3-N-Morpholinopropanesulfonic Acid,Sodium Salt

3-N-morpholine propane sulfonate sodium salt, its main uses are as follows:
This is a commonly used reagent for biochemical research. In biochemical and molecular biology experiments, it is often used as a buffer. Many chemical reactions in organisms are extremely sensitive to pH, and this substance can maintain the stability of the pH value of the reaction system, just like the cornerstone of stability, so that the reaction can proceed smoothly and orderly.
In the study of proteins and enzymes, its utility is very critical. The activity and structure of proteins and enzymes are very sensitive to changes in environmental pH, and they will be inactivated or structural changes if they are not careful. With its good buffering properties, sodium 3-N-morpholine propane sulfonate creates a suitable pH environment for proteins and enzymes to ensure their normal function, which is convenient for researchers to further explore their characteristics and mechanisms.
In the field of cell culture, cell growth also has strict requirements on the pH value of the environment. It can effectively regulate the pH value of cell culture medium, create a stable growth environment for cells, and help cells grow and proliferate healthily. It is an important guarantee for the smooth development of cell culture experiments.
In addition, in the process of drug development, when simulating the human physiological environment, it can precisely regulate the pH value of the reaction system as a buffer, which is in line with human physiological conditions, and helps to study the way and effect of drugs in the body, providing strong support for the development of new drugs.

3-N-morpholine propane sulfonate sodium salt, its physical and chemical properties are as follows:
This salt is mostly white crystalline powder with pure appearance and less variegation and foreign matter. In terms of solubility, it is easily soluble in water, and the aqueous solution is clear and transparent. This is due to the strong interaction between the polar groups contained in its molecular structure and the water molecules, which can make the molecules uniformly dispersed in water.
Its stability is good. Under conventional storage conditions, it can maintain its chemical properties for a long time in a dry and cool environment. However, it is necessary to avoid contact with strong oxidizing agents, strong acids and bases, etc., to prevent chemical reactions and destroy its structure.
From the pH point of view, it is often used as a biological buffer, which can effectively maintain the pH value of the solution within a certain range. Its buffering ability is derived from a specific acid-base balance system in the molecular structure, which can neutralize a small amount of acid or base added, so that the pH of the solution does not fluctuate greatly. Generally, its effective buffering range is between about 6.5 and 7.9 pH value. In this range, it can provide a suitable and stable acid-base environment for biochemical reactions, cell culture, etc., to ensure the activity of biomolecules and the normal progress of the reaction.
In addition, the salt has a high melting point and requires a phase transition under specific high temperature conditions. This characteristic makes it stable in the solid state at room temperature and most conventional experimental and production environment temperatures, which is convenient for storage and access.

When storing and transporting 3 - N - Morpholinopropanesulfonic Acid sodium salt, there are many key things to pay attention to.
First, temperature control is essential. This substance should be stored in a cool and dry place. Generally speaking, the temperature should be maintained at 2 - 8 ° C. If the temperature is too high, or its chemical properties change, such as decomposition, which will affect its quality and use effect. If the temperature is too low, although it will not freeze, it may affect its physical state and make it inconvenient to use.
Secondly, the humidity factor cannot be ignored. Be sure to ensure that the storage environment is dry, because it is hygroscopic. If the ambient humidity is high, it is easy to absorb moisture, resulting in agglomeration, which not only destroys its original form, but also may affect its purity and stability. When transporting, it should also be prevented from moisture, and measures such as sealed packaging can be taken.
Furthermore, the influence of light should not be underestimated. This sodium salt should be avoided from being directly exposed to strong light, and its structure and properties should be changed due to light or luminescent chemical reactions. Therefore, it should be stored in a light-shielding container or in a dark place.
In addition, the integrity of the packaging is of great importance. During storage and transportation, the packaging must be intact to prevent leakage. Once leaked, it may not only pollute the environment, but also cause material loss, and the substance may cause certain harm to the human body and the environment.
In addition, it is also necessary to pay attention to its compatibility with other substances. Do not store or mix with strong oxidants, strong acids, strong bases, etc., because of their chemical properties or violent reactions with these substances, resulting in safety accidents. When transporting, also follow relevant regulations and place them properly to avoid collisions and vibrations to ensure transportation safety. In this way, 3-N-morpholine propionate sodium salt can maintain good quality and performance during storage and transportation.

3-N-Morpholinopropanesulfonic Acid, Sodium Salt, often abbreviated as MOPS sodium salt, is a commonly used buffer in biochemical research. The preparation method is as follows:
First, 3-morpholine propanesulfonic acid (MOPS acid) is used as the starting material. An appropriate amount of 3-morpholine propanesulfonic acid is placed in the reaction vessel. The appearance of this substance is a white crystalline powder with a certain water solubility. Slowly add sodium hydroxide (NaOH) solution to it. This step requires strict control of the amount and addition speed of sodium hydroxide. Because the reaction is an acid-base neutralization reaction, if the amount of alkali is too much or too fast, it is easy to cause the system to be too alkaline and affect the purity of the product. The reaction equation is roughly: MOPS - H + NaOH → MOPS - Na + H 2O O.
During the reaction process, the pH change of the reaction solution can be monitored in real time with the help of a pH meter. When the pH reaches a certain range (usually close to the pH value required for the product), the reaction can be considered basically complete. In order to obtain a pure sodium salt of 3 - N - morpholinpropane sulfonate, the reaction mixture needs to be treated. Filtration is first used to remove insoluble impurities that may exist. Then, evaporation and concentration are used to gradually evaporate the water in the solution to increase the concentration of the product. When the solution reaches a certain supersaturation state, crystals will precipitate.
Then by cooling the crystallization operation, reduce the temperature of the solution and promote more crystals to precipitate. This process can be carried out in a low temperature environment (such as a refrigerator freezer). After a large number of crystals have precipitated, the crystals are separated from the mother liquor by suction filtration or centrifugation. The resulting crystals still contain some impurities and water, and need to be washed with an appropriate amount of organic solvent (such as ethanol, etc.) to further remove impurities. Finally, the washed crystals were placed in an oven, dried at a suitable temperature, and the residual solvent and water evaporated to obtain a pure 3-N-morpholine propane sulfonate sodium salt product.

3-N-Morpholinopropanesulfonic Acid sodium salt is often used as a biological buffer in the field of chemistry. The compatibility of this substance with other chemicals depends on the specific substance.
If it coexists with strong acids and bases, because of its buffering characteristics, it will react with strong acids and bases to maintain pH stability of the system, so it is incompatible. For example, strong acids and bases such as sulfuric acid and sodium hydroxide will undergo acid-base neutralization with 3-N-morpholine propane sulfonate sodium salt, changing the structure and properties of the buffer.
And with some neutral salts, such as sodium chloride, potassium chloride, etc., it is generally safe. Due to the absence of precipitation, gas generation or redox reactions between ions, they can coexist in the system.
For some compounds with special active groups, the situation is different. If it contains groups that can react with sulfonic acid groups and morpholine groups in 3-N-morpholine propionate sodium salt, such as some active halogenated hydrocarbons that are prone to substitution reactions with sulfonic acid groups, it is incompatible. However, compounds such as glucose, which are relatively stable and have no active reactive groups, are generally compatible with 3-N-morpholine propionate sodium salt.
In summary, the compatibility of 3-N-morpholine propane sulfonate sodium salt with other chemicals requires comprehensive consideration of the chemical structures and properties of both parties, and cannot be generalized.