cell culture. Cell growth requires strict environmental requirements, and suitable pH is one of the key factors. The salt can adjust the pH of the cell culture medium, create a suitable growth environment for cells, and ensure the normal metabolism and proliferation of cells. It is like creating an ideal "growth hotbed" for cells, helping researchers obtain high-quality cell samples, and promoting the process of cell biology research and biological product production.
3-Morpholino-2-Hydroxypropanesulfonic Acid Sodium Salt
3 - Morpholino - 2 - Hydroxypropanesulfonic Acid Sodium Salt, that is, 3 - morpholino - 2 - hydroxypropane sulfonate sodium salt, this material is suitable for storage in a cool, dry and well-ventilated place. Its nature may be afraid of moisture and heat, so it should be avoided in a humid place, and it should not be near a high temperature. In the storage place, the temperature should be kept in a moderate environment, and it should not be too high, so as not to cause changes in its properties or reduce its effectiveness. And it should be prevented from being stored with other substances that are worried about phasing, so as not to cause chemical changes. The reservoir should be tightly closed to prevent moisture and sundries from entering and damaging its quality. After use, it is quickly re-sealed to ensure that it is stored in good condition, so that it can be stored for a long time without losing its character for future needs.
What is the production method of 3-Morpholino-2-Hydroxypropanesulfonic Acid Sodium Salt?
3 - Morpholino - 2 - Hydroxypropanesulfonic Acid Sodium Salt, that is, 3 - morpholino - 2 - hydroxypropane sulfonate sodium salt, the preparation method often follows the following path.
The starting material is to take more morpholine and epoxy chloropropane. The two are combined, and 3 - morpholino - 2 - chloropropanol is obtained first. In this step, the reaction needs to be selected at a suitable temperature and pressure, and with the help of the catalyst, the reaction can be smooth.
Then, 3 - morpholino - 2 - chloropropanol meets sodium sulfite. After the reaction of nucleophilic substitution, the sodium salt of 3-morpholinyl-2-hydroxypropane sulfonate is finally obtained. This reaction also needs to be controlled by temperature and time to achieve the best yield.
In the preparation process, all conditions need to be carefully controlled. The temperature is related to the reaction rate and product purity; the length of time also affects the formation of the product. And the ratio of each reactant is also the key. Only when the proportion of raw materials is appropriate, the reaction can be sufficient, and the quantity and quality of the product can be complete.
After treatment, the art of separation and purification is often involved. The impurities can be removed by recrystallization, chromatographic separation, etc., to obtain pure 3-morpholino-2-hydroxypropane sulfonate sodium salt, which can meet the requirements of various applications.