3-Morpholinopropanesulfonic Acid

3-Morpholinopropanesulfonic Acid, or 3-morpholinopropanesulfonic acid, is often referred to as MOPS. This is a commonly used zwitterion buffer in biochemical research. Its main uses are as follows:
First, in biochemical and molecular biology experiments, it is often used as a key component of the buffer system. Many biochemical reactions are quite sensitive to environmental pH, and MOPS can effectively maintain the stability of the reaction system within a specific pH range, providing a suitable pH environment for enzymatic reactions, protein and nucleic acid research. For example, in RNA-related experiments, because it can accurately maintain the pH between 7.0 and 7.5, which meets the acid-base requirements of RNA stability, it is widely used in RNA electrophoresis buffers and other systems to prevent RNA from degrading due to pH discomfort and ensure the smooth development of the experiment.
Second, it is also common in the field of cell culture. Cell growth is extremely sensitive to the pH of the environment in which it is located. MOPS can help maintain the pH stability of the cell culture medium and create a good growth microenvironment for cells. In animal cell culture, plant protoplast culture and other scenes, MOPS is used to maintain a stable pH, which helps cells to metabolize and proliferate normally, and improves the success rate and quality of cell culture.
Third, in the study of protein crystallization, a suitable pH environment is of great significance for the formation of protein crystallization. The MOPS buffer system can finely adjust the pH of the solution and assist proteins to crystallize in a specific form, which is conducive to the subsequent analysis of the three-dimensional structure of proteins by X-ray crystallography and other techniques, and promotes the development of
Fourth, in some clinical diagnostic detection methods, MOPS is used to create a stable buffer environment to ensure stable performance of detection reagents and accurate and reliable detection results. For example, in some diagnostic methods based on enzyme-linked immunosorbent assay (ELISA), MOPS participates in maintaining the pH of the reaction system to ensure efficient and specific antigen-antibody reactions.

3-Morpholinopropanesulfonic Acid, Chinese translation of "3-morpholinpropanesulfonic acid", is a commonly used reagent in biochemical research. Its physical properties are as follows:
This agent is mostly white crystalline powder at room temperature, pure and delicate, smooth to the touch. The melting point is quite high, about 293-295 ° C. The high melting point indicates that the intermolecular force is strong and the structure is relatively stable.
The substance has very good solubility in water and can be miscible with water in various proportions. This property makes it very easy to disperse in water systems and easy to use in various aqueous phase experimental systems. Because it is an organic sulfonate, it has both hydrophilic sulfonic acid groups and nitrogen-containing heterocyclic structures, so it can form hydrogen bonds with water molecules and interact with them, thus being easily soluble in water. However, the solubility is low in common organic solvents such as ethanol and ether.
The density of 3-morpholine propane sulfonic acid is about 1.18 g/cm ³, and the density is moderate. This value reflects its mass per unit volume, which is of important reference significance for operations such as precise preparation of solutions and consideration of the proportion of materials in the reaction system.
In addition, its appearance is stable, and it is not easily deteriorated by factors such as air and light under conventional storage conditions. It provides convenience for experimental operation and long-term storage, so that related research can be carried out stably.

3 - Morpholinopropanesulfonic Acid (referred to as MOPS) is a commonly used buffer in biochemical experiments and has applications in many biochemical experimental scenarios.
First, in the field of enzyme activity research, it can create a stable pH environment and help enzymes maintain the best activity state. Gainase is extremely sensitive to the pH of the reaction environment, and small changes may affect its activity and structure. MOPS has excellent buffering ability and can effectively resist pH fluctuations caused by external factors, making enzyme experiment results more reliable.
Second, nucleic acid research is also indispensable. The extraction, purification and subsequent analysis of nucleic acids require specific pH conditions to ensure the stability and integrity of nucleic acid structure. MOPS can provide this suitable environment to prevent nucleic acid degradation or denaturation and ensure the smooth progress of the experiment.
Third, in the cell culture scene, it provides a stable pH microenvironment for cell growth. Cell growth has strict environmental requirements, and appropriate pH is the basis for normal cell metabolism and function. MOPS can help maintain the appropriate pH value of the culture medium to meet the needs of cell growth and improve the success rate of cell culture.
Fourth, in the protein electrophoresis experiment, as a key component of the buffer system, maintain pH stability during electrophoresis. In this way, proteins can be effectively separated in the gel according to their own charge and size, and a clear electrophoresis map can be obtained, which provides an important basis for protein analysis.
In summary, 3-Morpholinopropanesulfonic Acid plays an important role in many key links of biochemical experiments, and is of great significance to ensure the accuracy and reliability of experiments.

3 - Morpholinopropanesulfonic Acid is 3 - morpholine propanesulfonic acid. The following is the method of chemical synthesis for you to describe in detail:
Starting material, morpholine and 3 - chloropropanesulfonic acid can be selected. These two, under appropriate reaction conditions, can initiate nucleophilic substitution reaction.
Place an appropriate amount of morpholine carefully in the reaction vessel. Morpholine has nitrogen atoms, and the lone pair of electrons on its nitrogen is nucleophilic, and can be searched for electrophilic substrates to form a reaction.
Then, slowly add 3 - chloropropanesulfonic acid. The chlorine atom of 3 - chloropropanesulfonic acid is a good leaving group. In case of nucleophilic attack of morpholine, it can leave and connect the two.
The reaction needs to be carried out under an appropriate temperature environment. Usually, mild heating can increase the reaction rate, but the temperature should not be too high to prevent side reactions. If the temperature is too high, it may cause the decomposition of 3-chloropropanesulfonic acid, or initiate side reactions such as the polymerization of morpholine.
In order to make the reaction smooth, an appropriate amount of alkali can be added, such as potassium carbonate. The effect of alkali is to neutralize the hydrogen chloride generated by the reaction and promote the balance of the reaction to move in the direction of the product.
During the reaction process, it is advisable to continuously stir with a stirring device to allow the reactants to fully contact and accelerate the reaction process.
After the reaction is completed, the product needs to be separated and purified. The reaction mixture can be extracted with an organic solvent, mixed with an appropriate organic solvent, shaken and left to stand for stratification, so that the product is enriched in the organic phase. Then the organic solvent is removed by reduced pressure distillation to obtain a crude product.
The crude product still needs to be further refined and can be recrystallized. Select an appropriate solvent, dissolve the crude product, heat and concentrate it and cool it, so that the product crystallizes and precipitates, thereby obtaining a pure 3-Morpholinopropanesulfonic Acid.

3-Morpholinopropanesulfonic Acid, or 3-morpholinopropanesulfonic acid, often referred to as MOPS, has significant advantages over other similar compounds.
MOPS has good buffering properties, and can effectively maintain the pH stability of the solution within a specific pH range (about 6.5-7.9). Just like the good generals of ancient times, it can defend the city and keep the people safe in turbulent situations. In biochemical and molecular biology experiments, the activity and structure of many biological macromolecules are highly dependent on a stable pH environment. MOPS is like a mainstay, ensuring the pH stability of the experimental system and enabling biological macromolecules to function normally.
MOPS is chemically stable and does not easily react with common chemical reagents. Like a stable gentleman, does not follow the flow, sticks to its own character. In complex biochemical reaction systems, many reactants and products coexist. MOPS can maintain its own stability and do not interfere with the reaction process of other substances, laying the foundation for the smooth development of experiments.
MOPS has low toxicity to biological systems, just like a gentle healer, treating diseases and saving people without damaging the body. In experiments involving biological activity such as cell culture, the use of MOPS as a buffer can not only adjust pH, but also will not cause obvious adverse effects on cell growth and metabolism, which greatly guarantees the accuracy and reliability of biological experiments.
MOPS has good solubility. Whether it is an aqueous phase system or some organic phase systems, it can dissolve quickly and disperse evenly. It is like smart water, which can penetrate everywhere. This property makes it convenient to use in different types of experiments and reaction systems, without the need to worry about its dissolution problems.
In summary, 3-Morpholinopropanesulfonic Acid (MOPS) has advantages in biochemistry, molecular biology and other fields due to its stable buffering performance, chemical stability, low toxicity and good solubility. It has become a powerful choice for researchers.