3- (N-Morpholino) Propanesulphonic Acid Monosodium Salt
3- (N-morpholino) propanesulfonate sodium salt, this is a very important chemical substance, which is widely used in many fields.
In biochemistry and molecular biology experiments, it often acts as a buffer. Because many biochemical reactions in organisms are extremely sensitive to pH, they need to be in a specific pH range to proceed smoothly. This compound can effectively maintain the pH stability of the reaction system, ensuring that experiments such as enzymatic reactions, protein and nucleic acid research can be carried out accurately. For example, when studying the activity of a specific enzyme, by adjusting the concentration of 3- (N-morpholino) propane sulfonate sodium salt in the buffer, a pH environment suitable for the enzyme to exert the best activity can be created, and then the catalytic mechanism and kinetic properties of the enzyme can be accurately explored.
In the field of pharmaceutical research and development, it also has important applications. In the process of pharmaceutical preparation, its buffer performance can be used to adjust the pH value of the drug solution and improve the stability and solubility of the drug. Some drugs are easier to dissolve and absorb under specific pH conditions. With the help of this compound, the pH environment of the pharmaceutical preparation can be optimized, which helps to enhance the efficacy of the drug and reduce adverse reactions.
In addition, in the field of cell culture, the growth and metabolism of cells have strict requirements on the pH of the environment in which they are located. As a key component of the buffer system, 3- (N-morpholino) propane sulfonate can create a stable acid-base environment for cells, ensure normal cell growth, proliferation and differentiation, and assist in the research of cell biology and the production of biological products.
3- (N-Morpholino) Propanesulphonic Acid Monosodium Salt
3- (N-morpholino) propanesulfonate sodium salt is a commonly used reagent for biochemical research. Its physical and chemical properties are as follows:
Appearance properties, usually white crystalline powder, fine and uniform texture, this form is easy to accurately weigh and experimental operation.
Good solubility, easy to dissolve in water, can quickly disperse in water and form a uniform solution, which is conducive to play a role in various aqueous experimental systems. Its aqueous solution is nearly neutral. Due to the existence of sulfonic acid groups and morpholine rings in the molecular structure, the substance has a certain degree of ionization in water, which in turn affects the pH of the solution.
In terms of melting point, it has a relatively clear melting point range. This characteristic can be used to identify the purity of the substance. The melting point of high-purity samples is more acute and close to the theoretical value.
has good stability. Under conventional storage conditions, in a dry and cool place, it can maintain chemical stability for a long time and is not easy to decompose or deteriorate. However, it is necessary to avoid contact with strong oxidants, strong acids, strong bases and other substances to prevent chemical reactions from causing changes in its structure and properties.
In addition, its molecular structure gives it unique buffering ability, which can effectively maintain the stability of solution pH within a specific pH range. It is of great significance in many biochemical reactions and experimental systems. It can provide a stable chemical environment for biomolecules and ensure the smooth progress of experiments.
3- (N-Morpholino) Propanesulphonic Acid Monosodium Salt
3- (N-morpholino) propanesulfonate sodium salt, this is a commonly used reagent for biochemical experiments. To properly store, you need to pay attention to the following things:
First, store it in a dry place. This reagent is prone to moisture absorption, and moisture may change its properties and affect the experimental results. Therefore, it should be placed in a dryer or a place with controlled humidity to keep it dry.
Second, store it at controlled temperature. Generally speaking, it should be stored in a low temperature environment, generally 2-8 ° C. If the temperature is too high, it may decompose and deteriorate the reagent, reducing its stability. Place it in the corresponding low temperature refrigerator to protect its quality.
Third, store it in the dark. Light or initiate a chemical reaction of the reagent to make it ineffective. Therefore, it should be placed in a brown bottle or opaque container and stored in a dark place to prevent light from damaging it.
Fourth, sealed storage. In order to avoid oxidation caused by contact with the air or absorption of components in the air, it must be well sealed. After taking it, seal it immediately, and do not expose the reagent to the air for a long time.
Fifth, arrange it in categories. Place it separately from other reagents, especially avoid coexisting with reactive reagents to prevent dangerous interaction or deterioration of reagents. According to the nature and type of reagents, store it in an orderly manner, and make use of and manage it.
In this way, careful and thorough storage can ensure the quality and performance of 3 - (N - morpholino) propane sulfonate sodium salt, and play its due role in the experiment.
3- (N-Morpholino) Propanesulphonic Acid Monosodium Salt
The price range of "3 - (N - morpholine) propanesulfonate sodium salt" is difficult to determine. There are three reasons: First, the purity of this compound has a great impact on the price. If the purity is extremely high, it is almost medicinal or scientific research grade, and can be accurately used for high-end experiments and pharmaceutical research and development, the price will be high; conversely, if the purity is slightly lower, it is only for general industrial use, and the price will be low. Second, the market supply and demand relationship also determines its price. When the market demand for this product is strong and the supply is limited, if it encounters a large number of purchases in a specific industry, the price will rise; if the supply exceeds the demand, the merchant may sell it for promotion, or the price will be reduced. Third, the production cost of different manufacturers is different. Those with advanced technology and large scale may be able to reduce costs, and product pricing also has advantages; small factories are limited by technology and scale, and the cost is higher, and the price may be relatively high.
The concept of "Tiangong Kaiwu", although it does not include the price of this product, it talks about all products, and it all talks about the relationship between process, quality and value. By analogy, "3 - (N - morpholino) propionate sodium salt" If the process is complicated and the quality is excellent, the price will be high; otherwise, it will be low. Generally speaking, in the specifications commonly used in laboratories, those with high purity range from tens of yuan to hundreds of yuan per gram; those with industrial grade and slightly lower purity range from hundreds of yuan per kilogram or kilogram. However, this is only an approximate figure. The actual price needs to be consulted with each supplier and determined according to the market conditions at the time of the specific transaction.
3- (N-Morpholino) Propanesulphonic Acid Monosodium Salt is widely used in which fields
3- (N-morpholino) propanesulfonate sodium salt, this substance is widely used in many fields such as biochemical research, molecular biology, pharmaceutical research and development, biopharmaceutical and cell culture.
In biochemical research, because it can effectively maintain the pH stability of the system and can simulate the acid-base environment in organisms, it is often used in enzyme activity research. Many enzymes can only show the best activity in a specific pH range, and 3- (N-morpholino) propanesulfonate sodium salt can create a suitable environment for it, helping researchers to accurately explore the mechanism and kinetic properties of enzymatic reactions.
In molecular biology experiments, such as DNA, RNA extraction and purification, as well as PCR, electrophoresis and other experimental links, pH environment needs to be stabilized. This substance can ensure the integrity of the structure and stability of the nucleic acid during extraction and operation, prevent the degradation or denaturation of the nucleic acid due to pH fluctuations, and ensure the reliability and repeatability of the experimental results.
In the field of pharmaceutical research and development, the drug development process involves a large number of in vitro experiments and cell experiments. Sodium 3 - (N - morpholino) propane sulfonate can simulate the pH of the human physiological environment, providing stable conditions for drug activity research, drug screening and drug action mechanism exploration, and helping to more accurately evaluate the effect and safety of drugs.
In the field of biopharmaceuticals, whether it is the production of recombinant proteins, antibodies and other biological drugs, or vaccine preparation, the pH requirements of the production environment are strict. The substance can maintain a stable pH during fermentation, purification and preparation, ensure the quality and activity of biological drugs, and improve the purity and stability of products.
Cell culture is an important means of biological research, and cell growth is extremely sensitive to environmental pH. 3- (N-morpholino) propionate sodium salt can adjust the pH of cell culture medium, create a suitable microenvironment for cell growth, promote cell adhesion and proliferation, and prevent cells from growth inhibition and apoptosis due to pH discomfort. It is widely used in various cell line cultures.