3-(N-Morpholine)Propane Sulphonic Acid,Sodium

Sodium 3- (N-morpholino) propanesulfonate is a very important chemical substance. It is mild in nature and often acts as a buffer in many chemical and biological systems.
In terms of its chemical properties, this substance has good water solubility. Due to its strong hydrophilicity of the sulfonic acid group in its structure, it is easy to interact with water molecules, so it can quickly dissolve in water to form a uniform solution. And within a certain pH range, it can maintain the relative stability of the pH value of the solution.
In addition, its chemical stability is also good, and it is not easy to decompose or other chemical reactions under conventional temperature and environmental conditions. However, in the case of strong acids and bases, its structure may be affected, resulting in changes in buffering performance.
In its molecular structure, the morpholine ring gives it a specific alkaline characteristic, while the propanesulfonate group provides an acidic check point. The synergy between the two makes the substance exhibit excellent buffering ability in a specific pH range. With this characteristic, sodium 3- (N-morpholino) propanesulfonate is widely used in biochemical experiments, drug development and some industrial production processes to ensure that the pH conditions of the system meet the relevant needs.

Sodium 3- (N-morpholine) propanesulfonate, its common uses are as follows:
In the process of biochemical research, this is a commonly used biological buffer. Because it can maintain a stable pH in a specific pH range, it is like an anchor for a stable boat. In many biochemical experiments, such as enzyme activity determination, protein crystallization, etc., it creates a suitable and stable acid-base environment for the reaction system to avoid damage to the structure and function of biomolecules due to large fluctuations in pH.
In the way of cell culture, it can provide stable pH conditions for cell survival. Cell growth is extremely sensitive to environmental pH, and growth will be hindered if it is not careful. This substance precisely regulates pH, enabling cells to thrive in a suitable environment and ensuring normal metabolism and physiological functions of cells.
In the field of electrophoresis, as a key component of the buffer system, it ensures that the pH is constant during the electrophoresis process. In this way, charged biological macromolecules such as proteins, nucleic acids, etc. swim in an orderly manner according to their own charge and molecular size in the electric field to achieve efficient separation and accurate analysis.
In the process of drug development, it is indispensable in the research of drug preparations. It can adjust the pH of drug solutions, enhance the stability and solubility of drugs, and act like a clever craftsman to optimize the quality of drugs, laying the foundation for the normal efficacy of drugs. It can also be used to simulate the physiological environment, evaluate the behavior of drugs in the body, and help researchers gain insight into drug performance and promote the research and development process

3- (N-morpholine) propanesulfonic acid (MOPS) sodium salt has a wide range of uses in biological experiments. First, it can provide a stable pH environment for biological systems. Biochemical reactions are mostly pH-sensitive, and MOPS sodium salt can resist large fluctuations in pH and ensure the stability of the reaction system due to its buffering ability. For example, in enzymatic reactions, specific enzymes need to be suitable for pH activity, and MOPS sodium salt buffer can create and maintain this condition to help enzymes with high-efficiency catalysis. Second, it is used for protein and nucleic acid research. During protein purification and analysis, MOPS sodium salt buffer can maintain the stability of protein structure and function, and prevent protein denaturation. In nucleic acid electrophoresis experiments, it can provide a stable ionic environment, so that nucleic acids can be effectively separated according to their own charge and size, helping researchers to obtain clear electrophoresis bands and analyze nucleic acid properties. Third, cell culture is also commonly used. Cell growth has strict environmental requirements, and MOPS sodium salt buffer can regulate the pH of the medium, protect the growth and reproduction of cells in suitable acid-base conditions, promote healthy metabolism of cells, and lay the foundation for the smooth development of cell experiments. In short, 3- (N-morpholine) propanesulfonate sodium salt is a key reagent for ensuring the stability and accuracy of experiments in all fields of biological experiments.

The sodium salt of 3- (N-morpholinyl) propanesulfonic acid (MOPS) needs to be properly stored to ensure its stability and use it correctly. According to the rules of "Tiangong Kaiwu", the material storage method is related to physical properties and the environment. This agent should be placed in a cool and dry place away from direct sunlight. Sunlight can promote its chemical modification and damage its purity and activity.
In addition, the humidity of the air is also the key. Moisture can easily dehydrate this salt, causing its morphology and properties to change. Therefore, it should be sealed and stored in a dryer to prevent moisture from invading. Furthermore, the temperature should not be ignored. Overheating will cause molecular movement or decomposition reactions; under certain conditions, it may cause crystal transformation and other conditions. Room temperature (about 15-25 degrees Celsius) is appropriate to maintain its chemical structure and properties.
In addition, the storage place should avoid coexistence with strong oxidizing agents, reducing agents and substances with great acidity and alkalinity. Because of its chemical properties, or react with various substances to cause deterioration. In this way, a proper method of storing 3 - (N - morpholino) propanesulfonate sodium salt can be used in experiments or production to obtain reliable results.

To prepare sodium 3- (N-morpholine) propanesulfonate, the method is as follows:
First, prepare the required raw materials. N-morpholine, sodium 3-chloropropanesulfonate, etc. are required. N-morpholine is the key amine reagent for the reaction, and sodium 3-chloropropanesulfonate is the raw material for providing sulfopropyl.
Place an appropriate amount of N-morpholine in a reaction vessel and dissolve it in an organic solvent, commonly used as ethanol, acetone, etc., which can disperse the reactants uniformly and facilitate the reaction. Stir to fully disperse N-morpholine in the solvent.
Then, slowly add sodium 3-chloropropanesulfonate. This process needs to control the dripping speed to prevent the reaction from being too violent. After adding, heat up to a suitable temperature, about 50-80 ° C. When heated, the molecular movement in the reaction system intensifies, and the nucleophilic substitution reaction occurs between the two. The nitrogen atom in N-morpholine is rich in electrons, attacking the carbon atom connected to the chlorine atom in sodium 3-chloropropanesulfonate, and the chlorine leaves the atom to form 3- (N-morpholine) sodium propanesulfonate.
During the reaction process, the reaction progress can be monitored by thin layer chromatography (TLC) or high performance liquid chromatography (HPLC). When the raw material point is basically eliminated, the reaction is complete.
After the reaction is completed, the reaction solution is cooled to room temperature. Then, the organic solvent is removed by distillation under reduced pressure. After that, add water to dissolve the residue, and then extract impurities with an appropriate amount of organic solvent such as ether. Take the aqueous phase and adjust the pH to neutral with an appropriate amount of acid or base.
Finally, concentrate and crystallize the aqueous phase. The method of evaporation concentration and cooling crystallization can be used. After the crystals are precipitated, suction filtration, wash the crystals with a small amount of cold water, remove surface impurities, and dry in vacuum to obtain a pure 3- (N-morpholine) sodium propanesulfonate product. The whole preparation process should pay attention to the control of reaction conditions and the operation specifications of each step to obtain a high-purity product.