3-(N-Morpholino)-2-Hydrixypropanesulfonic Acid

3- (N-morpholino) -2-hydroxypropanesulfonic acid, which is a commonly used zwitterion buffer in the field of biochemistry. It has the following chemical properties:
- ** Properties **: Under normal conditions, it is mostly white crystalline powder, like fine snow, pure in quality and clean in appearance. This characteristic makes it easy to accurately weigh and use during experimental operation.
- ** Solubility **: It is very soluble in water, just like snowflakes blending into streams, and can quickly dissolve with water to form a uniform and transparent solution. However, in common organic solvents, such as ethanol, ether, etc., the solubility is quite low, only slightly soluble or almost insoluble. This solubility difference can be exploited in the process of separation and purification.
- ** Buffering performance **: In a specific pH range, usually between pH 6.5 and 7.9, it exhibits excellent buffering ability. Like a rock-solid dam, it can effectively resist the violent fluctuations in pH caused by the addition of a small amount of acid or alkali, and maintain the relative stability of the pH value of the solution. This property is of great significance in many biochemical reactions and biological experiments. Because the structure and function of many biomolecules are extremely sensitive to environmental pH, slight changes may affect the accuracy and reliability of the experimental results.
- ** Chemical stability **: Under conventional storage and experimental conditions, it has good chemical stability, and the molecular structure is not easy to change spontaneously. It is like a strong fortress and is not easily eroded by external factors. However, under extreme conditions such as strong acid, strong alkali or high temperature, the structure may be damaged, and the buffering performance will also be damaged.
- ** Dissociation characteristics **: Its molecular structure contains both acidic and basic groups, which can be dissociated according to the pH environment in solution. When the pH is lower than its pKa value, some groups bind protons in a protonated state; when the pH is higher than the pKa value, the groups release protons in a dissociated state. This dissociation property is the fundamental reason for its buffering effect. By absorbing and releasing protons, the pH of the solution is cleverly maintained.

3- (N-morpholino) -2-hydroxypropanesulfonic acid is often used as a biological buffer in many fields.
In biochemistry and molecular biology, this agent can stabilize enzyme, protein structure and activity. Many enzymatic reactions are pH-sensitive and require a specific pH range to exert the best activity. Like some proteases, they are highly active in near-neutral pH environments. 3- (N-morpholino) -2-hydroxypropanesulfonic acid can adjust the pH in the reaction system to a suitable range, ensuring efficient catalysis of proteases, allowing researchers to accurately measure enzyme kinetic parameters and substrate specificity.
In the field of cell culture, it is also critical. Cell growth requires harsh environmental pH, and small changes can affect cell metabolism, proliferation and differentiation. Using this buffer can maintain the pH stability of cell culture medium, create a suitable microenvironment for cells, and help cells grow and pass on normally. For example, in culturing mammalian cells, using the buffer medium can avoid cell damage or death caused by pH fluctuations, and improve the success rate and quality of cell culture.
In drug development, especially in vitro activity evaluation of drugs, 3- (N-morpholino) -2-hydroxypropanesulfonic acid is widely used. Drug development often needs to simulate the human physiological environment, which can help adjust the pH of the reaction system to approach the human physiological pH, and accurately evaluate drug activity, stability and interaction with targets. Taking the development of anti-cancer drugs as an example, in vitro experiments, using this buffer to create a suitable pH environment can better observe the inhibitory or killing effect of drugs on cancer cell growth, providing a reliable basis for drug screening and optimization.

3- (N-morpholine) -2-hydroxypropanesulfonic acid, or MOPS, is widely used in biological experiments.
Its primary use is as a buffer. Biochemical reactions in organisms require a specific pH environment to proceed smoothly. MOPS has a good buffering ability, which can maintain the pH stability of the reaction system and avoid affecting the structure and function of biomolecules due to pH fluctuations. Such as enzymatic reactions, the activity of enzymes is often extremely sensitive to pH. MOPS can create suitable pH conditions to ensure the efficient occurrence of enzymatic reactions.
Furthermore, in the field of cell culture, MOPS is also indispensable. Cell growth requires strict environmental requirements, and appropriate pH is the basis for normal cell growth and metabolism. MOPS can adjust the pH of cell culture medium, create a stable microenvironment for cells, help cells grow and proliferate, and maintain normal physiological functions of cells.
In addition, in nucleic acid and protein research, the role of MOPS should not be underestimated. During nucleic acid extraction, purification and electrophoresis analysis, MOPS buffer can prevent nucleic acid degradation, ensure its structural integrity, and facilitate subsequent analysis. Protein-related experiments, such as protein electrophoresis, Western blot, etc., MOPS can stabilize protein charge and conformation, making protein separation and detection results more accurate.
In summary, 3- (N-morpholino) -2-hydroxypropanesulfonic acid plays a key role in many aspects of biological experiments, which is of great significance for ensuring the smooth development of experiments and obtaining reliable results.

3- (N - Morpholino) -2 - Hydroxypropanesulfonic Acid (referred to as MOPSO) is a commonly used buffer in biochemical research. Its storage conditions are crucial to its quality and utility.
This substance should be stored in a cool, dry and well-ventilated place. If the temperature is too high, it is easy to change its chemical properties. If it is heated or initiates reactions such as decomposition, its buffering performance will be damaged. Therefore, it should be avoided to be stored in a high temperature environment. Generally speaking, the storage temperature is 2-8 ° C. In this temperature range, its stability can be effectively maintained.
Furthermore, humidity also has a great impact. Humid environment is easy to make MOPSO absorb moisture, after moisture absorption or agglomeration, or even deterioration, so it needs to be kept dry. When storing, it should be placed in a sealed container to prevent the intrusion of water vapor in the air.
In addition, it should be kept away from fire sources, heat sources and strong oxidants. Because it encounters open flames, hot topics or contact with strong oxidants, or there is a risk of combustion or explosion, it threatens the safety of the experiment.
In summary, 3- (N - Morpholino) -2 - Hydroxypropanesulfonic Acid should be stored in a cool and dry place at 2-8 ° C, in a sealed container, and away from fire sources, heat sources and strong oxidants, so as to ensure its stable performance during use and meet the needs of biochemical research.

"3- (N - Morpholino) -2 - Hydroxypropanesulfonic Acid" is 3- (N-Morpholino) -2-hydroxypropane sulfonic acid, often abbreviated as MOPSO, which is a biological buffer. The price range varies greatly depending on the purity, packaging specifications, brands and purchase channels.
In the general biochemical reagent procurement platform, taking the common analytical purity grade and 25g package as an example, the price is roughly 50 to 150 yuan. If it is higher purity, such as for cell culture grade, the same 25g package, the price may climb to 200 to 400 yuan.
The purchase volume also has a significant impact on the price. If you purchase 100 grams of analytical pure products, the total price may be 150 to 400 yuan; if it reaches 500 grams, the unit price per gram will be further reduced, and the total price will be about 500 to 1,500 yuan.
Different brands have different prices due to different R & D investment, production process and market positioning. Well-known brands pay attention to quality control and research and development, product quality is stable, and the price is relatively high; some emerging brands or focus on cost performance, the price will be slightly lower.
Online platform promotions, offline dealer volume discounts, etc., will also cause the actual purchase price to fluctuate. When purchasing, you need to comprehensively consider your own needs, budget and product quality, and compare through multiple channels to get a suitable price.