3-(N-Morpholino)-2-Hydroxy-1-Propanesulfonic Acid

3- (N-morpholine) -2-hydroxy-1-propanesulfonic acid, often referred to as MOPSO, has a wide range of uses and is important in the field of biochemical research.
First, in the biological buffer system, MOPSO has a significant effect. Chemical reactions in organisms need to be carried out in a specific pH environment. MOPSO can maintain the pH stability of the reaction system because of its good buffering ability. If many enzymatic reactions are extremely sensitive to pH, MOPSO can effectively avoid pH fluctuations affecting enzyme activity and ensure the normal progress of the reaction. In protein crystallization experiments, precise control of pH is crucial. MOPSO can create a suitable pH environment, help protein crystallization formation, and facilitate researchers to analyze protein structures with the help of X-ray crystallography and other technologies, laying the foundation for in-depth exploration of protein functions and mechanisms of action.
Second, in the field of cell culture, MOPSO is also indispensable. Cell growth requires strict environmental requirements, and a suitable pH environment is the key to normal cell metabolism and proliferation. MOPSO is used in cell culture media to stabilize the pH value of the system and create a stable living environment for cells. Like mammalian cell culture, the pH needs to be maintained between 7.2 and 7.4. MOPSO plays an important buffer role in this process to ensure the smooth progress of cell culture, which is helpful for cell biology related research, such as cell proliferation, differentiation and signal transduction.
Third, in electrophoresis buffer, MOPSO plays an important role. Electrophoresis is a common technique for separating and analyzing biological macromolecules, and the pH stability of the buffer has a significant impact on the separation effect. MOPSO is used in electrophoresis buffer to maintain a stable pH environment, ensure the stable migration rate of biological macromolecules in the electric field, and achieve efficient separation. In nucleic acid electrophoresis, DNA or RNA fragments of different sizes can be well separated according to the difference in charge and size, which is convenient for subsequent research work such as nucleic acid testing and gene analysis.

3- (N-morpholino) -2-hydroxy-1-propanesulfonic acid, often referred to as MOPSO, is widely used in many fields such as biochemistry, medicine and molecular biology.
In the field of biochemical research, because of its good buffering performance, it can maintain the stability of the pH value of the system, like a solid barrier, guarding the smooth progress of biochemical reactions. Many enzymatic reactions are extremely sensitive to pH. MOPSO can precisely regulate the pH range to ensure that enzymes are in the best active state, just like setting a comfortable stage for enzymes to work.
In the process of pharmaceutical research and development, MOPSO also plays a key role. In pharmaceutical preparations, it can adjust pH, improve drug stability and solubility, and is like a guardian who carefully cares for drugs. For example, after the pH of some insoluble drugs is adjusted by MOPSO, the solubility is significantly improved, making it easier for the human body to absorb.
In molecular biology experiments, MOPSO is indispensable. For example, nucleic acid electrophoresis experiments require a specific pH environment to ensure the normal migration and separation of nucleic acid molecules. The stable pH conditions created by MOPSO make nucleic acid bands clearly present, providing researchers with the key to accurately interpret the genetic code.
In the field of cell culture, MOPSO also shows its talents. Cell growth requires strict environmental pH, and MOPSO can create a suitable microenvironment to help cells grow and multiply healthily, as if creating a comfortable home for cells. In conclusion, 3- (N-morpholino) -2-hydroxy-1-propane sulfonic acid, with its unique properties, plays an irreplaceable role in many important fields and contributes to scientific research and industrial development.

3- (N-morpholino) -2-hydroxy-1-propanesulfonic acid, this substance is in the state of white crystalline powder. Its solubility is quite good, easily soluble in water, and can form a clear solution. However, in most organic solvents, the solubility is poor.
When it comes to pH, this substance has good buffering properties, and its buffering range is about 6.5-7.9. In this range, it can effectively maintain the stability of the pH of the solution, so that the pH value does not change easily.
In terms of thermal stability, under the general experimental temperature conditions, the properties are quite stable, and it can withstand a certain degree of heating without decomposing. However, when the temperature is too high, it will also affect its structure and properties.
Chemically, because its structure contains active groups such as hydroxyl groups and sulfonic acid groups, it can participate in many chemical reactions, such as complexation reactions with certain metal ions, or substitution and condensation reactions with other organic compounds under specific conditions.
This substance is widely used in biochemistry and molecular biology experiments, and is often used as a buffer to create a stable acid-base environment for various biochemical reactions, ensuring the smooth progress of the reaction, and plays a crucial role in the activity and structural stability of biomolecules.

3- (N-morpholine) -2-hydroxy-1-propanesulfonic acid, often referred to as MOPSO, is synthesized as follows:
First take an appropriate amount of morpholine and slowly add it to a reaction kettle with a certain amount of epichlorohydrin in advance. The two interact, during which the reaction temperature needs to be precisely controlled, so that it is constant in a specific range, and at the same time, it is moderately stirred to ensure that the two are fully contacted and the reaction is smooth. When the reaction between the two reaches a predetermined degree, the corresponding intermediate product is formed.
Then, pour the resulting intermediate product into a solution containing sodium bisulfite. This step also requires strict control of the reaction temperature and reaction time, so that the intermediate product can fully react with sodium bisulfite. During this reaction, the structure of the intermediate product will undergo specific changes and gradually transform into the target product 3- (N-morpholino) -2-hydroxy-1-propanesulfonic acid.
When the reaction is completely over, the reaction mixture is separated and purified. Usually, the insoluble impurities are removed by filtration. Then the method of distillation is used to separate the excess reaction reagents and by-products according to the difference in boiling points of different substances. Afterwards, the method of recrystallization is used to further purify the target product to make its purity meet the corresponding standards.
In this way, high purity 3- (N-morpholino) -2-hydroxy-1-propanesulfonic acid can be obtained through the above series of steps. The whole synthesis process requires strict control of each reaction condition and careful operation of separation and purification steps to obtain the ideal product.

3- (N-morpholino) -2-hydroxy-1-propanesulfonic acid, or MOPS, is a commonly used buffer in biochemical experiments. When using, many aspects need to be paid attention to.
The first weight is its pH range. The buffer range of MOPS is about 6.5-7.9. When applying, the pH should be precisely adjusted according to the experimental requirements. To obtain a stable pH environment, it is necessary to accurately measure and adjust it with a pH meter.
The second is related to the concentration. The concentration selection varies according to the experimental system. If the concentration is too low or difficult to maintain a stable pH in protein research, and the concentration is too high or affects the activity of the protein, the appropriate concentration should be determined by pre-experiment before the experiment.
Third, pay attention to purity and impurities. High-purity MOPS can reduce the interference of impurities. Impurities or reactions with the components of the reaction system cause deviations in experimental results. Choose a reliable brand and high-purity product when purchasing, and purify it yourself if necessary.
Fourth, pay attention to solubility. MOPS is easily soluble in water. When dissolved, it can be stirred or heated to promote solubility. However, the heating temperature should not be too high to avoid decomposition or change its properties.
Fifth, pay attention to storage conditions. MOPS should be stored in a dry, cool place to avoid moisture and high temperature. If it is not stored properly, it will absorb moisture or deteriorate, which will affect the experimental effect.
Finally, the use process should follow the laboratory safety regulations and take protective measures, such as wearing gloves, goggles, etc., to prevent it from contacting the skin and eyes. If it comes into contact accidentally, it should be handled in a timely manner according to safety guidelines.