3- (N-Morpholino) Propanesulfonic Acid Hemisodium Salt
3- (N-morpholino) propanesulfonate sodium semi-salt is the mainstay of biochemical research and biological buffer system. It has a wide range of uses and plays a crucial role in the field of life science experiments.
First, in enzymatic reaction systems, this substance is very useful. Enzymes are also catalysts for biochemical reactions, and their activity is highly susceptible to environmental pH. 3- (N-morpholino) propanesulfonate sodium semi-salt can create and maintain a stable pH environment, allowing enzymes to function at the optimum pH, thereby ensuring the smooth progress of enzymatic reactions. For example, many studies of hydrolases and oxidoreductases rely on this substance to stabilize the pH of the reaction system in order to obtain accurate experimental results.
Second, it is also indispensable in protein-related research. The structure and function of proteins are closely related to environmental pH. This salt can effectively avoid protein denaturation due to pH fluctuations, and protect the natural conformation and biological activity of proteins. Whether it is the separation and purification process of proteins, or the in-depth study of their structure and function, it is necessary to maintain a stable pH condition so that the characteristics of proteins can be accurately studied.
Third, in the field of cell culture, 3- (N-morpholino) sodium propane sulfonate semi-salt also plays a key role. Cell growth has strict environmental requirements, and a suitable pH environment is the basis for normal cell growth and metabolism. The preparation of cell culture medium with this salt can create a stable micro-environment, meet the needs of cell growth, and help the successful development of cell culture experiments.
In summary, 3- (N-morpholino) propanesulfonate sodium semi-salt is an essential substance for ensuring the smooth progress of experiments and obtaining reliable results in many fields such as biochemical research, protein research, and cell culture. Its importance is self-evident.
3- (N-Morpholino) Propanesulfonic Acid Hemisodium Salt
3- (N-morpholino) propanesulfonate sodium salt, which is a very important chemical substance. This substance has many unique chemical properties.
It is a white crystalline powder with stable properties and does not change significantly under common conditions. In terms of solubility, it can be dissolved in water to form a uniform and stable solution, which makes it widely used in reactions or experiments of many aqueous solution systems.
In terms of pH, its aqueous solution has a certain buffering ability, which can maintain the relative stability of the pH value of the solution within a specific range. This buffering performance is crucial in biochemistry and molecular biology experiments. Because many biochemical reactions need to be carried out under a specific and stable pH environment, 3- (N-morpholino) propane sulfonate sodium salt can provide such a suitable environment.
From the perspective of chemical activity, the sulfonic acid group and morpholino group in the molecular structure of this substance make it have certain reactivity, and can participate in a variety of organic synthesis reactions, such as substitution reactions with some nucleophiles, etc., which have certain application value in the field of organic synthesis. At the same time, because of its sodium content, it may also play a specific role in some reactions involving ion exchange or ion participation. In short, sodium 3- (N-morpholino) propane sulfonate has an indispensable position in many chemical and related fields due to its diverse chemical properties.
What are the storage conditions for 3- (N-Morpholino) Propanesulfonic Acid Hemisodium Salt?
Sodium 3- (N-morpholino) propanesulfonate should be stored in a cool, dry and well-ventilated place. This substance is resistant to moisture, and if it is damp, it will easily affect its chemical properties and purity. Therefore, it should be stored in a closed container to prevent moisture from invading.
Temperature is also critical and should be controlled between 2-8 ° C. Excessive temperature may cause decomposition to accelerate, and too low temperature may change some physical properties.
At the same time, keep away from fire and heat sources, because its chemical structure may be flammable and thermally sensitive under specific conditions. The storage environment should also avoid contact with strong oxidants, strong acids, strong bases and other substances to prevent chemical reactions from occurring and causing deterioration.
When using this substance, be sure to ensure that the operating environment is dry, the utensils used are clean and dry, and seal the container in time after use to maintain its stable storage environment, so as to ensure the quality and performance of 3 - (N - morpholino) propionate sodium salt are stable for a long time.
What are the precautions for the use of 3- (N-Morpholino) Propanesulfonic Acid Hemisodium Salt in the experiment
3- (N-morpholino) propanesulfonate sodium salt in the experiment, use it with caution, there are all kinds of precautions.
First, the solubility of this substance should be paid attention to. Although it has a certain solubility in water, when it dissolves, the water temperature and stirring rate are affected. If the water temperature is too low, the dissolution is slow; stirring too much, or causing the solution to splash out. It is advisable to use moderately warm water and stir it slowly to help it dissolve into a uniform solution.
Second, the regulation of pH value is important. 3- (N-morpholino) propanesulfonate sodium salt is often used as a buffer, and its buffer range is specific. In the experiment, it is necessary to precisely prepare according to the required pH value, otherwise it may affect the reaction process. And after the preparation, when the pH meter is re-tested to ensure that it is correct.
Third, the stability should not be ignored. This salt is left in the air for a long time, or deteriorated due to humidity, temperature, oxidation, etc. Therefore, it should be stored in a dry and cool place, and sealed immediately after use to prevent its effectiveness.
Fourth, compatibility with other reagents. It is often mixed with other substances in the experiment, and it is necessary to clarify whether it is compatible with the reagents used before use. If not, it may cause abnormalities such as precipitation and discoloration, which will damage the experimental results.
In conclusion, the use of 3- (N-morpholino) propane sulfonate sodium salt requires caution in dissolution, pH regulation, storage, and reagent compatibility to ensure smooth experimentation and certainty of results.
3- (N-Morpholino) Propanesulfonic Acid Hemisodium Salt Reacts with Other Chemicals
3- (N - Morpholino) Propanesulfonic Acid Hemisodium Salt (3- (N - Morpholino) Propanesulfonic Acid Hemisodium Salt) is often used in biological buffer systems. This substance can react with many chemical substances, as follows:
** Reaction with acids **: As a weak acid salt, it will react with strong acids. For example, when it encounters hydrochloric acid, it will generate 3- (N - Morpholino) propanesulfonic acid (MOPS) and corresponding sodium salts according to the principle of strong acid to weak acid. This reaction can be used to prepare MOPS, and the reaction formula that occurs is roughly: $C_7H_ {14} NO_4SNa + HCl\ longrightarrow C_7H_ {15} NO_4S + NaCl $.
** Reaction with bases **: If a strong base is encountered, there are groups in the molecule that can react with bases. For example, the nitrogen atom on the morpholine ring can undergo proton transfer with the base, resulting in changes in molecular structure and properties. However, the specific reaction will vary depending on the type of base and the reaction conditions.
** Reaction with metal ions **: It can complex with some metal ions by means of groups such as sulfonate in the molecule. For example, with metal ions such as copper ions and zinc ions, complexes will be formed. This reaction may be used in the detection or separation of some metal ions, and the generated complexes will show unique characteristics in terms of structure and stability.
** Reaction with active halogenated hydrocarbons **: Under appropriate conditions, the nitrogen atom on the morpholine ring has nucleophilicity, which can undergo nucleophilic substitution reactions with active halogenated hydrocarbons, allowing molecular structures to be expanded and derived, and then compounds with different functions can be obtained, which has certain application value in the field of organic synthesis.