3- (N-Morpholino) Propanesulfonic Acid, What is the use of Na
3- (N-morpholino) propanesulfonic acid (MOPS) sodium salt is widely used in the field of biochemistry and molecular biology.
In the buffer system, its function is significant. Because MOPS-Na has a suitable pKa value, about 7.2-7.4, it can show good buffering ability in the pH range near neutral. Many biochemical reactions, such as enzymatic reactions, are extremely sensitive to the pH of the reaction environment, and small changes may affect the activity and reaction process of enzymes. MOPS-Na can maintain the pH of the system and ensure the smooth progress of enzymatic reactions. For example, in the DNA restriction endonuclease reaction, the pH is precisely regulated to create conditions for the best activity of the endonuclease and facilitate the accurate cutting of DNA fragments.
In the field of cell culture, it is also indispensable. Cell growth requires harsh environmental requirements, and pH stability is essential. Adding MOPS-Na to the cell culture medium can prevent large fluctuations in pH due to factors such as the accumulation of cell metabolites, create a suitable growth microenvironment for cells, maintain normal physiological functions and metabolic activities of cells, and ensure the smooth development of cell culture.
In electrophoresis buffer, MOPS-Na is also useful. For example, during RNA electrophoresis, MOPS-Na participates in the construction of a buffer system, stabilizes the pH of the electrophoresis process, and ensures the stable migration rate of RNA molecules in the electric field, so that RNA fragments of different sizes can be effectively separated, which is conducive to subsequent detection and analysis.
In addition, during the protein purification process, MOPS-Na buffers can avoid protein denaturation due to pH changes, maintain protein structure and function integrity, and help obtain high-purity, high-activity proteins.
What are the physicochemical properties of 3- (N-Morpholino) Propanesulfonic Acid, Na
3- (N-morpholinyl) sodium propanesulfonate, the physical and chemical properties of this substance are as follows:
Its appearance is usually white crystalline powder, which is relatively stable at room temperature and pressure. From the perspective of solubility, it is easily soluble in water, which makes it well dispersed and functional in experimental or industrial applications of aqueous solution systems.
As far as pH is concerned, it is a good biological buffer, which can effectively maintain the pH stability of the solution within a specific pH range. Generally, its effective buffer range is between pH 6.5 and 7.9, which can resist the influence of a small amount of external acid-base substances on the pH of the system, just like a dam to resist floods, to ensure the smooth progress of biochemical reactions or other pH-sensitive processes.
In terms of melting point, it has a relatively clear melting point, which helps to provide a basis for judging its quality in analytical work such as identifying its purity through melting point determination.
In terms of chemical stability, under conventional chemical environment and operating conditions, it is not easy to decompose, oxidize and other chemical reactions, and can maintain its own chemical structure and properties relatively stable, so that it can maintain its efficacy and characteristics for a long time during storage and use, providing a reliable guarantee for the smooth development of related work.
3- (N-Morpholino) Propanesulfonic Acid, Na What are the precautions when storing
3 - (N -morpholine) sodium propanesulfonate, when hiding, pay attention to a few things.
This agent should be stable in nature, and it is afraid of heat and moisture. Therefore, store in a cool and dry place, the temperature should be controlled between 15 and 30 degrees Celsius, and the humidity should not exceed 60%. High temperature will easily promote its change, and the tide will rise or cause poor quality, which will damage its effectiveness.
It is also necessary to avoid co-storage with strong oxidants and strong alkalis. These two are strong in nature, and it is dangerous to encounter them or to react to them. Store in a place that is open and protected from direct sunlight, and light and heat can also cause them to deteriorate.
The packaging must be tight to prevent its leakage. If the packaging is broken, the agent is exposed to the outside, easy to be contaminated, and scattered in the environment, or harmful to the ecology.
Furthermore, the place of placement should be clearly marked, so that people can easily identify it, prevent misuse and misuse, and avoid accidents. When handling, you should also be careful and move it lightly to avoid shock damage. In this way, the quality of sodium 3 - (N-morpholino) propanesulfonate is guaranteed when stored, and it is effective and safe to use.
How to prepare 3- (N-Morpholino) Propanesulfonic Acid, Na
To prepare 3- (N - Morpholino) Propanesulfonic Acid (Na), you can follow the following method.
Prepare an appropriate amount of 3- (N - Morpholino) Propanesulfonic Acid (MOPS) and place it in a clean container. Add an appropriate amount of deionized water slowly and stir gently to fully dissolve the MOPS to obtain a uniform solution.
Take an appropriate amount of sodium hydroxide (NaOH) and deionized water to prepare a certain concentration of sodium hydroxide solution. In this process, caution is required because sodium hydroxide is corrosive.
Slowly add the prepared sodium hydroxide solution dropwise while stirring the MOPS solution continuously. The rate of dropwise addition should be slow to prevent the reaction from being too violent. During this process, the pH value of the solution is monitored in real time. When the pH value reaches the desired range, a solution of sodium 3- (N-morpholino) propionate is obtained.
If you want to obtain a solid of sodium 3- (N-morpholino) propionate, the resulting solution can be removed by vacuum distillation or low temperature evaporation. When the crystals are precipitated, the crystals can be separated from the mother liquor by means of filtration. The resulting crystals are washed with an appropriate amount of cold deionized water or organic solvent to remove impurities. After that, the crystals are placed in an oven and dried at a suitable temperature until constant weight to obtain a pure 3- (N-morpholino) sodium propane sulfonate solid.
During the whole operation, pay attention to safety, wear protective equipment, and strictly follow the experimental procedures to ensure that the product is pure and the operation process is smooth.
3- (N-Morpholino) Propanesulfonic Acid, Na is widely used in which fields
3- (N-morpholine) propanesulfonic acid (MOPS) sodium salt is widely used in many fields such as biochemistry and molecular biology.
In biochemical experiments, it is often used as a buffer. Because it can effectively maintain the stability of the pH of the solution within a specific pH range (about 6.5-7.9), it is like the ancient "fixed sea needle" to ensure the stability of many biochemical reactions. For example, enzymatic reactions, enzyme activities are extremely sensitive to pH. The stable acid-base environment created by MOPS sodium salt can enable enzymes to exert the best activity and ensure the smooth reaction.
In the field of molecular biology, nucleic acid and protein research are also indispensable. Nucleic acid extraction, purification and PCR amplification all require a suitable pH environment. MOPS sodium salts can provide stable conditions for these operations, ensuring that the structure and function of nucleic acids are not affected by pH. In protein electrophoresis experiments, it can maintain the pH stability of the buffer system, and help proteins to effectively separate according to different charges and molecular weights, just like guiding the direction of protein separation.
In the field of cell culture, MOPS sodium salts escort cell growth. Cell growth requires strict environmental pH, and it can adjust the pH of the medium to create an environment suitable for cell survival and proliferation. For example, it can build a comfortable "home" for cells, so that cells can grow healthily.
In microbiology research, MOPS sodium salts are also used to control the pH of the medium when cultivating microorganisms. Different microorganisms have different preferences for pH, which can meet the growth needs of specific microorganisms and promote microbial culture and research.
In addition, in the fields of drug research and development, food testing, etc., due to the need to precisely control the pH of the reaction system, MOPS sodium salts are also widely used, contributing to the development of various fields.