What are the main uses of 3- (N-Morpholino) Propanesulfonic Acid?
3- (N-morpholine) propanesulfonic acid, often referred to as "MOPS". It has a wide range of uses and is particularly critical in the field of biochemical and molecular biology experiments.
The first use of buffers. In many experiments that require precise control of pH, MOPS can create and maintain a stable pH environment. Because of its specific pKa value, within a specific pH range (about 6.5 - 7.9), it can effectively resist the influence of external acid and base on the pH of the system. For example, in RNA electrophoresis experiments, the MOPS buffer system can ensure that RNA molecules can be well separated in a suitable pH environment, without degradation or abnormal migration due to pH fluctuations.
Furthermore, it is suitable for cell culture. When cells are cultured in vitro, they are extremely sensitive to environmental pH. MOPS can help maintain the pH stability of the culture medium and provide an excellent microenvironment for cell growth and proliferation. Such as mammalian cell culture, MOPS is often added to the culture medium formula to enable normal metabolism and functional operation of cells under stable pH conditions.
In addition, enzymatic reaction research is also commonly used. The activity of many enzymes is significantly affected by pH. MOPS buffer can adjust and stabilize the pH of the reaction system to ensure that the enzyme is in the best active state, enabling researchers to accurately explore the kinetic characteristics and substrate specificity of enzymes.
MOPS plays an indispensable role in biochemistry, cell biology and molecular biology research due to its excellent buffering properties, laying a solid foundation for the successful development of various experiments.
What is the pH buffer range of 3- (N-Morpholino) Propanesulfonic Acid?
3- (N-morpholinic) propanesulfonic acid, often referred to as MOPS, is a commonly used buffer in biochemical experiments. Its pH buffer range is about 6.5 to 7.9.
MOPS has many characteristics, making it suitable for buffering in a specific pH range. In this pH range, MOPS can effectively resist large changes in the pH value of the solution when a small amount of acid or base is added, thus maintaining the relative stability of the system. This buffer range meets the needs of many biochemical reactions and experiments. For example, in some enzymatic reactions, specific enzymes need to be within this pH range to exhibit the best activity. MOPS can create a suitable acid-base environment to ensure the smooth progress of the reaction. Or in cell culture-related operations, the normal growth and metabolism of cells also require specific and stable pH conditions. The MOPS buffer system can meet this requirement and provide a stable microenvironment for cells to survive. Therefore, the pH buffer range of 6.5 to 7.9 lays the important position of MOPS in the field of biochemistry and provides indispensable support for many experiments and studies.
3- (N-Morpholino) Propanesulfonic Acid Storage
3- (N-morpholino) propanesulfonic acid, or MOPS, is a commonly used biological buffer. Many matters need to be paid attention to when storing it to ensure its quality and performance.
Temperature and humidity of the first storage environment. It should be placed in a cool, dry place away from direct sunlight and heat sources. Due to high temperature, or its chemical properties become active, it will cause decomposition or deterioration; if the humidity is too high, it is easy to absorb moisture and agglomerate, which will affect the use. Usually, the storage temperature is preferably 2-8 ° C, and the humidity should be maintained at 40% -60%.
times and packaging sealing. After use, be sure to ensure that the package is tightly sealed to prevent air, moisture and other impurities from invading. If the package is damaged, it should be transferred to a sealed container in time. Because of its certain hygroscopicity, exposure to air for too long, it is easy to absorb water vapor and change the concentration and buffering performance.
Again, storage period. Even if the storage conditions are suitable, there is a shelf life. It should be checked regularly to pay attention to whether the appearance and odor are abnormal. Near the shelf life, it needs to be tested and verified before it can be used if it is qualified. Expired MOPS, or due to changes in ingredients, the buffering effect is not good, which affects the experimental or production results.
In addition, when storing, it should be separated from oxidants, acids, bases, etc. Because of its active chemical properties, contact with these substances, or chemical reaction, causing deterioration and failure. And keep away from ignition sources and flammable materials, because although they are not flammable, they are flammable in the environment or endanger safety.
Store 3 - (N - morpholino) propanesulfonic acid, and start from the aspects of temperature and humidity, packaging, duration, and item isolation to ensure its quality and use effect.
3- (N-Morpholino) Propanesulfonic Acid is harmful to human body
3 - (N -morpholinic) propanesulfonic acid, often referred to as MOPS, is harmful to the human body and must be reviewed in detail.
This substance is often used in biochemical and molecular biology experiments as a buffer, which can stabilize the pH value of the solution. Under laboratory conditions, its properties are relatively stable and usually do not cause immediate harm.
However, if the human body is exposed, the route is different and the impact is different. If it is exposed to the skin, or causes slight irritation, it will cause redness and itching of the skin. If it accidentally enters the eyes, it will cause pain, redness and swelling due to its chemical characteristics, or irritate the eye tissue, causing pain, redness and swelling, improper handling, or damage to vision.
If inhaled inadvertently, MOPS particles may irritate the respiratory tract, causing cough and asthma. For those with sensitive respiratory tract, it will have a greater impact, or induce asthma and other diseases. If eaten by mistake, or irritate the digestive system, it will cause nausea, vomiting, abdominal pain and other diseases.
Although there is no conclusive evidence that it has serious long-term carcinogenic and teratogenic hazards, it should be used with caution. Experimenters need protective equipment, such as gloves, goggles, and masks, to avoid direct contact. Operate in a well-ventilated place to prevent inhalation. If exposed, rinse with plenty of water quickly and seek medical attention if necessary.
In summary, 3- (N-morpholino) propanesulfonic acid is a non-toxic substance, but it is not used correctly and in contact with it, or it may pose a certain hazard to the human body. It must be used with caution.
What are the advantages of 3- (N-Morpholino) Propanesulfonic Acid over other buffers?
3- (N-morpholine) propanesulfonic acid, often referred to as "MOPS". Compared with other buffers, it has many advantages.
First of all, its pH buffer range, the buffer range of MOPS is about pH 6.5-7.9. This range is suitable for many biochemical reactions and biological systems. It is like a good bow of ancient times, and the range is just right in the bullseye. The enzymatic reactions and stable environments of proteins in many organisms need to be stable in this pH range. MOPS can be called a right-hand man here.
Second, in terms of its chemical stability, MOPS is not easy to react with common metal ions and oxidative reducing agents under conventional conditions. It is like a rock and is not easily shaken by foreign objects. This property allows it to stably maintain the buffer function in complex biochemical systems without interference from other substances.
Furthermore, MOPS has little interference with biochemical reactions. Many buffers may participate in or affect the biochemical reaction process due to their special structures, while MOPS is not. It is like a sage hidden behind the scenes, silently maintaining the pH stability of the system and not disturbing the normal track of the reaction. This advantage is particularly critical in experiments such as enzyme activity detection and protein crystallization.
And MOPS has good solubility, which can be well dissolved in water and common organic solvents, just like fish get water, which brings many conveniences to the experimental operation and allows researchers to more conveniently prepare the required concentration of buffer.
Therefore, 3- (N-morpholino) propanesulfonic acid is favored by researchers in the field of biochemical research due to its unique advantages, which is of great help to explore the mysteries of life.