3- (N-Morpholine) Propanesulfonic Acid Half Sodium Salt Mops Half Sodium Salt

3- (N-morpholine) propanesulfonate sodium salt hemisodium salt (MOPS hemisodium salt), its main use is quite extensive. In the field of biochemistry and molecular biology experiments, this is a commonly used buffer. Because it can effectively maintain the stability of a specific pH range, it plays a key role in many biochemical reactions and enzymatic reactions. Many enzymes are extremely sensitive to environmental pH, and MOPS hemisodium salt can create a suitable reaction environment to ensure enzyme activity and smooth reaction.
In the field of cell culture, it is also indispensable. Cell growth has strict requirements on the pH of the environment. MOPS hemisodium salt can adjust the pH value of the culture medium, create a stable living environment for cells, promote normal cell growth and proliferation, and prevent damage to cells due to pH fluctuations.
In protein research, its buffering properties are helpful for the separation, purification and identification of proteins. In protein analysis technologies such as electrophoresis and chromatography, stabilizing pH conditions is of great significance for accurate analysis of protein characteristics, and MOPS hemisodium salt can provide such stable conditions.
In clinical diagnostic reagents, it is also often used as a buffer system component. The reaction of diagnostic reagents needs to be carried out accurately in a specific pH environment. MOPS hemisodium salt can ensure the stability of reagent performance, improve the accuracy and reliability of test results, and provide strong support for disease diagnosis.

Sodium 3- (N-morpholine) propanesulfonate (MOPS hemisodium salt) is a commonly used biochemical reagent, which is often used as a buffer in biochemical experiments. The pH buffer range of this substance is about 6.5 to 7.9.
The reason why MOPS hemisodium salt has such a buffer range is that there are specific acid-base groups in its chemical structure. In this pH range, these groups can effectively maintain the relative stability of the pH value of the solution by means of proton gains and losses. When external factors cause the solution to become more acidic, that is, when hydrogen ions increase, the basic groups in this buffer will bind hydrogen ions, thereby alleviating the decrease in pH value; conversely, if the solution becomes more alkaline and hydrogen ions decrease, the acidic groups will release hydrogen ions to inhibit the increase in pH value.
In many biochemical experiments, such as enzyme activity determination, protein electrophoresis, etc., the pH value of the reaction system needs to be precisely controlled within a specific range, and MOPS semi-sodium salts, because of their suitable buffering range, can conform to the near-neutral environment required for chemical reactions in many organisms, so they are widely used in such experiments to ensure the accuracy and reliability of experimental results.

3- (N-morpholine) propanesulfonate hemisodium salt (MOPS hemisodium salt) has different compatibility in different experimental systems. In biochemical experimental systems, this substance is often used as a buffer because it can effectively maintain the stability of the pH of the system. It has good compatibility with many biomacromolecules, such as proteins and nucleic acids, and rarely interferes with the structure and function of these biomolecules. This makes it widely used in experiments such as enzyme activity determination, protein purification and analysis, providing a stable acid-base environment for experiments and ensuring that biomolecular properties are not affected.
In cell culture systems, the compatibility of MOPS hemisodium salt is also a key consideration. It can adjust the pH value of the cell culture medium to a certain extent and create a suitable environment for cell growth. However, its concentration needs to be carefully regulated. Excessive concentration may be toxic to cells and affect cell growth, metabolism and differentiation. Therefore, it is necessary to accurately determine the optimal use concentration according to the cell type and culture conditions to ensure the normal physiological function of cells.
In the chemical synthesis system, the compatibility of MOPS semi-sodium salt depends on the type of chemical reaction involved. In some organic synthesis reactions sensitive to pH, it can be used as a buffer to maintain the appropriate pH value of the reaction system, and has good compatibility with many organic reagents without hindering the reaction process. However, in specific metal-catalyzed reactions or reactions that are extremely sensitive to impurities, the potential impact on the reaction needs to be carefully evaluated, as the presence of sodium ions or other impurities may interfere with catalyst activity and affect reaction selectivity and yield.

3 - (N -morpholine) propanesulfonate semi-sodium salt (MOPS semi-sodium salt) should be stored in a cool, dry and well-ventilated place. This product is afraid of moisture and moisture, and moisture can easily damage its properties. Therefore, it needs to be placed in a closed container to prevent moisture from invading. Temperature is also critical, and it should be controlled between 15 and 25 degrees Celsius. If it is too high, it may cause it to decompose and deteriorate. Although it is too low, it will not damage its quality temporarily, but it is inconvenient to use and may affect the follow-up experimental results.
Store away from fire and heat sources, and avoid mixing with oxidants, acids, alkalis, etc., to prevent chemical reactions. After taking it, the container must be sealed in time and returned to the original place to keep its environment as it was before. In this way, the quality of 3 - (N - morpholine) propane sulfonate semi-sodium salt can be maintained for a long time to prepare for the needs of scientific research and experiments without losing its effectiveness.

3- (N - Morpholine) Propanesulfonic Acid Half Sodium Salt (MOPS Half Sodium Salt) is a commonly used buffer in biochemical experiments. The correct way to prepare and use it is as follows:
First, you need to prepare all the utensils and materials used, such as balances, volumetric bottles, glass rods, distilled water, etc. According to the required concentration and volume of the experiment, the required mass of MOPS Half Sodium Salt can be accurately calculated. If you want to prepare a solution of a specific concentration, assume that you need to prepare a solution of 0.1M 1L, and calculate the required solute mass according to its molar mass.
Weigh the MOPS Half Sodium Salt accurately with a balance and put it in a beaker with an appropriate amount of distilled water. Stir slowly with a glass rod to help it dissolve. If it is difficult to dissolve, it can be heated moderately, but the temperature should not be too high to prevent the solute from deteriorating.
After the solute is completely dissolved, transfer the solution to the corresponding volume of volumetric flask. Rinse the beaker and glass rod several times with a small amount of distilled water, and pour the rinsing liquid into the volumetric flask to ensure the complete transfer of the solute.
Then, add distilled water to the vicinity of the scale line of the volumetric flask. Use a dropper to add it dropwise until the concave liquid level of the solution is tangent to the scale line. Cover the cork tightly and shake it upside down to make the solution uniform.
When using, pay attention to its applicable pH range. The buffer range of MOPS Half Sodium Salt is about pH 6.5 - 7.9. It should be reasonably selected according to the pH requirements of And the prepared solution should be properly stored, generally placed in a cool, dry place to prevent contamination and deterioration. In this way, it is necessary to accurately prepare and properly use MOPS Half Sodium Salt to provide guarantee for the smooth development of the experiment.