3-(N-Ethyl-3-Methylanilino)-2-Hydroxypropanesulfonic Acid Sodium Salt

3 - (N - ethyl - 3 - methylaniline) - 2 - hydroxy propanesulfonate sodium salt, its chemical structure is as follows:
The main chain of this compound is the sodium propanesulfonate structure, that is, one end of propane is connected to the sulfonic acid group ($- SO_3Na $) to form the sodium sulfonate structure. Introducing a hydroxyl group ($-OH $) at the 2 - position of propane constitutes the basic skeleton of 2 - hydroxy propanesulfonate.
At the 3 - position, a substituted aniline group is connected by a nitrogen atom. On the aniline group, the nitrogen atom is connected to ethyl ($- CH_2CH_3 $), and the 3-position of the benzene ring is connected to methyl ($- CH_3 $), forming an N-ethyl-3-methylaniline group structure. The overall structure can be abbreviated as: The hydrogen atom of $CH_2 $in $CH_3CH (OH) CH_2SO_3Na $is replaced by $N $-ethyl-3-methylaniline.
Its chemical structure shows the characteristics of both hydrophilicity (sodium sulfonate group and hydroxyl group) and certain hydrophobicity (substituted aniline group containing benzene ring, ethyl group and methyl group). This structure endows the compound with unique properties and applications in specific fields, such as biological buffers.

3- (N-ethyl-3-methylaniline) - 2-hydroxypropanesulfonate sodium salt, which is commonly used in biochemical reagents. Its main application fields are quite wide, let me tell them one by one.
In biochemistry and molecular biology experiments, this substance is often used as a buffer. Because of its specific acid-base balance adjustment ability, it can create a stable acid-base environment and help maintain the structure and activity of biological macromolecules. Many enzymatic reactions are extremely sensitive to pH. In such reaction systems, this salt can ensure that the reaction is carried out efficiently within a suitable pH range, preventing enzyme inactivation due to pH fluctuations, which in turn affects the accuracy of experimental results.
is also indispensable in protein research. In the process of protein isolation, purification and analysis, the conditions of the solution need to be precisely controlled. As a buffer component, this salt can avoid protein denaturation due to environmental changes and maintain its natural conformation, allowing researchers to obtain more realistic and reliable protein characteristic data, laying the foundation for in-depth exploration of protein functions and mechanisms.
Furthermore, in the field of cell culture, cell growth requires strict requirements on the microenvironment in which it is located. This substance can adjust the pH value of the medium, creating an acid-base environment similar to that in the body for cells to ensure normal cell growth, proliferation and metabolism. If the acid-base imbalance of the medium, the physiological function of the cell will be seriously affected, and even apoptosis. Therefore, this salt is of great significance for maintaining the stability of the cell culture system.
In the process of drug development, the stability and solubility of drug molecules are key factors to consider. This salt can improve the bioavailability of drugs by adjusting the environmental pH, optimizing the solubility of drug molecules. At the same time, in the stability study of drug preparations, as a buffer component, it helps to evaluate the stability of drugs under different conditions, providing an important basis for the design and optimization of drug dosage forms.
In summary, sodium 3- (N-ethyl-3-methylanilino) -2-hydroxypropane sulfonate plays a pivotal role in many fields such as biochemistry, molecular biology, cell culture and drug development, and has made significant contributions to scientific research and medical development.

3 - (N - ethyl - 3 - methylaniline) - 2 - hydroxypropanesulfonate sodium salt should pay attention to the following matters during use.
First of all, this chemical has specific chemical properties and should strictly follow the established use procedures. When taking it, be sure to weigh it accurately to avoid inaccurate experimental or production results due to dosage deviations. If the dosage is too much, or the reaction is overdone, resulting in increased impurities in the product; if the dosage is too small, the reaction may not achieve the desired effect.
Furthermore, the substance may have an impact on the environment. After use, its waste should be properly disposed of and not discarded at will. It needs to be sorted and collected according to relevant environmental protection regulations to avoid pollution to the surrounding environment and avoid the harm of nature.
At the same time, because it may have certain chemical activity, storage conditions are also very critical. It should be stored in a dry, cool and well-ventilated place, away from fire and heat sources, to prevent deterioration or safety accidents caused by temperature and humidity discomfort or exposure to open flames.
In addition, during use, operators need to take protective measures. Appropriate protective gloves, goggles and masks should be worn to prevent the substance from coming into contact with the skin, eyes or being inhaled into the body and harming their own health.
When using 3- (N-ethyl-3-methylanilinyl) -2-hydroxypropane sulfonate sodium salt, care should be taken in terms of dosage, waste disposal, storage conditions, and personnel protection, so as to ensure the safety and effectiveness of use.

Sodium salt of 3- (N-ethyl-3-methylanilinyl) -2-hydroxypropanesulfonate, the physical properties of this substance are particularly important, and it is relevant to its application in various fields.
Looking at its morphology, it is mostly white crystalline powder under normal conditions, with fine and uniform particles. This morphology gives it good fluidity and dispersibility. In the preparation of various preparations, it can be evenly dispersed smoothly to ensure the stability of the system.
When it comes to solubility, it shows good solubility in water and can dissolve quickly to form a clear and transparent solution. This property makes it easy to blend with other components and participate in various chemical processes in the reaction or formulation preparation of aqueous systems. In organic solvents, such as ethanol and acetone, its solubility is relatively limited, only slightly soluble or insoluble.
Melting point is also one of the key physical properties. Its melting point is in a specific temperature range, and this value is stable and measurable, providing an important reference for its heating processing or reaction conditions. When the temperature approaches the melting point, the substance will undergo a physical state transformation, gradually melting from a solid state to a liquid state. This property needs to be carefully considered when material processing or specific reaction environment settings.
In terms of density, it has a certain value, which determines its settlement or suspension characteristics in the mixed system. When blending with other substances, its distribution can be anticipated according to the density difference, which is of great significance for product performance optimization and process design.
In addition, its hygroscopicity should not be underestimated. In high humidity environments, this substance has a tendency to absorb moisture in the air. Moderate hygroscopicity can sometimes improve its interaction with surrounding media in some application scenarios, but excessive hygroscopicity may lead to undesirable phenomena such as caking, which affects its storage and use performance. In summary, the morphology, solubility, melting point, density and hygroscopicity of sodium 3- (N-ethyl-3-methylanilinyl) -2-hydroxypropanesulfonate play an indispensable role in the research, production and application of chemical, pharmaceutical, materials and many other fields, providing important guidelines for relevant practitioners to control this substance and exert its effectiveness.

3- (N-ethyl-3-methylanilinyl) -2-hydroxypropanesulfonate sodium salt, often used as a biological buffer. The preparation method is as follows:
The starting materials are N-ethyl-3-methylaniline and epoxy chloropropane. The two are heated to 50-80 ° C in a suitable solvent such as toluene or xylene with an appropriate amount of organic base such as triethylamine as catalyst for several hours to generate 3- (N-ethyl-3-methylanilinyl) -1-chloro-2-propanol. In this step, the epoxy ring of epoxy chloropropane is opened and nucleophilic substitution occurs with the amine group.
Mix 3- (N-ethyl-3-methylanilinyl) -1-chloro-2-propanol with an aqueous solution of sodium sulfite and react at 80-100 ° C for several hours. The chlorine atom is replaced by sulfite to form a sulfonate.
After the reaction is completed, the temperature is lowered, the solution is decolorized with activated carbon, and the activated carbon and insoluble matter are filtered off. The filtrate is evaporated and concentrated under reduced pressure, cooled and crystallized, and centrifuged to obtain a crude product.
The crude product is recrystallized with an appropriate amount of ethanol or ethanol-water mixed solvent to obtain a high-purity 3- (N-ethyl-3-methylanilinyl) -2-hydroxypropanesulfonate sodium salt. This preparation process has mild conditions, good conversion and product purity at each step, and can meet the needs of industrial and laboratory preparation.