What is the main use of 3- (N-Morpholino) -2-Hydroxypropanesulfonic Acid, Sodium Salt?
Sodium 3- (N-morpholino) -2-hydroxypropane sulfonate, often referred to as "MOPSO-Na". It has a wide range of uses and is important for biochemical and molecular biology experiments.
The first to bear the brunt, the preparation of buffer is its important function. In living organisms, many biochemical reactions depend on a suitable pH environment. MOPSO-Na has good buffering properties, which can maintain the pH stability of the reaction system, making experiments such as enzymatic reactions, protein and nucleic acid studies smooth. For protein crystallization experiments, precise pH maintenance is essential for correct protein folding and crystallization, and MOPSO-Na buffer can play this role.
Furthermore, its shadow is also common in the field of cell culture. Cell growth is sensitive to environmental pH. MOPSO-Na is used in cell culture medium to create a stable pH environment and help cells grow and proliferate healthily. It can be used to maintain an appropriate pH in animal cell culture or microbial fermentation.
In addition, MOPSO-Na is also indispensable in the preparation of electrophoresis buffers. When electrophoresis separates biological macromolecules such as proteins and nucleic acids, stabilizing pH has a great impact on the molecular migration rate and separation effect. The buffer made of this salt can ensure the constant pH during electrophoresis, and obtain clear and accurate separation results.
MOPSO-Na plays a key role in many fields such as life science research, biopharmaceuticals and clinical diagnostics due to its unique properties, providing powerful tools for researchers to explore the mysteries of life.
3- (N-Morpholino) -2-Hydroxypropanesulfonic Acid, Sodium Salt What should be paid attention to during storage
3- (N - Morpholino) -2 - Hydroxypropanesulfonic Acid, Sodium Salt (3- (N-Morpholino) -2-Hydroxypropanesulfonate sodium salt), when storing, pay attention to the following numbers.
First, temperature is very important. This compound needs to be stored in a low temperature environment. Generally speaking, it should be placed in refrigeration conditions of 2-8 ° C. If the temperature is too high, it may cause changes in its chemical structure and affect its stability and quality. For example, high temperature may prompt its hydrolysis reaction, making it lose its original efficacy.
Second, moisture resistance cannot be ignored. Because it has a certain degree of hygroscopicity, if the ambient humidity is high, it is easy to absorb moisture in the air, causing it to agglomerate or even deteriorate. Therefore, it should be stored in a dry place. It can be stored in a sealed container, or a desiccant can be placed in the storage place to maintain a dry environment.
Third, avoid light. Light may cause photochemical reactions, causing damage to its structure and properties. It should be placed in an opaque container, or stored in a dark place, such as inside a cabinet, to prevent the influence of light.
Fourth, isolated from other substances. This compound may react with certain substances. When storing, it is necessary to avoid contact with chemically active substances such as acids and bases to prevent interaction and deterioration.
Only proper attention to the above storage points can ensure that 3- (N - Morpholino) -2 - Hydroxypropanesulfonic Acid, Sodium Salt maintains good quality and performance during storage.
How is the solubility of 3- (N-Morpholino) -2-Hydroxypropanesulfonic Acid, Sodium Salt?
3- (N-morpholino) -2-hydroxypropane sulfonate sodium salt, its solubility is of great concern. This salt has good solubility in water and can dissolve quickly to form a uniform solution at room temperature. Because its molecular structure contains hydrophilic groups, such as hydroxyl groups and sulfonic acid groups, it can interact with water molecules by hydrogen bonding, thereby enhancing its dispersion ability in water.
In polar organic solvents, such as methanol and ethanol, it also has a certain solubility. However, in non-polar organic solvents, such as benzene and hexane, the solubility is very poor. Because the molecular polarity does not match the non-polar solvent, it is difficult to overcome the intermolecular force and disperse it.
In practical applications, the dissolution conditions also affect. Heating can speed up the dissolution rate, and moderate stirring can also promote its rapid dissolution. However, excessive temperature or vigorous stirring may affect its chemical stability, so it needs to be considered when using it. In general, the substance has suitable solubility in water and some polar organic solvents due to the existence of hydrophilic groups, which provides convenience for its application in biochemical, pharmaceutical and other fields.
What are the chemical properties of 3- (N-Morpholino) -2-Hydroxypropanesulfonic Acid, Sodium Salt
3- (N-morpholino) -2-hydroxypropanesulfonate sodium salt, this compound is a genus of organic compounds. Its properties are quite high hydrophilicity, because it contains sulfonic acid groups and hydroxyl groups, it can be well dissolved in water to form a uniform solution. This characteristic makes it widely used in biochemical research and medicine, because many biological reactions and drug effects are carried out in aqueous media.
The compound is weakly acidic, and its morpholine ring interacts with sulfonic acid groups and hydroxyl groups, resulting in its buffering ability. In a specific pH range, the release or combination of its own protons can be used to maintain the relative stability of the pH of the system. In biological experiments or chemical reactions that require precise control of pH, it is of great significance to ensure that the reaction can be carried out in an orderly manner under a suitable pH environment, avoiding abnormal reactions or inactivation of biologically active substances due to large fluctuations in pH.
Thermal stability is also an important property. Its structure and chemical properties remain stable within a certain temperature range, and it is not easy to decompose or undergo qualitative changes. This property makes the compound suitable for experiments and production processes that require heating or high temperature treatment. It can perform its own functions under specific temperature conditions without being overly affected by temperature.
In addition, 3- (N-morpholino) -2-hydroxypropane sulfonate sodium salt has relatively mild chemical properties and is not strongly corrosive or reactive to many biomolecules and chemicals. When compatible with other substances, it is not easy to cause adverse reactions. When building complex chemical systems or biological systems, it can be used as a reliable component to facilitate the smooth development of experiments and production.
3- (N-Morpholino) -2-Hydroxypropanesulfonic Acid, Sodium Salt are widely used in which areas
3- (N-morpholine) -2 -hydroxy propane sulfonate sodium salt is widely used in biochemical research, drug development, cell culture and many other fields.
Looking at this compound, it is often used as a buffer in the field of biochemical research. Because it can effectively maintain the stability of the pH value of the system, many biochemical reactions and analytical experiments rely on it to ensure that the reaction environment is suitable, so that biomolecules maintain their inherent structure and activity. For example, in the experiment of enzyme activity determination, a suitable pH environment is the key to the full display of enzyme activity. The stable pH conditions created by this compound can make the experimental results more accurate and credible.
It also plays an important role in drug development. In the process of pharmaceutical preparation, it is necessary to adjust and maintain the appropriate pH value to ensure the stability and effectiveness of the drug. This compound can provide a stable chemical environment for the drug, prevent drug degradation or deterioration, and improve the quality and efficacy of the drug. At the same time, because of its good biocompatibility, it has little impact on organisms, and is of great value in drug research and development experiments and production.
In the field of cell culture, cell growth requires strict environmental requirements, and pH stability is particularly critical. This compound can adjust the pH value of the cell culture medium, create a suitable microenvironment for cell growth, and help cells grow, proliferate and metabolize normally. Whether it is basic cell biology research or large-scale cell culture in the biopharmaceutical process, it is indispensable.
In summary, sodium 3- (N-morpholino) -2 -hydroxypropane sulfonate is widely used in the fields of biochemistry, medicine and cell culture, and has made outstanding contributions to the research and development of related fields.
